Bioactive glass‐based surfaces induce differential gene expression profiling of osteoblasts

Abstract

The ability of Biosilicate® with two crystalline phases (BioS-2P) to drive osteoblast differentiation encourages the investigation of the cellular mechanisms involved in this process. Then, the aim of our study was to analyze the large-scale gene expression of osteoblasts grown on BioS-2P compared with Bioglass 45S5 (45S5). Osteoblasts differentiated from rat bone marrow mesenchymal stem cells were cultured under osteogenic conditions on BioS-2P, 45S5 and polystyrene (control). After 10 days, the expression of 23,794 genes was analyzed using mRNA Sequencing and the data were validated by real-time PCR. The BioS-2P exhibited 5 genes upregulated and 3 downregulated compared with 45S5. Compared with control, BioS-2P upregulated 15 and downregulated 11 genes, while 45S5 upregulated 25 and downregulated 21 genes. Eight genes were commonly upregulated and 4 downregulated by both bioactive glasses. In conclusion, our results demonstrated that bioactive glasses affect the gene expression profiling of osteoblasts. Most of the regulated genes by both BioS-2P and 45S5 are associated with the process of mineralization highlighting their osteostimulation property that is, at least in part, derived from the ability to modulate the intracellular machinery to promote osteoblast genotype expression. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 419-423, 2017.

Citrate-based materials fuel human stem cells by metabonegenic regulation

“…The metabonegenic mechanism may be worthy of future study to explore whether nutrient-or energysensing pathways (e.g., the mTOR and the AMPK pathways) are involved in citrate metabonegenic regulation, especially since citrate uptake increased mTOR-dependent protein synthesis and diminished the hypothalamic AMPK activity following exogenous supplementation (40). Moreover, the involvement and upregulation of SLC13a5 rather than SLC16a1 as the lactate membrane transporter during active bone formation (10,11,41) highlights the advantages of citrate-based materials for orthopedic applications compared with traditional PLA-based materials.…”

Effect of cell source and osteoblast differentiation on gene expression profiles of mesenchymal stem cells derived from bone marrow or adipose tissue

“…43,44 The osteoblastic differentiation has been characterized by the gene expression of bone markers, alkaline phosphatase activity, and deposition of mineralized matrix. [43][44][45] Regarding the origin, the clustering of samples showed that MSCs from both sources present similar gene expression profiles, but differences in the expression of genes directly related to the processes of osteoblast differentiation and osteogenesis, such as Ibsp, Alpl, and Dlx5, suggest that BM-MSCs are more committed to osteoblast differentiation, which positively affects the osteogenic potential of these cells. For instance, the overexpression of Dlx5 inhibits adipocyte differentiation while inducing osteoblast differentiation of MSCs.…”

Bioactive glass containing 90% SiO 2 in hard tissue engineering: An in vitro and in vivo characterization study

“…In fact, the growth of an apatite‐like layer may provide an ideal environment for osteoblast colonization, proliferation, and differentiation (Jell & Stevens, ; Zhao et al, ). Moreover, BG extracts deeply impact the gene expression of osteoblasts, and this effect may be, at least in part, associated with their osteostimulation ability (de Godoy et al, ; Ferraz et al, ; Xynos et al, ). The up‐regulation of genes previously recognized as participating in the mineralization process (e.g., Bglap, Dmp1, Rcor2, and Phospho1) indicates a more advanced stage of osteoblast differentiation when these cells are cultured in contact with BGs (Ferraz et al, ).…”

“…Therefore, chemical signals delivered from BGs, mainly ionic products, have been widely reported to promote matrix synthesis of fibroblasts and angiogenic differentiation of endothelial cells (Gorustovich, Roether, & Boccaccini, 2010;Shih, Lu, Hsieh, Chen, & Chen, 2014). The calcium phosphate layer spontaneously produced after BG contact with body fluids can form a chemical bond with bone and dissolve over time, provoking bone cell activity through the up-regulation of several genes in osteoblasts (Ferraz et al, 2017;Hoppe et al, 2011;Xynos, Edgar, Buttery, Hench, & Polak, 2001). All these characteristics have made them attractive to many applications in tissue engineering, particularly in orthopaedic and dentistry fields (Habraken, Habibovic, Epple, & Bohner, 2016).…”