Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo

Yan, Peng; Li, Ying Jian; Zhao, Hui; Zheng, Jun; Xu, Huaizhe; Wei, Gang; Jin, Hao; Cui, Fu De

doi:10.1016/s0378-5173(02)00486-6

Cited by 618 publications

(279 citation statements)

References 20 publications

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“…The presence of higher concentrations of polyelectrolytes probably shifts the ionic equilibrium between insulin and polyelectrolytes towards the associated complexes. Similar conclusion was also drawn from insulinloaded chitosan/TPP nanoparticles [29].…”

Section: Resultssupporting

confidence: 82%

Development and characterization of new insulin containing polysaccharide nanoparticles

Sarmento

Ribeiro

Veiga

et al. 2006

Colloids and Surfaces B: Biointerfaces

226

140

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A nanoparticle insulin delivery system was prepared by complexation of dextran sulfate and chitosan in aqueous solution. Parameters of the formulation such as the final mass of polysaccharides, the mass ratio of the two polysaccharides, pH of polysaccharides solution, and insulin theorical loading were identified as the modulating factors of nanoparticle physical properties. Particles with a mean diameter of 500 nm and a zeta potential of approximately −15 mV were produced under optimal conditions of DS:chitosan mass ratio of 1.5:1 at pH 4.8. Nanoparticles showed spherical shape, uniform size and good shelf-life stability. Polysaccharides complexation was confirmed by differential scanning calorimetry and Fourier transformed infra-red spectroscopy. An association efficiency of 85% was obtained. Insulin release at pH below 5.2 was almost prevented up to 24 h and at pH 6.8 the release was characterized by a controlled profile. This suggests that release of insulin is ruled by a dissociation mechanism and DS/chitosan nanoparticles are pH-sensitive delivery systems. Furthermore, the released insulin entirely maintained its immunogenic bioactivity evaluated by ELISA, confirming that this new formulation shows promising properties towards the development of an oral delivery system for insulin.

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Section: Resultssupporting

confidence: 82%

Development and characterization of new insulin containing polysaccharide nanoparticles

Sarmento

Ribeiro

Veiga

et al. 2006

Colloids and Surfaces B: Biointerfaces

226

140

View full text Add to dashboard Cite

show abstract

“…The ratio between CS and TPP is critical in controlling the size as well as size distribution of NPs thereby affecting their biological performance (Pan et al 2002). Different concentrations of TPP were used to find the optimum CS-TPP ratio so as to obtain nanoparticles with narrow size distribution, before the FA encapsulation.…”

Section: Resultsmentioning

confidence: 99%

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

et al. 2015

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Ferulic acid (FA) is a widely distributed hydroxycinnamic acid found in various cereals and fruits exhibiting potent antioxidant and anticancer activities. However, due to low solubility and permeability, its availability to biological systems is limited. Non-toxic chitosan-tripolyphosphate pentasodium (CS-TPP) nanoparticles (NPs) are used to load sparingly soluble molecules and drugs, increasing their bioavailability. In the present work, we have encapsulated FA into the CS-TPP NPs to increase its potential as a therapeutic agent. Different concentrations of FA were tested to obtain optimum sized FA-loaded CS-TPP nanoparticles (FA/CS-TPP NPs) by ionic gelation method. Nanoparticles were characterized by scanning electron microscopy, Fourier transformation infrared spectroscopy (FTIR), thermogravimetric analyses and evaluated for their anticancer activity against ME-180 human cervical cancer cell lines. The FTIR spectra confirmed the encapsulation of FA and thermal analysis depicted its degradation profile. A concentration-dependent relationship between FA encapsulation efficiency and FA/ CS-TPP NPs diameter was observed. Smooth and spherical FA-loaded cytocompatible nanoparticles with an average diameter of 125 nm were obtained at 40 lM FA conc. The cytotoxicity of 40 lM FA/CS-TPP NPs against ME-180 cervical cancer cell lines was found to be higher as compared to 40 lM native FA. Apoptotic morphological changes as cytoplasmic remnants and damaged wrinkled cells in ME-180 cells were visualized using scanning electron microscopic and fluorescent microscopic techniques. Data concluded that chitosan enveloped FA nanoparticles could be exploited as an excellent therapeutic drug against cancer cells proliferation.

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“…Chitosan nanoparticles have been extensively studied as drug delivery system in recent years (Hua et al, 2011;Jana et al, 2013;Ali et al, 2014). At present, the most common methods for the preparation of chitosan particles were covalent crosslinking (Zheng et al, 2011;Almalik et al, 2013), ion crosslinking (Pan et al, 2002;Sang et al, 2013), sedimentation (Mao et al, 2001) and self-assembly (Baek et al, 2008). However, some concerns have been raised regarding to the safety of chitosan nanoparticles due to the use of toxic crosslinking reagent such as glutaraldehyde, aldehydes and glyoxal, which can inactivate macromolecule drugs (Fürst and Banerjee, 2005;Gupta & Jabrail, 2006;Ajit et al, 2007) and restrict their wide application.…”

Section: Introductionmentioning

confidence: 99%

Development of drug-loaded chitosan–vanillin nanoparticles and its cytotoxicity against HT-29 cells

Wang

Yang

et al. 2014

Drug Delivery

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Chitosan as a natural polysaccharide derived from chitin of arthropods like shrimp and crab, attracts much interest due to its inherent properties, especially for application in biomedical materials. Presently, biodegradable and biocompatible chitosan nanoparticles are attractive for drug delivery. However, some physicochemical characteristics of chitosan nanoparticles still need to be further improved in practice. In this work, chitosan nanoparticles were produced by crosslinking chitosan with 3-methoxy-4-hydroxybenzaldehyde (vanillin) through a Schiff reaction. Chitosan nanoparticles were 200-250 nm in diameter with smooth surface and were negatively charged with a zeta potential of À 17.4 mV in neutral solution. Efficient drug loading and drug encapsulation were achieved using 5-fluorouracil as a model of hydrophilic drug. Drug release from the nanoparticles was constant and controllable. The in vitro cytotoxicity against HT-29 cells and cellular uptake of the chitosan nanoparticles were evaluated by methyl thiazolyl tetrazolium method, confocal laser scanning microscope and flow cytometer, respectively. The results indicate that the chitosan nanoparticles crosslinked with vanillin are a promising vehicle for the delivery of anticancer drugs.

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Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo

Cited by 618 publications

References 20 publications

Development and characterization of new insulin containing polysaccharide nanoparticles

Development and characterization of new insulin containing polysaccharide nanoparticles

Characterization and anticancer potential of ferulic acid-loaded chitosan nanoparticles against ME-180 human cervical cancer cell lines

Development of drug-loaded chitosan–vanillin nanoparticles and its cytotoxicity against HT-29 cells

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