Bioactive polymers: Synthesis, release study and antimicrobial properties of polymer bound Ampicillin

Patel, Jignesh B.; Patel, Sanjay V.; Talpada, Navin P.; Patel, Hasmukh S.

doi:10.1002/(sici)1522-9505(19991101)271:1<24::aid-apmc24>3.0.co;2-l

Cited by 32 publications

(24 citation statements)

References 10 publications

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“…The vast majority of such antimicrobial fibers involve polymers in some capacity, either woven into another textile or assembled as nonwoven mats. Often, natural or synthetic polymers are grafted or chemically conjugated to antimicrobial agents such as antibiotics [16], quaternary ammonium moieties [17], phenols [18], and halogenated compounds [19]. However, due to the reaction selectivity of the base polymer, additional or multiple functionalities can be difficult to impart upon such fibers.…”

Section: Introductionsupporting

confidence: 54%

Genetically Engineered Phage Fibers and Coatings for Antibacterial Applications

Mao

Belcher

Vliet

2010

Adv Funct Materials

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Multifunctionality can be imparted to protein-based fibers and coatings via either synthetic or biological approaches. Here, we demonstrate potent antimicrobial functionality of genetically engineered, phage-based fibers and fiber coatings, processed at room temperature. Facile genetic engineering of the M13 virus (bacteriophage) genome leverages the well-known antibacterial properties of silver ions to kill bacteria. Predominant expression of negatively-charged glutamic acid (E3) peptides on the pVIII major coat proteins of M13 bacteriophage (or phage) enables solution-based, electrostatic binding of silver ions and subsequent reduction to metallic silver along the phage length. Antibacterial fibers of micrometer-scale diameters are constructed from such E3-modified phage, via wet-spinning and glutaraldehyde-crosslinking of the E3-modified phage. Silverization of the free-standing fibers is confirmed via energy-dispersive spectroscopy (EDS) and inductively-coupled plasma atomic emission spectroscopy (ICP-AES), showing -0.61,pg/cm of silver on E3-Ag fibers. This degree of silverization is threefold greater than that attainable for the unmodified M13-Ag fibers. Conferred bactericidal functionality is determined via live-dead staining and a modified disk-diffusion (Kirby-Bauer) measure of zone of inhibition (Zol) against Staphylococcus epidermidis and Escherichia coli bacterial strains. Live-dead staining and Zol distance measurements indicate increased bactericidal activity in the genetically engineered virus fibers attached to silver. Coating of Kevlar fibers with E3 viruses exhibits antibacterial effects, as well, with relatively smaller ZoIs attributable to the lower degree of silver loading attainable in these coatings. Such antimicrobial functionality is amenable to rapid incorporation within fiber-based textiles to reduce risks of infection, biofilm formation, or odor-based detection, with the potential to exploit the additional electronic and thermal conductivity of fully silverized fibers and coatings.

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Section: Introductionsupporting

confidence: 54%

Genetically Engineered Phage Fibers and Coatings for Antibacterial Applications

Mao

Belcher

Vliet

2010

Adv Funct Materials

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“…Ampicillin (6) [33] (Figure 4) was bound on the surface of this matrix by chemical bonding in organic medium. The amount of Ampicillin chemically bound to the matrix was spectroscopically characterized and the in vitro release rate of Ampicillin in weak basic medium was established along with the determination of its antimicrobial activity.…”

Section: Immobilization Of Antibiotics or Othersmentioning

confidence: 99%

“…Also Ikeda et al [35] studied the interaction of the polyionenes synthesized in the above study [33] with phospholipid bilayer membranes by means of DSC with special reference to their antimicrobial activities. A strong interaction was observed between the polyionenes and acidic phospholipids, whereas zwitterionic phosphonic bilayers were not affected significantly by the polycations.…”

Section: Water-soluble Polymers With Quaternary Ammonium Saltsmentioning

confidence: 99%

Antibacterial and Bacterium Adsorbing Macromolecules

Tashiro¹

2001

Macromol. Mater. Eng.

318

105

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“…Therefore, surface modification by incorporating with antibacterial agents to inhibit the adhesion of bacteria and microbes on the Ti-based implants should an effective route to enhance the success ratios of clinical treatments for Ti-based metallic implants. 18−21 Tibased implants have been endowed with self-antibacterial properties via various strategies which include titanate nanowires (TNWs) loaded with antibiotics, 22,23 inorganic bactericide doped TNWs, 24−26 bioactive antibacterial polymerization, 27,28 nonantibiotic organic bactericide loaded TNWs, 29,30 and adhesion-resistant TNWs. 31 However, the fast release of the antibacterial agent would cause safety concerns, and the emergence of resistance strains is another issue when excessive antibiotics are administered.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Silver Doped Titanate Nanowires on Ti Implants

et al. 2016

ACS Appl. Mater. Interfaces

105

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A nanostructured film composed of one-dimensional titanate nanowires (TNWs) was employed as a carrier of Ag nanoparticles and chitosan (CS) to improve the surface antibacterial activity and biocompatibility of titanium implants. A TNWs film was produced on a Ti substrate by an alkali hydrothermal reaction and subsequently doped by Ag nanoparticles through an ultraviolet light chemical reduction. The CS nanofilm was deposited on the Ag nanoparticles through a spin-assisted layer by layer assembly method. The results disclosed that Ag nanoparticles were successfully carried by TNWs and homogeneously distributed on the entire surface. Moreover, a CS nanofilm was also successfully deposited on the Ag nanoparticles. Antibacterial tests showed that the samples modified with a higher initial concentration of AgNO3 solution exhibited better antibacterial activity, and that a CS nanofilm could further improve the antibacterial activity of the TNWs. Cell viability and ALP tests revealed that the release of Ag(+) was detrimental for the growth, proliferation, and differentiation of MC3T3, and that CS could lower the negative effects of Ag gradually as the incubation time increased.

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Bioactive polymers: Synthesis, release study and antimicrobial properties of polymer bound Ampicillin

Cited by 32 publications

References 10 publications

Genetically Engineered Phage Fibers and Coatings for Antibacterial Applications

Genetically Engineered Phage Fibers and Coatings for Antibacterial Applications

Antibacterial and Bacterium Adsorbing Macromolecules

Antibacterial Activity of Silver Doped Titanate Nanowires on Ti Implants

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