Bioactive triterpene glycosides from the fruit of Stauntonia hexaphylla and insights into the molecular mechanism of its inflammatory effects

Vinh, Le Ba; Jang, Hyun‐Jae; Phong, Nguyen Viet; Gao, Dan; Cho, Kyoung Won; Kim, Young Ho; Yang, Seo Young

doi:10.1016/j.bmcl.2019.07.010

Cited by 21 publications

(18 citation statements)

References 36 publications

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“…Natural bioactive compounds, such as phenolics, triterpene saponins, and carbohydrates, are responsible for the pharmaceutical activities of medicinal herbs [ 13 , 14 ]. In an effort to discover new anti-inflammatory agents in medicinal plants, we isolated 15 compounds from a methanol (MeOH) extract of P. tenuifolia roots using bioassay-guided and chromatographic separation methods.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Compounds from Polygala tenuifolia and Their Inhibitory Effects on Lipopolysaccharide-Stimulated Pro-inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells

Vinh

Heo

Phong

et al. 2020

Plants

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The roots of Polygala tenuifolia Wild (Polygalaceae), which is among the most important components of traditional Chinese herbal medicine, have been widely used for over 1000 years to treat a variety of diseases. In the current investigation of secondary metabolites with anti-inflammatory properties from Korean medicinal plants, a phytochemical constituent study led to the isolation of 15 compounds (1–15) from the roots of P. tenuifolia via a combination of chromatographic methods. Their structures were determined by means of spectroscopic data such as nuclear magnetic resonance (NMR), 1D- and 2D-NMR, and liquid chromatography-mass spectrometry (LC-MS). As the obtained results, the isolated compounds were divided into two groups—phenolic glycosides (1–9) and triterpenoid saponins (10–15). The anti-inflammatory effects of crude extracts, fractions, and isolated compounds were investigated on the production of the pro-inflammatory cytokines interleukin (IL)-12 p40, IL-6, and tumour necrosis factor-α in lipopolysaccharide-stimulated bone marrow-derived dendritic cells. The IC50 values, ranging from 0.08 ± 0.01 to 21.05 ± 0.40 μM, indicated potent inhibitory effects of the isolated compounds on the production of all three pro-inflammatory cytokines. In particular, compounds 3–12, 14, and 15 showed promising anti-inflammatory activity. These results suggest that phenolic and triterpenoid saponins from P. tenuifolia may be excellent anti-inflammatory agents.

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Section: Introductionmentioning

confidence: 99%

Bioactive Compounds from Polygala tenuifolia and Their Inhibitory Effects on Lipopolysaccharide-Stimulated Pro-inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells

Vinh

Heo

Phong

et al. 2020

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“…Previous studies have reported that S . hexaphylla is a precious herbal medicine due to its anti-diabetic, anti-inflammatory, and antioxidant effects [ 2 – 6 ]. Cornus officinalis Sieb et Zucc ( C .…”

Section: Introductionmentioning

confidence: 99%

Protective effects of combination of Stauntonia hexaphylla and Cornus officinalis on testosterone-induced benign prostatic hyperplasia through inhibition of 5α- reductase type 2 and induced cell apoptosis

