Bioactive ingredients obtained from Cortex Fraxini impair interactions between FAS and GPI

Wu, Songtao; Li, Tong; Liu, Bo; Ai, Zhongzhu; Hong, Zongchao; Peng, You; H, Wu; Yang, Yanfang

doi:10.1016/j.freeradbiomed.2019.11.022

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…In the present study, ARU, CL, APN, and CR had higher CF, NDF, and ADF contents than the other Chinese herbs, suggesting that these herbs can be used as dietary fiber supplements to maintain the intestinal health of animals. However, previous studies were mostly focused on the anti-inflammatory or hypoglycemic effects of CL, APN, and CR [21][22][23], lacking the research progress on dietary fiber as feed supplements in livestock production. Thus, it is necessary to conduct in-depth research on Chinese herbs as a fiber source for animals' growth and development in the future.…”

Section: Discussionmentioning

confidence: 99%

“…The CO 2 was continuously flushed into the culture solution for 30 min at 37 • C (pH 6.9-7.0), and then added 60 mL of culture solution into the bottle under a stream of CO 2 at 37 • C. Bottles were immediately placed into the constant temperature shaker (ZWY-200 D; Zhicheng Instruments Co., Ltd., Shanghai, China) for 48 h at 37 • C and 55 r/min. The gas production was recorded at 0.5, 1, 2, 4, 6,8,10,12,14,16,18,20,22,24,30,36, and 48 h.…”

Section: In Vitro Batch Incubationmentioning

confidence: 99%

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Nutritional Values and In Vitro Fermentation Characteristics of 29 Different Chinese Herbs

Xiao

Azad

et al. 2022

Agriculture

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The present study evaluated the nutritional values and in vitro fermentation characteristics of 29 different Chinese herbs in order to develop effective feed additives for livestock nutrition. The Chinese herbs were analyzed for their chemical composition. The in vitro fermentation characteristics were evaluated using the gas production technique with swine cecal chyme inoculum. The results showed that the Allium tuberosum Rottl (Semen allii tuberosi, SAT) had the highest gross energy (GE), crude protein (CP), ether extract (EE), and total amino acid (TAA) contents, whereas Anemones raddeanae Rhizoma (Agstache rugosus, AR) had the highest crude fiber, neutral detergent fiber, and acid detergent fiber contents compared with the other Chinese herbs. The Rhus chinensis Mill (Chinese gall, CG), Scrophularia ningpoensis Hemsl (Radix scrophulariae, RS), Punica granatum L (Punica granatum L, PGL), Atractylodes macrocephala Koidz (White atractylodes rhizome, WAR), Sanguisorbae radix (Garden burnet, GB), Anemarrhena asphodeloides Bunge (Rhizoma anemarrhenaw, RA), and Rhei radix Et Rhizoma (Rheum officinale, RO) had a higher non-fiber carbohydrate content than the other Chinese herbs. The in vitro fermentation kinetic analysis showed that the RS, WAR, and RA had a higher gas production, a theoretical gas production, and a fractional rate of gas production than the other groups. Furthermore, the RA, WAR, RS, and Crataegi fructus (Crataegus pinnatifida Bun, CPB) had a higher total short-chain fatty acids (SCFAs) concentration, and CPB had the highest acetate concentration, whereas the WAR and RS had higher propionate and butyrate concentrations than the other groups. Moreover, the Isatis indigotica Fort (Folium isatidis, FI) and SAT had higher ammonia-N concentration than the other groups. In summary, among the 29 different Chinese herbs, the SAT had the highest nutritional value, which is reflected in the highest contents of GE, CP, EE, and TAA, whereas the RA, WAR, and RS showed better intestinal fermentation profiles, which is reflected in the higher fermentation degree and suitable SCFAs fermentation pattern. These findings provide a theoretical basis for using Chinese herbs as feed additives or microbial carbon sources in the intestines of animals.

