Bioactivity of crude fucoidan extracted from Sargassum ilicifolium (Turner) C. Agardh

Abstract

Fucoidan derived from brown algae has been shown to exhibit antitumor and antioxidant effects, so research on sulfated polysaccharides is increasing. The purpose of this study was to evaluate the characteristics and biological activity of fucoidan that was extracted at two temperatures (65 and 80 °C) from Sargassum ilicifolium (Turner) C. Agardh from five regions of Taiwan. The data show that there are significant differences in the yield, sulfate and total sugar content of Sargassum ilicifolium (Turner) C. Agardh grown in different locations in the same sea area. HPLC was used to determine the monosaccharide compositions of the fucoidan, which contains fucose, mannose, mannose, glucose and galactose and have a low molecular weight of less than 5 kDa, and then we will select the algae collected in Fugang, Taitung, for further biological activity research. The sampled Sargassum ilicifolium (Turner) C. Agardh at all five locations has a good polyphenol content, and it shows great DPPH radical scavenging activity, ABTS radical scavenging activity, Ferrous ion-chelating activity and Reducing power. The Sargassum ilicifolium (Turner) C. Agardh that was collected from Taitung Fugang is not toxic to L929 normal cells, but for A549 cancer cells and HCT116 cancer cells, it is known from the results that it has good cytotoxicity for A549 cancer cells. Thus, this study found that the Sargassum ilicifolium (Turner) C. Agardh that was collected from Taitung Fugang has significant antioxidant and anticancer properties.

Potential of Fucoidan From Myriogloea Major Asensi as Antiviral Against Herpes Simplex Type 1 and 2 and Bovine Coronavirus

“…The antioxidant ranges described here for fucoidan extracts from Myriogloea major are similar to those observed in other fucoidan extracts described as antioxidant agents [32,33]. The damage induced by free radicals during oxidative stress contributes to the pathogenesis of neurodegenerative, cardiovascular, in ammatory, and oncological diseases [5,6].…”

“…Despite the scavenging activity of E1 increased by up to ~ 50% as the concentration of fucoidan extract from M. major increased by 1000 µg/ml, the CC 50 value of E1 was > 200 µg/ml in Vero cells, therefore the biological activity should be considered within these ranges. Scavenger activity at 100 µg/ml was approximately 20%, similar to that of crude fucoidan extracted from Sargassum ilicifolium (Turner) C. Agardh [33] and this percentage is a not depreciable scavenger activity.…”

“…Fucoidans are promising molecules to use as natural replacements for antiviral drugs. In this context, the biological properties of fucoidan extracts from several seaweed species were recently evaluated [33,39,59].…”

Cystoseira myrica: from beach-cast seaweed to fucoidan with antioxidant and anticoagulant capacity

“…Fucoidan was obtained with a ratio of 3.07% and contains 3.25% of uronic acid and 35.12% of fucose (Table 1). This fucoidan yield was higher than that extracted from brown seaweed (Lakshmanasenthil et al, 2014;Fauziee et al, 2021;Tsou et al, 2022). For instance, fucoidan was extracted from brown seaweed T. ornata with a yield of 1.8% (Lakshmanasenthil et al, 2014).…”

“…Extraction method, seaweed harvesting season, location, and level of maturation may all influence fucoidan yield and chemical composition (Rani et al, 2017;Baba et al, 2018). Recently, Tsou et al, has extracted fucoidan samples at two temperatures (65 and 80°C) from Sargassum ilicifolium collected from five different locations in southern Taiwan and confirmed that location and temperature of extraction can significantly influence the yield (1.47%-2,73%) and the chemical composition of fucoidan (Tsou et al, 2022). The amount of sulfate and the position of sulfation have been reported to influence the bioactivity of fucoidan as well as the amount of fucose (Jayawardena et al, 2022).…”