Bioactivity of Biomass and Crude Exopolysaccharides Obtained by Controlled Submerged Cultivation of Medicinal Mushroom Trametes versicolor

Abstract

The aim of this study is to characterize the bioactivity of mycelial biomass and crude exopolysaccharides (EPS) produced by Trametes versicolor NBIMCC 8939 and to reveal its nutraceutical potential. The EPS (1.58 g/L) were isolated from a culture broth. The macrofungal biomass was rich in protein, insoluble dietary fibers and glucans. The amino acid composition of the biomass was analyzed and 18 amino acids were detected. Three mycelial biomass extracts were prepared and the highest total polyphenol content (16.11 ± 0.14 mg GAE/g DW) and the total flavonoid content (5.15 ± 0.03 mg QE/g DW) were found in the water extract. The results indicated that the obtained EPS were heteropolysaccharides with glucose as the main building monosaccharide and minor amounts of mannose, xylose, galactose, fucose and glucuronic acid. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the complex structure of the crude EPS. Five probiotic lactic acid bacteria strains were used for the determination of the prebiotic effect of the crude EPS. The anti-inflammatory potential was tested in vitro using cell line HT-29. The significant decrease of IL-1 and IL-8 and increase of TGF-beta expression revealed anti-inflammatory potential of the crude exopolysaccharides from T. versicolor.

Bioactive Metabolites from the Fruiting Body and Mycelia of Newly-Isolated Oyster Mushroom and Their Effect on Smooth Muscle Contractile Activity

“…The biomass had less potential than the fruiting body ( Table 2 ), and this trend was confirmed by the performed statistical analysis of the data. This is consistent with the data reported for the medicinal mushroom Trametes versicolor [ 28 , 42 ]. According to the two antiradical assays (DPPH and ABTS), the values for mycelial biomass activity were below the limit of quantification.…”

“…The ability of the extracts to donate an electron and scavenge 2,2-diphenil-1-picrylhydrazyl (DPPH) radicals was determined by the method described by Angelova et al [ 28 ]. The DPPH radical scavenging activity is presented as TEAC (Trolox equivalent antioxidant capacity)—a function of the concentration of Trolox—and is defined as the concentration of Trolox with equivalent antioxidant activity expressed as μM TE/g DW.…”

“…The radical scavenging activity of the extracts against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ) was estimated according to the method described by Angelova et al [ 28 ], and the results are expressed as a TEAC value (μM TE/g DW).…”

“…The FRAP assay was carried out according to the procedure of Angelova et al [ 28 ]. The FRAP reagent was fresh, prepared daily, and was warmed to 37 °C prior to use.…”

“…The CUPRAC assay was carried out according to the procedure described by Angelova et al [ 28 ]. Trolox was used as a standard, and the results are expressed as μM TE/g DW (TEAC).…”