Bioactive Secondary Metabolites from Terrestrial Streptomyces baarnensis MH4

Nasr, Hamdi; Abdel‐Aziz, Mohamed S.; Radwan, Mohamed O.; Shaaban, Mohamed

doi:10.9734/bjpr/2015/14074

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…In recent years, natural products and their derivatives have attracted great deal of interest as a source for the development of new diverse bioactive compounds due to their high safety profile and cost effectiveness [20,21,22,23,24,25,26]. Among them, pentacyclic triterpenes are the subjects of numerous studies carried out by chemists engaged in organic synthesis and pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Design, Synthesis and Biological Evaluation of Pentacyclic Triterpene Derivatives: Optimization of Anti-ABL Kinase Activity

et al. 2019

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Imatinib, an Abelson (ABL) tyrosine kinase inhibitor, is a lead molecular-targeted drug against chronic myelogenous leukemia (CML). To overcome its resistance and adverse effects, new inhibitors of ABL kinase are needed. Our previous study showed that the benzyl ester of gypsogenin (1c), a pentacyclic triterpene, has anti-ABL kinase and a subsequent anti-CML activity. To optimize its activities, benzyl esters of carefully selected triterpenes (PT1–PT6), from different classes comprising oleanane, ursane and lupane, and new substituted benzyl esters of gypsogenin (GP1–GP5) were synthesized. All of the synthesized compounds were purified and charachterized by different spectroscopic methods. Cytotoxicity of the parent triterpenes and the synthesized compounds against CML cell line K562 was examined; revealing three promising compounds PT5, GP2 and GP5 (IC50 5.46, 4.78 and 3.19 μM, respectively). These compounds were shown to inhibit extracellular signal-regulated kinase (ERK) downstream signaling, and induce apoptosis in K562 cells. Among them, PT5 was identified to have in vitro activity (IC50 = 1.44 μM) against ABL1 kinase, about sixfold of 1c, which was justified by molecular docking. The in vitro activities of GP2 and GP5 are less than PT5, hence they were supposed to possess other more mechanisms of cytotoxicity. In general, our design and derivatizations resulted in enhancing the activity against ABL1 kinase and CML cells.

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Section: Introductionmentioning

confidence: 99%

Design, Synthesis and Biological Evaluation of Pentacyclic Triterpene Derivatives: Optimization of Anti-ABL Kinase Activity

et al. 2019

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“…The VLC fraction, JT2 revealed indolyl-3-acetic acid with a retention time of 28.37 min while the indole derivative with a retention time of 17.03 min was also identified in the crude extract. A previous report [33] isolated indolyl-3-acetic acid (IAA), an indole derivative containing a carboxymethyl substituent from the terrestrial Streptomyces baarnensis, MH4. Activities reported for the indole derivative Includes anti-inflammatory [34], antioxidant, anticancer [35], [36] and antimicrobial properties [37].…”

Section: Discussionmentioning

confidence: 99%

“…Activities reported for the indole derivative Includes anti-inflammatory [34], antioxidant, anticancer [35], [36] and antimicrobial properties [37]. The fraction, JT4, of the VLC fractions revealed indole-3-carbaldehyde with a retention time of 23.54 min previously isolated from a marine sponge, Halichondria species [38] and from the terrestrial Streptomyces baanensis, MH4 [33]. Li et al [38] reported the antifungal activity of Indole-3-carbaldehyde against Mortierella ramannianus.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of secondary metabolites produced by Colletotrichum species, an endophytic fungus on Vernonia amygdalina Del (fam. Asteraceae)

Okoli¹,

Okolo²,

Anyanwu³

et al. 2023

World J. Bio. Pharm. Health Sci.

