Bioactive Metabolites from the Fruiting Body and Mycelia of Newly-Isolated Oyster Mushroom and Their Effect on Smooth Muscle Contractile Activity

Brazkova, Mariya; Angelova, Galena; Mihaylova, Dasha; Stefanova, Petya; Pencheva, Mina; Gledacheva, Vera; Stefanova, Irina; Krastanov, Albert

doi:10.3390/foods11243983

Cited by 6 publications

(3 citation statements)

References 48 publications

(56 reference statements)

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“…The difference is the extraction time, this author used 24 hours while in these studies the extraction was carried out for 2 hours, suggesting that time is a limiting factor for the extraction of compounds with ferric reduction capacity. Regarding the FRAP results of mycelia extracts, these values are higher than those observed in ethanolic extracts of P. ostreatus (3.27 µM TEAC/g extract) [39] and similar to those observed by Petrovic et al [40] in Laetiporus sulphureus (17.9-29.6 mM TEAC/g extract) in aqueous extractions.…”

Section: Evaluation Of Antioxidant Activity Of Edible Mushroom Ethano...supporting

confidence: 87%

Comparative Evaluation of Antioxidant Activities in Mycelia, Fruiting Bodies and Spent Mushroom Substrate of Edible Mushrooms

Lavega¹,

Grifoll²,

Siles-Sánchez

et al. 2023

Preprint

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Antioxidant activities of 13 commonly cultivated mushrooms in Spain were evaluated using total phenolic content (TPC), DPPH free radical scavenging (DPPH) assay, ABTS free radical scavenging (ABTS) assay, ferric reducing antioxidant power (FRAP) assay and cellular antioxidant activity (CAA) in Caco-2 cell line. Within a mushroom fruiting bodies, Agaricus bisporus var subrufescens possessed the highest TPC and antioxidant capacity in both chemical and cellular assays. Furthermore, the mycelia extracts with the best antioxidant potential in chemical assays were A. bisporus (white), A. bisporus var brunnescens (Portobello) and Hericium erinaceus. Caco-2 treatment with mycelia extracts revealed the absence of activity of any extract in the conditions assayed. In addition, the spent mushroom substrate from Agrocybe aegerita, H. erinaceus and Pholiota nameko are those with the highest polyphenol content and antioxidant capacity both by chemical methods and in vitro models. Results showed that there is no correlation between the polyphenols content, the chemical antioxidant assays and the corresponding values obtained in cellular models. Nevertheless, this study contributes to the rise of data relatively to the species consumed as fresh mushrooms and the possibility of their consumption as a source of antioxidant food.

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Section: Evaluation Of Antioxidant Activity Of Edible Mushroom Ethano...supporting

confidence: 87%

Comparative Evaluation of Antioxidant Activities in Mycelia, Fruiting Bodies and Spent Mushroom Substrate of Edible Mushrooms

Lavega¹,

Grifoll²,

Siles-Sánchez

et al. 2023

Preprint

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show abstract

“…[50][51][52] However, despite its pathogenic nature, research has revealed that A. niger's ability to spread quickly is associated with plants, and there is no information regarding its host specificity. [57][58][59][60] Fungi isolated from medicinal plants are known to have good bioactivity. This indicates that the A. niger found in Sungkai leaves should be capable of producing secondary metabolites that are also present in its host plant.…”

Section: Molecular Identification Of Endophytic Fungimentioning

confidence: 99%

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from The Leaves of Sungkai (Peronema canescens)

2023

TJNPR

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Sungkai (Peronema canescens) is a medicinal plant widely used in Indonesia. Its leaves are believed to have the potential to treat fever and boost the immune system. This study investigated the antioxidant and antibacterial activity of endophytic fungi extracts obtained from Sungkai leaves. This will provide basic information for developing the potential of natural ingredients as antioxidants and antibacterials. Endophytic fungi residing in Sungkai leaves were identified morphologically. The antioxidant test was completed using DPPH, while the antibacterial activity was tested using the paper disk diffusion method. Most potential endophytic fungal isolates were identified through molecular identification, and the isolation of a bioactive compound was achieved using column chromatography. The structure of the compound was determined using 1D and 2D NMR spectroscopy methods. Four endophytic fungi isolates were observed in the Sungkai leaves (code RND1–RND4). RND3 demonstrated very strong antioxidant activity (IC50 < 20 µg/mL) and strong antibacterial activity. Based on the molecular test, RND3 was identified as Aspergillus niger. The pure compound isolated from RND3 revealed weak and strong antioxidant (IC50 > 500 µg/mL) and antibacterial (MIC ≤ 64 µg/mL) properties, respectively. Spectroscopic analysis showed that compound 1 was 3-benzyl-2,6-dihydroxy-1,4,11,13-tetramethyl-5- methylene-12,15-dioxo-14-oxabicycloheptadeca-8,16-diene-7-carboxylic acid. Through further research, this study can be used as a basis for the development of compounds as raw materials for drugs.

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“…The dry mycelium coarse powder was extracted for its metabolites using ethanol and chloroform separately. 30,31 In 50ml of solvent 10g of mycelium powder were added and left on a rotary shaker at 150 rpm for 48 hrs. 32 The extracts were filtered after 48 hrs and the solvent was allowed to evaporate thus leaving behind a thick paste which was stored in glass vials at 4°C until further use.…”

Section: Metabolite Extraction and Fermentationmentioning

confidence: 99%

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

Patankar¹,

Reshi²

2023

J. Pure Appl. Microbiol.

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In the current study, fungi from the mangrove ecosystem of Mumbai were isolated and their metabolites were screened for antibacterial potential. Two weeks old broth and mycelium were extracted using chloroform and methanol. Antibacterial property of solvent extracts was evaluated at various concentrations (2 – 10 µg/ml) against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumonia and Escherichia coli, by well diffusion method. Fungi isolated were identified as Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Cylindrocladium scoparium and Colletotrichum wuxiense. Results revealed that broth solvent extracts of isolates inhibited the growth of all gram-positive test bacteria, chloroform broth extract of Cylindrocladium scoparium, Colletotrichum wuxiense and ethanolic broth extract of Aspergillus flavus, Trichoderma harzianum exhibited antibacterial potential against gram negative test organisms. Chloroform and ethanol mycelium extracts of Trichoderma harzianum and Aspergillus flavus, respectively, exhibited 100% growth inhibition potential against all test organisms. The current investigation endorses the potent secondary metabolism of the identified isolates and their potential to synthesise antibacterial compounds.

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Bioactive Metabolites from the Fruiting Body and Mycelia of Newly-Isolated Oyster Mushroom and Their Effect on Smooth Muscle Contractile Activity

Cited by 6 publications

References 48 publications

Comparative Evaluation of Antioxidant Activities in Mycelia, Fruiting Bodies and Spent Mushroom Substrate of Edible Mushrooms

Comparative Evaluation of Antioxidant Activities in Mycelia, Fruiting Bodies and Spent Mushroom Substrate of Edible Mushrooms

Antioxidant and Antibacterial Activity of Endophytic Fungi Isolated from The Leaves of Sungkai (Peronema canescens)

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

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