Bioactivity of flavonoid compounds from Poncirus trifoliata L. (Family: Rutaceae) against the dengue vector, Aedes aegypti L. (Diptera: Culicidae)

Abstract

The bioactivity of four flavonoid compounds, namely poncirin, rhoifolin, naringin and marmesin, from Poncirus trifoliata was studied against the Aedes aegypti. Larvicidal assays were conducted to evaluate the 24 h LC(50) and LC(90) values of the flavonoid compounds. The lethal concentration (LC(50) and LC(90)) values ranged from 0.082 to 0.122 mg/l and 0.152 to 0.223 mg/l, respectively. The result of ovicidal test suggests that the ovicidal activity of the flavonoid compounds was influenced by the concentration of flavonoid compounds and age of the eggs. The result of oviposition test showed that the four flavonoid compounds exhibited oviposition-deterrent activity against gravid female mosquitoes. Oviposition decreased with an increase in concentration of flavonoid compounds. A laboratory test was carried out to evaluate protection period and percentage of repellency of four compounds diluted in ethanol (10 mg/l). The compound rhoifolin provided maximum 365.0 +/- 12.0 min protection and also 100.0% +/- 0.0 repellency against mosquito bite followed by poncirin, marmesin and naringin. None of the 25 volunteers of either sex exposed to 10% (w/v) flavonoid compounds (4-h patch test) showed a positive skin irritant reaction. All of the tested compounds proved to have various activities against different life stages of A. aegypti. Therefore, flavonoid compounds from P. trifoliata can be a potential candidate for use in the development of commercial mosquitocidal products that may be an alternative to conventional synthetic chemicals, particularly in integrated vector control applications.

Larvicidal and irritant activities of hexane leaf extracts of Citrus sinensis against dengue vector Aedes aegypti L.

“…Several reports are available regarding the repellent properties of essential oils against adult mosquitoes [18][19][20] ; however very few works have been reported proving the irritability potential of leaf extracts and practically no work with Citrus leaf extracts. Our studies showed the significant irritancy potential of hexane extracts of Citrus leaves against the adults of Ae.…”

A novel herbal formulation against dengue vector mosquitoes Aedes aegypti and Aedes albopictus

“…Zebitz (1984Zebitz ( , 1986 reported that the A. indica seed kernel extract showed ovicidal and oviposition deterrent against A. aegypti, and Mohsen et al (1995) reported similar activity against A. albopictus. Rajkumar and Jebanesan (2008) reported that the isolated compounds from Ponicrus trifoliata showed remarkable ovicidal and oviposition deterrent activity against A. aegypti. The effect on nontarget organisms revealed that PON-NEEM was harmless to predatory fish G. affinis and predatory insect D. indicus.…”

Chemical Constituents and Biological Activities of <i>Zanthoxylum limonella</i> (Rutaceae): A Review

“…Phytochemical survey of this family reveals alkaloids, coumarins, flavonoids, limonoids, and volatile oils [4]. Many of these compounds have been associated to different biological activities, for example, antimicrobial [3,5], antidiarrhoeal [6], anticholinesterasic [7], antileishmanial [8], antiprotozoal [9], larvicidal [10,11], and antioxidant activities [12,13]. In family Rutaceae, the genus Zanthoxylum has been able to provide a variety of secondary metabolites with interesting phytochemical and biological activities [14].…”