Bioanalytical method validation for the quantification of the chlorogenic acid in Capsicum baccatum through High Performance Liquid Chromatography (HPLC-DAD)

Mendes, Nathânia S.; Pereira, Silvia M.F.; Arantes, Mariana B. S.; Glória, Lorena Lima; Nunes, Clara dos Reis; Passos, Michel de Souza; Vieira, Ivo José Curcino; Moraes, Luana Pereira de; Rodrigues, Rosana; Oliveira, Daniela Barros de

doi:10.1016/j.foodchem.2020.126929

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…Table shows that the RSD values obtained with the corrected signals (S/V) are indeed low. This indicates that correcting the colorimetric signal S by the sample volume can reduce the effect of varying sample volumes in our lactate measures with an acceptable precision (RSD < 15%) . All in all, these experiments verify our initial hypothesis that the proposed analytical platform can quantify changes in the concentration of lactate even at different sample volumes thanks to combining lactate and volume measures.…”

Section: Resultsmentioning

confidence: 99%

Wearable Analytical Platform with Enzyme-Modulated Dynamic Range for the Simultaneous Colorimetric Detection of Sweat Volume and Sweat Biomarkers

2020

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In this manuscript, we introduce a wearable analytical platform that simultaneously measures the concentration of sweat lactate and sample volume. It contains two sensors entirely made of filter paper that can be easily affixed on the skin with medical-grade tape. The lactate biosensor features a unique signal modulation mechanism that enables fine-tuning the dynamic range. It consists of adding a competitive enzyme inhibitor in different reservoirs. Thanks to this, it is possible to choose between a very low limit of detection (0.06 mM) and a linear response in the physiological concentration range (10–30 mM). The sweat volume sensor was obtained by adding a reservoir containing gold nanoparticles. As the wearer sweats, the nanoparticles are carried through a paper channel. This is used to gauge the volume of sample by measuring the distance traveled by the nanoprobes. Using fine-tuned lactate biosensors and combining them with the volume sensors allowed us to quantify variations in the levels of sweat lactate independently of the wearer’s sweat rate during an exercise routine. The platform design can be customized to meet the end user’s needs, which makes it ideal for developing a wide array of disposable wearable biosensors.

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Section: Resultsmentioning

confidence: 99%

Wearable Analytical Platform with Enzyme-Modulated Dynamic Range for the Simultaneous Colorimetric Detection of Sweat Volume and Sweat Biomarkers

2020

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“…Hydroxylation of cinnamic acid, for example, supplies p ‐cumaric acid. One of the biosynthetic destinations of p ‐cumaric acid is the biosynthesis of chalcones that are the precursors of all flavonoids (Mendes et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Stability of uvaia (Eugenia pyriformis Cambess) pulp subjected to freezing by static and forced air

Macedo

Vilela

Cunha

et al. 2022

J Food Process Engineering

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Uvaia is a highly perishable exotic fruit. The use of preservation techniques is an important strategy to make uvaia pulp consumption and commercialization viable. This study aimed to evaluate the effect of freezing by static air (SA) and forced air (FA) on the physical, chemical and microbiological stability of uvaia (Eugenia pyriformis Cambess) pulp during 12 months of storage. The uvaia pulp exhibited color changes with a tendency to darkening, reduced pH, carotenoids, total phenolics, vitamin C, and antioxidant activity (AA) and increased titratable acidity throughout storage, despite the freezing method, although the soluble solids content was not influenced by any of the factors. Five phenolic and 12 volatile compounds were identified, the majority being gallic and chlorogenic acids, among the phenolics and ethyl and octanol hexanoate, among the volatiles. The method of freezing by FA was more effective than by SA in retaining phenolics, carotenoids, antioxidants and volatile activity, during storage. Good manufacturing practices guaranteed the microbiological quality of the pulp, preserved for 12 months, despite the freezing method. Frozen uvaia pulp can be considered a vehicle of bioactive compounds, with AA, microbiologically safe, even after 12 months of storage. Practical Applications The uvaia pulp has a peculiar flavor and aroma and can be used as an alternative raw material to conventional options in different products (yogurts, ice cream, fermented drinks, juices, and jellies). As it is a seasonal and extremely perishable fruit, freezing the pulp is an alternative to prolong the useful life of the uvaia. Qualitative parameters can be affected by the storage time and freezing method applied. Therefore, the evaluation of the influence of these factors on the quality of uvaia pulp is relevant and can be applied in the food industry to maintain the quality of the pulp.

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“…To date, there are few reports about the pharmacokinetics of 5-CQA. According to existing research, it is difficult to quantify 5-CQA at low concentrations due to the limitation of the instruments and equipment at the time, and the detection time is also long [ 15 , 16 ], or it may not be possible to quantify 5-CQA quickly and sensitively because of the need to detect multiple compounds at the same time [ 17 , 18 , 19 ]. Therefore, the 5-CQA quantitative method still needs to be optimized, such as by optimizing its quantitative limit and shortening its detection time.…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Sensitive UHPLC–MS/MS Method for Determination of Chlorogenic Acid and Its Application to Distribution and Neuroprotection in Rat Brain

Bai

Zhou

et al. 2023

Pharmaceuticals

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Chlorogenic acid (5-CQA) is a phenolic natural product that has been reported to improve neurobehavioral disorders and brain injury. However, its pharmacokinetics and distribution in the rat brain remain unclear. In this study, we established a rapid and sensitive UHPLC–MS/MS method for the determination of 5-CQA in rat plasma, cerebrospinal fluid (CSF), and brain tissue to investigate whether it could pass through the blood–brain barrier (BBB) and its distribution in the rat brain, and a Caenorhabditis elegans (C. elegans) strain paralysis assay was used to investigate the neuroprotective effect of 5-CQA in different brain tissues. Chromatographic separation of 5-CQA and glycyrrhetinic acid (GA, used as internal standard) was completed in 0.5 min, and the full run time was maintained at 4.0 min. Methodological validation results presented a high accuracy (95.69–106.81%) and precision (RSD ≤ 8%), with a lower limit of quantification of 1.0 ng/mL. Pharmacokinetic results revealed that 5-CQA can pass through the BBB into the CSF, but the permeability of BBB to 5-CQA (ratio of mean AUC0-∞ of CSF to plasma) was only approximately 0.29%. In addition, 5-CQA can penetrate into the rat brain extensively and is distributed with different intensities in different nuclei. A C. elegans strain paralysis assay indicated that the neuroprotective effect of 5-CQA is positively correlated with its content in different brain tissues. In conclusion, our study for the first time explored the BBB pass rate and brain tissue distribution of 5-CQA administered via the tail vein by the UHPLC–MS/MS method and investigated the potential main target area of 5-CQA for neuroprotection, which could provide a certain basis for the treatment of nervous system-related diseases of 5-CQA.

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Bioanalytical method validation for the quantification of the chlorogenic acid in Capsicum baccatum through High Performance Liquid Chromatography (HPLC-DAD)

Cited by 9 publications

References 13 publications

Wearable Analytical Platform with Enzyme-Modulated Dynamic Range for the Simultaneous Colorimetric Detection of Sweat Volume and Sweat Biomarkers

Wearable Analytical Platform with Enzyme-Modulated Dynamic Range for the Simultaneous Colorimetric Detection of Sweat Volume and Sweat Biomarkers

Stability of uvaia (Eugenia pyriformis Cambess) pulp subjected to freezing by static and forced air

A Rapid and Sensitive UHPLC–MS/MS Method for Determination of Chlorogenic Acid and Its Application to Distribution and Neuroprotection in Rat Brain

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