One of the insects that cause a lot of damage are termites. Forms of damage that can be caused by termites include damage to building components that made of wood and the contents of the building in the form of furniture, books, cloth, and even securities. One example of a termite species that is dangerous as a pest is Coptotermes curvignathus. Mountain soursop seeds have bioactive compounds that are very cytotoxic, so they have potential as insecticides. This research Used a Completely Randomized Design (CRD) consisting of controls and 5 treatments (P1) 20%, (P2) 40%, (P3) 60%, (P4) 80% (P5) 100% mountain soursop seed extract. The parameters observed were retention, termite mortality, and weight reduction in the test sample. Mountain soursop seed extract is effective as an insecticide against subterranean termites. The concentration of mountain soursop seed extract 40% (P2) causes termite mortality of 100% and a decrease in sample weight by 0.43%. Mountain soursop seed extract has the potential to be developed as an anti-termite species Coptotermes curvignathus Holmgren. The retention value produced in the test sample based on SNI 03-5010.1-1999 shows that the mountain soursop seed extract with a concentration of 20% (P1) meets the standard retention of interior wood use, while extracts with a concentration of 40% (P2) meet the standard of retention of exterior wood use.Keywords: Mountain soursop seeds, Retention, mortality, weight decrease, Subterranean TermiteABSTRAKRayap banyak menimbulkan kerugian seperti rusaknya komponen bangunan yang terbuat dari kayu, beserta isi bangunannya seperti furnitur, kertas, bahan kain dan lain-lain. Salah satu contoh spesies rayap yang berbahaya sebagai hama adalah Coptotermes curvignathus. Biji sirsak gunung memiliki senyawa bioaktif bersifat sitotoksik yang sangat kuat, sehingga memiliki potensi sebagai insektisida. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL), terdiri dari 6 perlakuan yaitu (P1) 20%, (P2) 40%, (P3) 60%, (P4) 80% (P5) 100% dan kontrol. Parameter yang diamati meliputi retensi, mortalitas rayap, dan penurunan berat sampel. Ekstrak biji sirsak gunung efektif sebagai insektisida terhadap rayap tanah. Konsentrasi ekstrak biji sirsak gunung 40% (P2) menyebabkan mortalitas rayap sebesar 100% dan penurunan berat contoh uji sebesar 0,43%. Ekstrak biji sirsak gunung berpotensi dikembangkan sebagai anti rayap tanah jenis Coptotermes curvignathus Holmgren. Hasil penelitian menunjukkan bahwa ekstrak biji sirsak gunung dengan konsentrasi 20% (P1) memenuhi standar retensi penggunaan kayu interior, sementara ekstrak dengan konsentrasi 40% (P2) memenuhi standar retensi penggunaan kayu eksterior.