Bioactivity of Calcium Aluminate Endodontic Cement

Oliveira, I. R.; Andrade, Talita Luana de; Jacobovitz, Marcos; Pandolfelli, V. C.

doi:10.1016/j.joen.2013.01.013

Cited by 41 publications

(43 citation statements)

References 24 publications

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“…Calcium-aluminate in contact with a wet environment leads to the formation of the AH3 (Al 2 O 3 .3H 2 O) and C3AH6 (3CaO.Al 2 O 3 .6H 2 O) phases, associated with the release of low amounts of Ca ++ , which maintains pH levels similar to those of human body tissues, 17 and leads to the formation of a carbonated apatite phase. 34 The biocompatibility of this material, reported previously, 16,18 seems to be related to the slightly alkaline pH level (about 8.0) achieved after its application to the connective tissues. 17,19 According to the results, the pulp cells seeded onto the BM presented faster proliferative capability at the initial periods, in comparison with those of the NC group.…”

Section: Discussionmentioning

confidence: 54%

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

et al. 2016

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The development of biomaterials capable of driving dental pulp stem cell differentiation into odontoblast-like cells able to secrete reparative dentin is the goal of current conservative dentistry. In the present investigation, a biomembrane (BM) composed of a chitosan/collagen matrix embedded with calcium-aluminate microparticles was tested. The BM was produced by mixing collagen gel with a chitosan solution (2:1), and then adding bioactive calcium-aluminate cement as the mineral phase. An inert material (polystyrene) was used as the negative control. Human dental pulp cells were seeded onto the surface of certain materials, and the cytocompatibility was evaluated by cell proliferation and cell morphology, assessed after 1, 7, 14 and 28 days in culture. The odontoblastic differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, total protein production, gene expression of DMP-1/DSPP and mineralized nodule deposition. The pulp cells were able to attach onto the BM surface and spread, displaying a faster proliferative rate at initial periods than that of the control cells. The BM also acted on the cells to induce more intense ALP activity, protein production at 14 days, and higher gene expression of DSPP and DMP-1 at 28 days, leading to the deposition of about five times more mineralized matrix than the cells in the control group. Therefore, the experimental biomembrane induced the differentiation of pulp cells into odontoblast-like cells featuring a highly secretory phenotype. This innovative bioactive material can drive other protocols for dental pulp exposure treatment by inducing the regeneration of dentin tissue mediated by resident cells.

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Section: Discussionmentioning

confidence: 54%

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

et al. 2016

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“…Silicate ions containing silanol groups are supposed to be released from the bioactive glass and adsorbed on the substrate surface to induce apatite formation 24 . The silicate phase (Ca 2 SiO 4 ) favors the hydroxyapatite generation in SBF medium, which can also stimulate the deposition of mineralized tissue on the biomaterial surface and induce further integration of the latter with the surrounding in vivo components 25,26 . However, there is another method in which the nucleant agent is a sodium silicate solution (SS).…”

Section: Compositionsmentioning

confidence: 99%

Improving the Radiopacity of Calcium Aluminate Cement Based Blends

et al. 2018

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The radiopacity of CAC-based compositions containing additives (ZnO, Ag and Bi 2 O 3 ) was evaluated by optical density measurements, cold crushing strength, apparent porosity, pore size distribution, in vitro apatite-formation ability (bioactivity) and cell viability. The bioactivity was assessed via pH and calcium concentration measurements in simulated body fluid (SBF) for samples previously treated or not with sodium silicate (SS) solution. The surface of the samples after treatment was analyzed by scanning electron microscopy, energy dispersive X-ray analysis and confocal Raman spectroscopy. Cell viability was evaluated by MTT and alkaline phosphatase (ALP) activity. Radiopacity results confirmed that compositions containing ZnO-Ag (25:1 wt%) and ZnO-Bi 2 O 3 (15:10 wt%) presented suitable values of radiopacity (> 3 mmAl). The identification of calcium and mainly phosphorus by EDX, highlighted the generation of an apatite-like layer on the compositions' surface after immersion in SBF or SBF/SS. CAC-blend containing 25 wt% ZnO, ZnO-Ag and ZnO-Bi 2 O 3 presented slightly lower cell viability compared to the positive control.

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“…As the hydration reaction progresses, the hydration products fill these gaps and the porosity decreases. However the conversion process in which the metastable hydrated compounds, CAH 10 , C 2 AH 8 and AH 3 is transformed to the stable C 3 AH 6 and γ-AH 3 at the relatively high temperature of curing medium (37 °C) especially at later curing ages will increase porosity and results in a highly porous system [34][35][36] , Although pores have a significant effect on the mechanical properties of the cement but it also affects its physical properties and, subsequently, its behavior within its environment. Porosity has been shown to act as reservoir for precipitated HA phase during the hydration reaction which have an impact upon bio-remineralization 36,38 .…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Evaluation of Calcium Aluminate/Calcium Phosphate Based Bio-Cements as Root-End Filling Material

Radwan¹,

El-Hamid²,

Abo-Almaged³

et al. 2017

Orient. J. Chem

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Nano-particles of pure single phase calcium aluminate (CA) were prepared via solid state reaction at high temperature. An ultra-pure nano-size hydroxyapatite (HA) prepared by sol-gel method was used to formulate a composite of 1:1 CA: HA. Setting times, micro-hardness, bulk density, calcium ion and phosphorus ion concentration, XRD, FTIR and scanning electron microscopy (SEM) were investigated. The results showed that the substitution of 50% by weight of pure calcium aluminate cement (CA) by nano-size hydroxyapatite (HA) has a retardation effect on setting process. The use of artificial saliva solution as hydration medium enhances the hydration process of both synthesized calcium aluminate based materials due to the formation of more hydrated compounds. Curing of calcium aluminate cement in hot and humid conditions, resulting in hardness loss and high porosity due to the conversion process, in which the compounds CAH 10 and C 2 AH 8 (hexagonal crystals) are converted into the more stable compound C 3 AH 6 (cubic crystals). The SEM studies indicated that the presence of nano-particles of hydroxyapatite in CA/ HA composite facilitated the precipitation of hydroxyapatite-like active layer, suggesting that pure CA cement has some distinctive physico-mechanical properties make it suitable for use as root end filling materials in dental applications.

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Bioactivity of Calcium Aluminate Endodontic Cement

Cited by 41 publications

References 24 publications

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

Chitosan-collagen biomembrane embedded with calcium-aluminate enhances dentinogenic potential of pulp cells

Improving the Radiopacity of Calcium Aluminate Cement Based Blends

Evaluation of Calcium Aluminate/Calcium Phosphate Based Bio-Cements as Root-End Filling Material

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