Bioaerosol levels and the indoor air quality of laboratories in Bangkok metropolis

Kallawicha, Kraiwuth; Chao, Hsing‐Lung; Kotchasatan, Natkrita

doi:10.1007/s10453-018-9535-5

Cited by 22 publications

(12 citation statements)

References 41 publications

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“…Similarly, Kallawicha et al (2019) observed that Aspergillus/Penicillium spores were the most abundant fungal spore taxa in the laboratories (40.6%), followed by Cladosporium (30%) and ascospores (17%).…”

Section: Predominant Genera Of Airborne Fungimentioning

confidence: 69%

Microbial diversity of bioaerosol inside sports facilities and antibiotic resistance of isolated Staphylococcus spp.

2019

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In the modern world, healthy habits and physical and mental fitness are more important than ever. A growing number of people participate in sports to improve their overall health. However, the conditions in which people exercise are seldom examined. It is obvious that the air in buildings, including sports facilities, can be contaminated with pathogenic microorganisms, causing infections and allergies. Our study was aimed at assessing microbial air quality inside several sports facilities (fitness room, martial arts room, swimming pool, sports hall, gym) and at a sports field. Another objective was to evaluate the antibiotic resistance of isolated Staphylococcus strains. Air samples were collected with MAS-100 sampler, using selective substrates. Antibiotic resistance of mannitol-positive staphylococci was assessed using a disk diffusion method in accordance with EUCAST recommendations. The results indicated large fluctuations in average concentrations of heterotrophic bacteria, ranging from 38 CFU m -3 (swimming pool) to 1036 CFU m -3 (sports hall). Generally, bacteria were more abundant inside the buildings, while fungi in the sports field (658 CFU m -3 on average). In all facilities, airborne fungal communities were dominated by the genus Cladosporium, followed by Penicillium, Fusarium and Acremonium. Alternaria and Aureobasidium constituted only a small percentage of isolated molds. We recorded only low concentrations of mannitol-positive staphylococci (on average ranging from 1 CFU m -3 at the swimming pool and sports field to 9 CFU m -3 in the martial arts room). Of all isolated Staphylococcus strains, 73% were resistant to benzopenicillin, while more than 90% were sensitive to gentamycin, levofloxacin and rifampicin.

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Section: Predominant Genera Of Airborne Fungimentioning

confidence: 69%

Microbial diversity of bioaerosol inside sports facilities and antibiotic resistance of isolated Staphylococcus spp.

2019

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“…Air quality research conducted by Sheik et al [33] in Saudi Arabia showed a similar average concentration of bacterial bioaerosol in classrooms-i.e., 290 CFU m −3 . Much lower average concentrations of heterotrophic bacteria-i.e., 87 CFU m −3 -were recorded by Kallawicha et al [34] in laboratories in Bangkok, Thailand, which can be associated with different climate, type of ventilation, number of users and their activities. Brągoszewska and Biedroń [11] emphasize the importance of adequate ventilation for microbiological air quality.…”

Section: Bacterial Bioaresol Concentrationmentioning

confidence: 95%

Diversity of Bioaerosols in Selected Rooms of Two Schools and Antibiotic Resistance of Isolated Staphylococcal Strains (Bydgoszcz, Poland): A Case Study

2020

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The present study is aimed at evaluating microbiological air pollution in libraries, cafeterias and selected classrooms of two schools in Bydgoszcz city, northern Poland and determining the antibiotic resistance of Staphylococcal strains isolated from the indoor air. One of the investigated schools (School A) is located in the very center of the city, in the vicinity of a park, among old houses and stone-lined streets, while the other (School B), among modern residential buildings, close to a street with heavy traffic. In each school, air samples were collected in the morning, always from all three sampling sites, using the MAS-100 sampler. Selective growth media were used for bacteria and mold isolation and quantifying analysis. The antibiotic resistance of the isolated mannitol-positive staphylococci was assessed using the disc diffusion method in accordance with EUCAST recommendations. The highest mean concentration of heterotrophic bacteria was recorded in the cafeterias: 884 CFU m−3 in School A and 1906 CFU m−3 in School B. Molds were the most abundant in the library and cafeteria in School B, where their average concentration exceeded 300 CFU m−3. Cladosporium and Penicillium species prevailed, while Fusarium, Acremonium and Aspergillus were less abundant. Airborne mannitol-positive staphylococci were recorded at low concentrations, ranging from 6 to 11 CFU m−3 on average. According to the taxonomic analysis, Staphylococcus aureus isolates were the most abundant in both schools, followed by S. xylosus, S. haemolyticus and S. saprophyticus. The antibiograms indicated that resistance to erythromycin was common in 62.5% of the isolated staphylococcal strains. Levofloxacin and gentamicin were the most effective antibiotics. No multidrug-resistant strains were identified.

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“…The impact of aerosol particles on the atmosphere and climate as well as on environmental and human health has resulted in more research being conducted on environmental projects ( Šantl-Temkiv et al, 2020 ; Górny, 2020 ). Pathogens have been discovered in the atmosphere since the last century, and bioaerosol research has recently been focused on microorganisms ( Adams et al, 2013 ; Nazaroff, 2014 ; Park et al, 2016 ; Cox et al, 2020 ; Kallawicha, Chao & Kotchasatan, 2019 ; Šantl Temkiv et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Detection and monitoring of insect traces in bioaerosols

Pumkaeo

Takahashi

Iwahashi

2021

PeerJ

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Studies on bioaerosols have primarily focused on their chemical and biological compositions and their impact on public health and the ecosystem. However, most bioaerosol studies have only focused on viruses, bacteria, fungi, and pollen. To assess the diversity and composition of airborne insect material in particulate matter (PM) for the first time, we attempted to detect DNA traces of insect origin in dust samples collected over a two-year period. These samples were systematically collected at one-month intervals and categorized into two groups, PM2.5 and PM10, based on the aerodynamic diameter of the aerosol particles. Cytochrome-c oxidase I (COI) was the barcoding region used to identify the origins of the extracted DNA. The airborne insect community in these samples was analyzed using the Illumina MiSeq platform. The most abundant insect sequences belonged to the order Hemiptera (true bugs), whereas order Diptera were also detected in both PM2.5 and PM10 samples. Additionally, we inferred the presence of particulates of insect origin, such as brochosomes and integument particles, using scanning electron microscopy (SEM). This provided additional confirmation of the molecular results. In this study, we demonstrated the benefits of detection and monitoring of insect information in bioaerosols for understanding the source and composition. Our results suggest that the PM2.5 and PM10 groups are rich in insect diversity. Lastly, the development of databases can improve the identification accuracy of the analytical results.

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Bioaerosol levels and the indoor air quality of laboratories in Bangkok metropolis

Cited by 22 publications

References 41 publications

Microbial diversity of bioaerosol inside sports facilities and antibiotic resistance of isolated Staphylococcus spp.

Microbial diversity of bioaerosol inside sports facilities and antibiotic resistance of isolated Staphylococcus spp.

Diversity of Bioaerosols in Selected Rooms of Two Schools and Antibiotic Resistance of Isolated Staphylococcal Strains (Bydgoszcz, Poland): A Case Study

Detection and monitoring of insect traces in bioaerosols

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