et al. 2020

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Benign prostatic hyperplasia (BPH) is a progressive pathological condition associated with proliferation of prostatic tissues, prostate enlargement, and lower-urinary tract symptoms. However, the mechanism underlying the pathogenesis of BPH is unclear. The aim of this study was to investigate the protective effects of a combination of Stauntonia hexaphylla and Cornus officinalis (SC extract) on a testosterone propionate (TP)-induced BPH model. The effect of SC extract was examined in a TP-induced human prostate adenocarcinoma cell line. Male Sprague-Dawley rats were randomly divided into 5 groups (n = 6) for in vivo experiments. To induce BPH, all rats, except those in the control group, were administered daily with subcutaneous injections of TP (5 mg/kg) and orally treated with appropriate phosphate buffered saline/drugs (finasteride/saw palmetto/SC extract) for 4 consecutive weeks. SC extract significantly downregulated the androgen receptor (AR), prostate specific antigen (PSA), and 5α-reductase type 2 in TP-induced BPH in vitro . In in vivo experiments, SC extract significantly reduced prostate weight, size, serum testosterone, and dihydrotestosterone (DHT) levels. Histologically, SC extract markedly recovered TP-induced abnormalities and reduced prostatic hyperplasia, thereby improving the histo-architecture of TP-induced BPH rats. SC extract also significantly downregulated AR and PSA expression, as assayed using immunoblotting. Immunostaining revealed that SC extract markedly reduced the 5α-reductase type 2 and significantly downregulated the expression of proliferating cell nuclear antigen. In addition, immunoblotting of B-cell lymphoma 2 (Bcl-2) family proteins indicated that SC extract significantly downregulated anti-apoptotic Bcl-2 and markedly upregulated pro-apoptotic B cell lymphoma-associated X (Bax) expression. Furthermore, SC treatment significantly decreased the Bcl-2/Bax ratio, indicating induced prostate cell apoptosis in TP-induced BPH rats. Thus, our findings demonstrated that SC extract protects against BPH by inhibiting 5α-reductase type 2 and inducing prostate cell apoptosis. Therefore, SC extract might be useful in the clinical treatment of BPH.

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“…According to our previous experimental data and previous literature, peak 5 was tentatively identified as anhuienside C [11,12]. Peaks 13,14, and 15 are attributed to the same aglycone ions as that of peak 5; they were tentatively assigned as araliasaponin II, hexaphyloside A, and ciwujianoside C3, respectively, by comparison with known compounds isolated from S. hexaphylla and their families [13][14][15][16][17] Figure S4). The fragmentation of parent ion produced daughter ions at m/z 928.5, 752.5, and 603.2 attributed to the loss of Ara-Glc, Rha, and Glc.…”

Section: Identification and Characterization Of Chemical Constitutionmentioning

confidence: 57%

Discrimination and quality evaluation of fifteen components in Stauntonia hexaphylla leaves from different harvest time by HPLC–PDA–ESI–MS/MS and ELSD coupled with multivariate statistical analysis and anti-inflammatory activity evaluation

et al. 2020

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The leaves of Stauntonia hexaphylla (SHL) are a very popular herbal medicine in Korea because it can be used to treat rheumatic osteoporosis and other diseases. However, owing to the inconsistency in harvesting time and growth years, their quality is uneven, which indirectly negatively affects the safety of this medication. Therefore, the difference of constituents in SHL harvested at different seasons and years were analyzed by high-performance liquid chromatography coupled to photodiode-array and electrospray ionization mass spectrometry detectors (HPLC–PDA–ESI/MS). A total of 15 components were tentatively characterized in samples of SHL, including 5 compounds reported for the first time in this plant. Moreover, the relative content of these constitutions was simultaneously determined by HPLC coupled with evaporative light scattering detection (ELSD). Hierarchical clustering analysis (HCA) and principal component analysis (PCA) revealed that the quality has a certain extent difference in different harvest times, the best harvest time was 3 years old growing in autumn. The same harvest time was also suggested based on the anti-inflammatory evaluation.

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Bioactive triterpene glycosides from the fruit of Stauntonia hexaphylla and insights into the molecular mechanism of its inflammatory effects

Cited by 21 publications

References 36 publications

Bioactive Compounds from Polygala tenuifolia and Their Inhibitory Effects on Lipopolysaccharide-Stimulated Pro-inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells

Bioactive Compounds from Polygala tenuifolia and Their Inhibitory Effects on Lipopolysaccharide-Stimulated Pro-inflammatory Cytokine Production in Bone Marrow-Derived Dendritic Cells

Protective effects of combination of Stauntonia hexaphylla and Cornus officinalis on testosterone-induced benign prostatic hyperplasia through inhibition of 5α- reductase type 2 and induced cell apoptosis

Discrimination and quality evaluation of fifteen components in Stauntonia hexaphylla leaves from different harvest time by HPLC–PDA–ESI–MS/MS and ELSD coupled with multivariate statistical analysis and anti-inflammatory activity evaluation

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