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Section: Discussionmentioning

confidence: 99%

Section: In Vitro Batch Incubationmentioning

confidence: 99%

Nutritional Values and In Vitro Fermentation Characteristics of 29 Different Chinese Herbs

Xiao

Azad

et al. 2022

Agriculture

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“…3.6.1, ; accessed on 2 December 2021) [ 36 ]. Further, the topological characteristics of PPI networks were also analyzed using Cytoscape’s analytical tool [ 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

Computer-Aided Screening and Revealing Action Mechanism of Food-Derived Tripeptides Intervention in Acute Colitis

Zhang

Tang

et al. 2022

IJMS

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Food-derived tripeptides can relieve colitis symptoms; however, their alleviation mode has not been systematically evaluated as an alternative nutritional compound. This study aimed to reveal the potential mechanism of 8000 food-derived tripeptides against acute colitis using a computer-aided screening strategy. Forty-one potential hub targets related to colitis with a Fit score > 4.0 were screened to construct the protein-protein and protein-tripeptide network based on the PharmMapper database and STRING software (Ver. 11.5). In addition, 30 significant KEGG signaling pathways with p-values < 0.001 that the 41 hub targets mainly participated in were identified using DAVID software (Ver. 6.8), including inflammatory, immunomodulatory, and cell proliferation and differentiation-related signaling pathways, particularly in the Ras- and PI3K-Akt signaling pathways. Furthermore, molecular docking was performed using the Autodock against majorly targeted proteins (AKT1, EGFR, and MMP9) with the selected 52 tripeptides. The interaction model between tripeptides and targets was mainly hydrogen-bonding and hydrophobic interactions, and most of the binding energy of the tripeptide target was less than −7.13 kcal/mol. This work can provide valuable insight for exploring food-derived tripeptide mechanisms and therapeutic indications.

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“…Cortex fraxini medicine contains a variety of chemical components [1,2]. Modern pharmacological studies have shown that C. fraxini peel has antibacterial and antiviral, anti-inflammatory, analgesic and sedative, anti-tumor, antitussive, expectorant and asthma, promoting uric acid excretion and diuresis [3][4][5]. Studies have reported that C. fraxini contains coumarin, iridoids, lignin, phenols, sterols and triterpenoids, tannins, alkaloids, and other chemical components.…”

Section: Introductionmentioning

confidence: 99%

Determination of fraxetin, fraxin and dimethylfraxetin in rat plasma by UPLC-MS/MS

Jia,

Wang,

Wang

et al. 2024

AChrom

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ObjectiveThe levels of fraxetin, fraxin, and dimethylfraxetin in rat plasma to be measured using an ultra-performance liquid chromatography tandem mass-spectrometry (UPLC–MS/MS) technique and applied to their pharmacokinetics and bioavailability.MethodsThe protein precipitation technique was applied to the plasma preparation using acetonitrile and methanol (9:1, v/v). At a flow rate of 0.4 mL min−1, the elution time was 6 min. The mobile phase consisted of acetonitrile-water with 0.1% formic acid, and the chromatographic column was UPLC HSS T3 (50 mm × 2.1 mm, 1.8 μm). Quantitative analysis was conducted using multiple reaction monitoring (MRM) mode and detection was performed using electrospray ionization (ESI) positive ion mode. In each group, six rats were treated with fraxetin, fraxin, and dimethylfraxetin either orally (5 mg kg−1) or intravenously (1 mg kg−1).ResultsThe calibration curves showed good linearity in the range of 2–4,000 ng mL−1, where r was greater than 0.99. The bioavailability of dimethylfraxetin, fraxin, and fraxetin was determinated to be 19.7, 1.4, and 6.0%.ConclusionThe established UPLC-MS/MS method for determining the levels of these three compounds in rat plasma was successfully applied to the pharmacokinetics of dimethylfraxetin, fraxin, and fraxetin, and the bioavailability was calculated.

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Bioactive ingredients obtained from Cortex Fraxini impair interactions between FAS and GPI

Cited by 13 publications

References 25 publications

Nutritional Values and In Vitro Fermentation Characteristics of 29 Different Chinese Herbs

Nutritional Values and In Vitro Fermentation Characteristics of 29 Different Chinese Herbs

Computer-Aided Screening and Revealing Action Mechanism of Food-Derived Tripeptides Intervention in Acute Colitis

Determination of fraxetin, fraxin and dimethylfraxetin in rat plasma by UPLC-MS/MS

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