Bioactive Lipoxygenase Metabolites Stimulation of NADPH Oxidases and Reactive Oxygen Species

Cho, Kyung Jin; Seo, Ji Min; Kim, Jae Hong

doi:10.1007/s10059-011-1021-7

Cited by 154 publications

(103 citation statements)

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“…[16][17][18] ROS generation has also been found to be important for LPS signaling in macrophages, and LPS-induced ROS generation in these cells is attenuated by DPI, an inhibitor of flavoenzymes, including Nox. [19][20][21] We therefore investigated whether BLT2-dependent, Nox-mediated ROS generation might contribute to LPS-induced IL-6 synthesis in mouse peritoneal macrophages.…”

Section: Nox1-ros Signaling Functions Downstream Of Blt2 In Lpsinducementioning

confidence: 99%

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

2015

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Endotoxic responses to bacterial lipopolysaccharide (LPS) are triggered by Toll-like receptor 4 (TLR4) and involve the production of inflammatory mediators, including interleukin-6 (IL-6), by macrophages. The detailed mechanism of IL-6 production by macrophages in response to LPS has remained unclear, however. We now show that LPS induces IL-6 synthesis in mouse peritoneal macrophages via the leukotriene B 4 receptor BLT2. Our results suggest that TLR4-MyD88 signaling functions upstream of BLT2 and that the generation of reactive oxygen species (ROS) by NADPH oxidase 1 (Nox1) and consequent activation of the transcription factor nuclear factor (NF)-κB function downstream of BLT2 in this response. These results suggest that a TLR4-MyD88-BLT2-Nox1-ROS-NF-κB pathway contributes to the synthesis of IL-6 in LPS-stimulated mouse macrophages.

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Section: Nox1-ros Signaling Functions Downstream Of Blt2 In Lpsinducementioning

confidence: 99%

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

2015

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“…The actions of cyclooxygenases and lipoxygenases during conversion of arachidonic acid, which can be released into the cytoplasm from membrane phospholipids due to the activity of phospholipase A2, also contribute to intracellular formation of ROS (68). Another cytoplasmic source for O 2 -is XOR, a molybdoflavoprotein, which exists in the two interconvertible forms xanthine oxidase (XO) and xanthine dehydrogenase (XDH).…”

Section: Fig 2 Ros Generation In Relation Tomentioning

confidence: 99%

Nutritional Countermeasures Targeting Reactive Oxygen Species in Cancer: From Mechanisms to Biomarkers and Clinical Evidence

Samoylenko

Hossain²,

Mennerich³

et al. 2013

Antioxidants & Redox Signaling

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Reactive oxygen species (ROS) exert various biological effects and contribute to signaling events during physiological and pathological processes. Enhanced levels of ROS are highly associated with different tumors, a Western lifestyle, and a nutritional regime. The supplementation of food with traditional antioxidants was shown to be protective against cancer in a number of studies both in vitro and in vivo. However, recent largescale human trials in well-nourished populations did not confirm the beneficial role of antioxidants in cancer, whereas there is a well-established connection between longevity of several human populations and increased amount of antioxidants in their diets. Although our knowledge about ROS generators, ROS scavengers, and ROS signaling has improved, the knowledge about the direct link between nutrition, ROS levels, and cancer is limited. These limitations are partly due to lack of standardized reliable ROS measurement methods, easily usable biomarkers, knowledge of ROS action in cellular compartments, and individual genetic predispositions. The current review summarizes ROS formation due to nutrition with respect to macronutrients and antioxidant micronutrients in the context of cancer and discusses signaling mechanisms, used biomarkers, and its limitations along with large-scale human trials.

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“…In recent years, a growing body of evidence suggests that activity of NADPH oxidase (NOX) is stimulated by LT B 4 via the LT B 4 receptor 2 (BLT2). BLT2-stimulated NOX1 upregulation is a downstream signaling route that mediates the synthesis of Th2 cytokines (IL-4 and IL-13) and the generation of NOX1-derived ROS [17,18]. …”

Section: Introductionmentioning

confidence: 99%

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

Dilek

Özkaya²,

Koçyiğit³

et al. 2015

Int Arch Allergy Immunol

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Background: There is ample knowledge reported in the literature about the role of oxidative stress in asthma pathogenesis. It is also known that the interaction of reactive oxygen species with DNA may result in DNA strand breaks. The aim of this study was to investigate if montelukast monotherapy affects oxidative stress and DNA damage parameters in a population of pediatric asthma patients. Methods: Group I consisted of 31 newly diagnosed asthmatic patients not taking any medication, and group II consisted of 32 patients who had been treated with montelukast for at least 6 months. Forty healthy control subjects were also enrolled in the study. Plasma total oxidant status (TOS) and total antioxidant status (TAS) were measured to assess oxidative stress. DNA damage was assessed by means of alkaline comet assay. Results: The patients in both group I and group II had statistically significant higher plasma TOS (13.1 ± 4 and 11.1 ± 4.1 μmol H₂O₂ equivalent/liter, respectively) and low TAS levels (1.4 ± 0.5 and 1.5 ± 0.5 mmol Trolox equivalent/liter, respectively) compared with the control group (TOS: 6.3 ± 3.5 μmol H₂O₂ equivalent/liter and TAS: 2.7 ± 0.6 mmol Trolox equivalent/liter; p < 0.05). DNA damage was 18.2 ± 1.0 arbitrary units (a.u.) in group I, 16.7 ± 8.2 a.u. in group II and 13.7 ± 3.4 a.u. in the control group. There were statistically significant differences only between group I and the control group (p < 0.05). Conclusions: According to the findings, montelukast therapy makes only minimal but not statistically significant improvement in all TOS, TAS and DNA damage parameters.

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Bioactive Lipoxygenase Metabolites Stimulation of NADPH Oxidases and Reactive Oxygen Species

Cited by 154 publications

References 50 publications

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

MyD88–BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage

Nutritional Countermeasures Targeting Reactive Oxygen Species in Cancer: From Mechanisms to Biomarkers and Clinical Evidence

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

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