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The emergence of antibiotic-resistant strains of microorganisms calls for research and the development of strategies to curb this menace. The screening of antimicrobial compounds from endophytic fungi is a promising way to meet the increasing threat of drug-resistant. This study aims at evaluating the antimicrobial activity of the endophytic fungal extract of Colletotrichum sp. from the leaves of Vernonia amygdalina (Del). Endophytic fungal isolation, cultivation, and identification were carried out using standard methods. Subsequently, fermentation and extraction of the secondary metabolites were done on a rice medium. About 1.2g of the fungal extract was subjected to vacuum liquid chromatography (VLC) using gradient-based elution; n-hexane and ethyl acetate followed by dichloromethane and methanol. The high-performance liquid chromatography (HPLC) analysis of the crude extract and VLC fractions were also carried out. Gel permeation chromatography using Sephadex LH-20 was carried out on Fraction JT2. Antimicrobial Assays for fraction JT4 and the Sephadex LH-20 fractions (JT2C – JT2E) were also carried out. The results were analyzed using Statistical Package for Social Sciences. The high-performance liquid chromatography (HPLC) analysis of the crude extract and VLC fractions revealed the presence of the five bioactive metabolites (acropyrone, beauvericin, indole-3-carbaldehyde, indolyl-3-acetic acid, and rocaglamid A). The result of the antimicrobial activity of the VLC fraction, JT4, showed that the fraction exhibited a significant inhibitory effect against Bacillus subtilis, Pseudomonas aeruginosa at 0.25 – 1 mg/ml (IZD) and antifungal activity against Aspergillus niger only at 0.0625 – 1 mg/ml (IZD). The result of the antimicrobial activity of the gel chromatographic fractions revealed a significant effect against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli at 0.0625 – 1 mg/ml and had no inhibitory effect against Bacillus subtilis, Aspergillus niger and Candida albicans. The results of this study show that endophytic fungi on V. amygdalina could be a potential source of bioactive compounds for the development of potential and effective antibiotics.

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“…Streptomyces has largest genus most commonly found in terrestrial habitat, at least 90% actinomycetes isolated from soil have been reported to be Streptomyces spp. such as streptomyces kanamyceticus, streptomyces fradiae, streptomyces griseus, streptomyces antibioticus, streptomyces ambofaciens, streptomyces venezuelae, streptomyces lincolnensis, streptomyces roseosporus and actinoplanin teichomyceticus that produce important antibiotic [12]. Streptomyces are characterized by production of various extra-cellular enzymes and different kind of bio-active secondary metabolites that have great structural and functional diversity used as an antibacterial, antiprotozoal, antifungal, antiviral, antihelminthic, anticholesterol, anticancer, immunosuppressant, pesticides and herbicides [13].…”

Section: Introductionmentioning

confidence: 99%

Soil actinomycetes molecular characterization for secondary metabolites production

Ullah

Muhammad

Jehan

et al. 2021

Journal of advanced Biomedical and Pharmaceutical Sciences

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Antibiotic resistance issue is really unavoidable, increased due to decrease in production of novel secondary metabolites production. Actinomycetes have the potential to produce biological active compounds used as an antibacterial, antiviral, antifungal, antiprotozoal, antihelminthic, anticholesterol, anticancer, immunosuppressant, pesticides and herbicides. Current research study was design to assess actinomycetes for production secondary metabolites. For this purpose different soil samples were collected and with help of dilution method, actinomycetes were grown on enrichment. Actinomycetes were morphologically analysed under microscope and molecularly through PCR and 16S-rRNA gene-amplification. After confirmation, actinomycetes were grown on LB and GMC media have potential to produce bioactive secondary metabolites. Current research study results that actinomycetes have potential to produce bioactive secondary metabolites. LB-media is the best suitable-medium for growth of actinomycetes and bioactive secondarymetabolites production. It is suggested that actinomycetes strains from various environment should be assessed for novel bioactive secondary metabolites.

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Bioactive Secondary Metabolites from Terrestrial Streptomyces baarnensis MH4

Cited by 6 publications

References 22 publications

Design, Synthesis and Biological Evaluation of Pentacyclic Triterpene Derivatives: Optimization of Anti-ABL Kinase Activity

Design, Synthesis and Biological Evaluation of Pentacyclic Triterpene Derivatives: Optimization of Anti-ABL Kinase Activity

Antimicrobial activity of secondary metabolites produced by Colletotrichum species, an endophytic fungus on Vernonia amygdalina Del (fam. Asteraceae)

Soil actinomycetes molecular characterization for secondary metabolites production

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