Bioactive structural basis of proteoglycans from Sarcandra glabra based on spectrum-effect relationship

Sun, Xuyang; Zhao, Qianqian; Si, Yu; Li, Kaidong; Zhu, Junkai; Gao, Xiangdong; Liu, Wei

doi:10.1016/j.jep.2020.112941

Cited by 15 publications

(15 citation statements)

References 50 publications

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“…It also contains 16 kinds of amino acids, such as aspartic acid, glutamic acid, leucine and so on, six kinds of which are essential amino acids for human body, as well as trace elements including iron, zinc, calcium, magnesium and so on (Yang B. et al, 2008). In addition, acidic polysaccharide and proteoglycan are also isolated from S. glabra (Liu W. et al, 2017;Sun et al, 2020).…”

Section: Other Compoundsmentioning

confidence: 99%

The Traditional Uses, Phytochemistry and Pharmacology of Sarcandra glabra (Thunb.) Nakai, a Chinese Herb With Potential for Development: Review

et al. 2021

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Sarcandra glabra (Thunb.) Nakai is a folk medicine with a long history in China, which has been applied to treat sore throat, abscess, even tumor and so on. Meanwhile, it is also used as tea in some areas. At present, more than 200 chemical compounds have been isolated and identified from it, such as, sesquiterpenes, flavonoids, phenolic acids, coumarins and so on. Pharmacological studies have already confirmed that the extracts of S. glabra have many effects, such as antibacterial, antiviral, anti-inflammatory, anti-tumor, and anti-thrombocytopenia, especially the effects of anti-tumor and anti-thrombocytopenia are confirmed in clinic. Therefore, this paper systematically summarized the traditional uses, botany, phytochemistry, pharmacology, and toxicity of S. glabra, in order to provide a beneficial reference of its further research.

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Section: Other Compoundsmentioning

confidence: 99%

The Traditional Uses, Phytochemistry and Pharmacology of Sarcandra glabra (Thunb.) Nakai, a Chinese Herb With Potential for Development: Review

et al. 2021

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“…The VIP value is an important index reflecting the ability of independent variables to explain dependent variables. Variables with a VIP value >1.0 contribute significantly to the model and are regarded as an important evaluation index for pharmacodynamics (Sun, Zhao, et al, 2020). Therefore, P18, P10, P11, P5, P14, P21, P28, P25, P6, P22 and P15, with regression coefficients >0, especially P18 and P10 (VIP > 1), were considered as potential anti‐ α ‐glucosidase compounds of C. paliurus leaves in the PLS model.…”

Section: Resultsmentioning

confidence: 99%

α‐Glucosidase inhibitors screening from Cyclocarya paliurus based on spectrum–effect relationship and UPLC–MS/MS

Yan-jun

Guo

et al. 2022

Biomedical Chromatography

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Cyclocarya paliurus is an edible and medicinal plant exhibiting significant hypoglycemic effect. However, its active components are still unclear and need further elucidation. In this research, the active components of the leaves of C. paliurus responsible for the α-glucosidase inhibitory activity were screened and identified based on a spectrum-effect relationship study in combination with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis. The 70% ethanol eluate fraction of the leaves of C. paliurus with the strongest α-glucosidase inhibitory activity was obtained after extraction and purification with macroporous resin.Their chromatographic fingerprints (15 batches) were established by UPLC analysis and 32 common peaks were specified by similarity analysis. Their IC 50 values for α-glucosidase inhibition were measured by an enzymatic reaction. Several multivariate statistical analysis methods including hierarchical cluster analysis, principal component analysis, partial least square analysis and gray relational analysis were applied to explore the spectrum-effect relationship between common peaks and IC 50 values, and the chromatographic peaks making a large contribution to efficacy were screened out. To further elucidate the active components of leaves of C. paliurus, the 70% ethanol eluate fraction was characterized by UPLC-MS/MS analysis, and 10 compounds were identified. This study provides a valuable reference for further research and development of hypoglycemic active components of C. paliurus.

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“…(3), tyrosine (4), uracil (7), adenine (8), inosine (9), vernine (10), 5hydroxymethylfurfural (12), tryptophan (14), and adenosine (15). The similarity values and common peak areas of 20 PRPCA batches are presented in Tables 1 and 2, respectively.…”

Section: Similarity Analysis Of Hplc Fingerprintsmentioning

confidence: 99%

“…Thus, the quality of PRPCA cannot be evaluated comprehensively using the evaluation method recorded in the Chinese pharmacopoeia (2020 Edition). The spectrum-effect relationship (between chemical compositions and efficacy) analysis has been widely applied to explore the active substances in TCM [9].…”

Section: Introductionmentioning

confidence: 99%

Spectrum-effect relationship between HPLC fingerprints and inhibitory activity in MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine

Liu¹,

Pei²,

He³

et al. 2022

Trop. J. Pharm Res

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Purpose: To investigate the spectrum-effect relationship between HPLC fingerprints and the inhibitory effect on MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA).Methods: The fingerprints of 20 PRPCA batches were established using HPLC and their similarities or differences were analyzed using hierarchical cluster analysis (HCA) and principal component analysis (PCA). The inhibitory effects of MUC5AC mucin were evaluated in LPS-treated NCI-H292 cells. The spectrum-effect relationship between common chromatographic peaks and MUC5AC inhibition was established using a partial least squares-discriminant analysis (PLS-DA).Results: Fifteen common chromatographic peaks were identified by analyzing HPLC fingerprints, with uridine, tyrosine, uracil, and inosine found as possible markers to distinguish the PRPCA from different sources. Spectrum-effect relationship analysis showed that the chromatographic peaks 5, 6, 10 (vernine), 12 (5-hydroxymethylfurfural), 14 (tryptophan) and 15 (adenosine) were closely associated with the inhibitory effect on MUC5AC mucin.Conclusion: The spectrum-effect relationship between HPLC fingerprints and the inhibitory effect on MUC5AC mucin of PRPCA was successfully established in the present study. Our findings further reveal the material basis of PRPCA and provide an effective method for its quality control.

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Bioactive structural basis of proteoglycans from Sarcandra glabra based on spectrum-effect relationship

Cited by 15 publications

References 50 publications

The Traditional Uses, Phytochemistry and Pharmacology of Sarcandra glabra (Thunb.) Nakai, a Chinese Herb With Potential for Development: Review

The Traditional Uses, Phytochemistry and Pharmacology of Sarcandra glabra (Thunb.) Nakai, a Chinese Herb With Potential for Development: Review

α‐Glucosidase inhibitors screening from Cyclocarya paliurus based on spectrum–effect relationship and UPLC–MS/MS

Spectrum-effect relationship between HPLC fingerprints and inhibitory activity in MUC5AC mucin of Pinelliae Rhizoma Praeparatum Cum Alumine

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