Bioactivity-guided isolation of immunomodulatory compounds from the fruits of Ligustrum lucidum

Liu, Jia; Liu, Zengyuan; Wang, Lili; He, Hao; Mu, Haibo; Sun, Wenjing; Zhou, Yu; Liu, Yingqiu; Ma, Wuren; Zhang, Weimin; Fu, Mingzhe; Fan, Yunpeng

doi:10.1016/j.jep.2021.114079

Cited by 7 publications

(13 citation statements)

References 52 publications

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“…In vitro data for HT suggest that timing is a fundamental factor in determining the final outcome of HT administration when the inflammatory stimulus is LPS. Experimental results obtained in vitro from LPS-challenged RAW264.7 macrophages demonstrated that 25 µg/mL and 12.5 µg/mL HT cotreatment for 24 h increased IL-1β release, with a mechanism that may depend on IκB-α degradation and NF-κB activation [57]. Instead, 5 µM, 10 µM, 25 µM, 50 µM, and 100 µM HT cotreatment for 6 or 18 h produced no change in IL-1β expression in the same cell line undergoing an analogous stimulation [53].…”

Section: Il-1mentioning

confidence: 93%

“…Instead, in vitro data for HT revealed the activation of a pro-inflammatory molecular network when HT was not used as a pre-treatment before exposing cells to LPS. Cotreatment of LPS-stimulated mouse RAW264.7 macrophages with 50 µg/mL and 25 µg/mL caused an increase in TNF-α production flanked by a HT-dependent NF-κB activation [57], but an exposure to HT shorter than 24 h failed in producing an upregulation of TNFα [53]. A species-dependent effect may be suspected, since in mouse spleen lymphocytes, treatment with 12.5 µg/mL and 6.25 µg/mL HT was per se able to increase TNF-α production [57]; however, analogous evidence was reported for human cells, too.…”

Section: Tnf-αmentioning

confidence: 95%

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Effects of Oleuropein and Hydroxytyrosol on Inflammatory Mediators: Consequences on Inflammaging

Pojero

Aiello

Gervasi

et al. 2022

IJMS

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Aging is associated with a low-grade, systemic inflammatory state defined as “inflammaging”, ruled by the loss of proper regulation of the immune system leading to the accumulation of pro-inflammatory mediators. Such a condition is closely connected to an increased risk of developing chronic diseases. A number of studies demonstrate that olive oil phenolic compound oleuropein and its derivative hydroxytyrosol contribute to modulating tissue inflammation and oxidative stress, thus becoming attractive potential candidates to be used in the context of nutraceutical interventions, in order to ameliorate systemic inflammation in aging subjects. In this review, we aim to summarize the available data about the anti-inflammatory properties of oleuropein and hydroxytyrosol, discussing them in the light of molecular pathways involved in the synthesis and release of inflammatory mediators in inflammaging.

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Section: Il-1mentioning

confidence: 93%

Section: Tnf-αmentioning

confidence: 95%

Effects of Oleuropein and Hydroxytyrosol on Inflammatory Mediators: Consequences on Inflammaging

Pojero

Aiello

Gervasi

et al. 2022

IJMS

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“…The evidence supporting a role for OLE and HT in the regulation of immune cell frequency is minimal. Previous studies on mouse spleen lymphocytes demonstrated that 50 μg/mL HT could augment the percentage of CD3+ mouse lymphocytes, and 50, 25, 12.5, and 6.25 μg/mL HT caused an increase in the CD3+CD4−CD8− T cell subset in mouse after 48 h [ 95 ]. Moreover, 50, 100, and 200 μg/mL OLE promote the expansion of CD4+CD25+FoxP3+ T regulatory cells (Tregs) in both healthy controls and rheumatoid arthritis patients after 24 h [ 75 ].…”

Section: Discussionmentioning

confidence: 99%

“…In another in vitro model, 20–40 μM and 10–40 μM OLE reduce the LPS-triggered secretion of TNF-α and IL-6 by J774A.1 murine macrophages [ 73 ]. On the contrary, 12.5 and 6.25 μg/mL HT stimulated TNF-α secretion by mouse spleen lymphocytes [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis

Pojero

Gervasi

Fiore

et al. 2023

IJMS

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Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18–64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes.

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“…Absorbance at 570 nm was measured after incubation at room temperature for 30 min. The phagocytic index was calculated using the following formula (Liu et al, 2021):…”

Section: Phagocytic Functionmentioning

confidence: 99%

The protective effect and antitumor activity of Aconiti Lateralis Radix Praeparata (Fuzi) polysaccharide on cyclophosphamide-induced immunosuppression in H22 tumor-bearing mice

Liu

et al. 2023

Front. Pharmacol.

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Background:Aconiti Lateralis Radix Praeparata, also known as Fuzi in Chinese, has been used in Traditional Chinese Medicine for more than 2,000 years. In recent years, some traditional herbal compounds containing Fuzi have achieved positive clinical results in tumor treatment. And the polysaccharide isolated from Fuzi has attracted much attention as a potential immunomodulator. However, its immunomodulatory mechanism remains to be further studied. Aim of the study. Fuzi neutral polysaccharide (FNPS) and cyclophosphamide (CTX) were combined to treat Hepatoma 22 (H22) tumor-bearing mice, and its mechanism of ameliorating immunosuppression caused by CTX was studied.Methods: FNPS was isolated and purified. The molecular weight, functional groups, monosaccharide composition, and apparent morphology were characterized by gel permeation chromatography, Fourier transform infrared spectrometer, ion chromatography and scanning electron microscope, respectively. Through the analysis of tumor, immune organs, and serum cytokine levels of H22 tumor-bearing mice, the immunomodulatory effect and the protective effect on immunosuppressive mice induced by CTX was evaluated. And the immunomodulatory activity of FNPS was further verified by macrophage functional experiments.Results: FNPS was composed of rhamnose, arabinose, galactose, glucose, and mannose in a molar ratio of 0.008:0.017:0.018:0.908:0.048. Its molecular weight was 94 kDa. In vivo experiments showed that 200 mg mL−1 FNPS could alleviate the suppression of immune organs and immune cells caused by CTX treatment, enhance the antitumor effect of CTX, increase the serum levels of Th1 immune-related pro-inflammatory cytokines (IL-1β and IL-6), and decrease Th2 immune-related anti-inflammatory cytokine (IL-10) and tumor-related pro-inflammatory cytokine (TNF-α) in the chemotherapy mice. Functional experiments revealed that 25 μg mL−1 FNPS could promote phagocytosis and proliferation of macrophages. When the concentration reached 50 μg mL−1, it enhanced the migration activity.Conclusion: FNPS has the potential to alleviate the immunosuppressive effect of CTX by activating immune cells and promoting inflammation. It could be used as a potential auxiliary medication for liver cancer treatment.

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Bioactivity-guided isolation of immunomodulatory compounds from the fruits of Ligustrum lucidum

Cited by 7 publications

References 52 publications

Effects of Oleuropein and Hydroxytyrosol on Inflammatory Mediators: Consequences on Inflammaging

Effects of Oleuropein and Hydroxytyrosol on Inflammatory Mediators: Consequences on Inflammaging

Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis

The protective effect and antitumor activity of Aconiti Lateralis Radix Praeparata (Fuzi) polysaccharide on cyclophosphamide-induced immunosuppression in H22 tumor-bearing mice

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