Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans

Abstract

Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics.

Antimicrobial and Antioxidant Property of a True Mangrove Rhizophora apiculata Bl.

“…[52,53] A mixture of active compounds from mangroves showed a wide range of pharmacological and biological significance. [54][55][56] R. apiculata extracts are more active against C. albicans and A. fumigatus which warrants future screening for herbal control of Candidiasis, Aspergillosis and UTI related bacterial infections. Hence, the sensitivity of pathogens can be screened out and herbal preparations can be standardised for effective pathogen control.…”

“…Mangroves are known for their efficient activity against microorganisms [52,53] . A mixture of active compounds from mangroves showed a wide range of pharmacological and biological significance [54–56] . R. apiculata extracts are more active against C. albicans and A. fumigatus which warrants future screening for herbal control of Candidiasis, Aspergillosis and UTI related bacterial infections.…”

A Meta-Omics Analysis Unveils the Shift in Microbial Community Structures and Metabolomics Profiles in Mangrove Sediments Treated with a Selective Actinobacterial Isolation Procedure

“…For example, a Streptomyces strain isolated from the top sediment layer of a mangrove in Koringa, India, possessed antimicrobial activity against Candida albicans and Pectinotrichum llanense [ 27 ], while another Streptomyces strain isolated from Indian mangrove sediments was reported to possess promising antioxidant, antimicrobial, and anti-inflammatory agents [ 28 ]. However, descriptions of the biotechnological potential of mangrove microbial communities through the use of genome mining is scarce, and little research has focused on identifying genes involved in the biosynthesis of medically and industrially relevant compounds from these sediments [ 29 , 30 , 31 , 32 ]. Among these genes, polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes are the most commonly used to assess the natural product potential of a community due to their involvement in the biosynthesis of numerous bioactive compounds [ 33 , 34 , 35 ].…”

Investigation of anti‐inflammatory and toxicity effects of mangrove‐derived Streptomyces rochei strain VITGAP173

“…With research interests of actinobacterial metabolites, our previous study explored some of the antifungal metabolites from actinobacteria . This study focuses on investigating the anti‐inflammatory effects of secondary metabolites present in the ethyl acetate extracts of actinobacteria on RAW264.7 macrophages, in vivo oral acute, subacute toxicity and anti‐inflammatory analysis.…”

“…With research interests of actinobacterial metabolites, our previous study explored some of the antifungal metabolites from actinobacteria. 23 This study focuses on investigating the anti-inflammatory effects of secondary metabolites present in the ethyl acetate extracts of actinobacteria on RAW264.7 macrophages, in vivo oral acute, subacute toxicity and antiinflammatory analysis. The metabolic profile of potential isolate was identified, and the inhibitory effects of the extract on proinflammatory mediators such as TNF-α, IL-6, and COX-2 were analyzed in lipopolysaccharide (LPS)-induced RAW264.7 macrophages.…”