Soil actinomycetes molecular characterization for secondary metabolites production

Abstract

Antibiotic resistance issue is really unavoidable, increased due to decrease in production of novel secondary metabolites production. Actinomycetes have the potential to produce biological active compounds used as an antibacterial, antiviral, antifungal, antiprotozoal, antihelminthic, anticholesterol, anticancer, immunosuppressant, pesticides and herbicides. Current research study was design to assess actinomycetes for production secondary metabolites. For this purpose different soil samples were collected and with help of dilution method, actinomycetes were grown on enrichment. Actinomycetes were morphologically analysed under microscope and molecularly through PCR and 16S-rRNA gene-amplification. After confirmation, actinomycetes were grown on LB and GMC media have potential to produce bioactive secondary metabolites. Current research study results that actinomycetes have potential to produce bioactive secondary metabolites. LB-media is the best suitable-medium for growth of actinomycetes and bioactive secondarymetabolites production. It is suggested that actinomycetes strains from various environment should be assessed for novel bioactive secondary metabolites.

Biodiversity of Actinomycetes and Their Secondary Metabolites: A Comprehensive Review

“…Actinomycetes produce about two-thirds of all antibiotics available in the market. Bio-active secondary metabolites produced by actinomycetes are used as antibiotics, antifungal, anticholesterol, antiprotozoal, antiviral, anticancer, antihelminth immunosuppressant and as a single cell protein [2]. Actinomycetes are commonly found in natural environment and have an important role in biodegradation of organic matter [3].…”

“…Actinomycetes have prominent value in their diversity, their secondary metabolites have the capability of bioactive molecules which act as antibiotics, enzymes and immunosuppressive and antitumor agents and produced half of the available antibiotics on the market. For the past fifty years, due to huge antibioticproducing sources, efforts have been focused on actinomycetes for the production of novel antibiotics [2,4].…”