Abstract:The potential of cantabrian anchovy (Engraulis encrarischolus) viscera as a source of bioactive compounds is of interest for both, pharmaceutical and food industries. Cantabrian anchovy guts and heads were freeze-dried, extracted with methanol and subjected to fractionation by solvent partitioning using hexane, ethyl acetate, and butanol. Fractions were tested for antimutagenic, antioxidant, antifungal, and antibacterial activity using the Ames test; DPPH, ABTS, and FRAP assays; the radial grown inhibition ass… Show more
“…According to Burgos-Hernández et al [23] and Ramos-Hernández et al [10], the composition of the primary level of the extract, prevents HBVC from fully expressing their biological effects. Hence, a separation into fractions by column chromatography is required.…”
Section: Fractionation Of Extract By Column Chromatography and Qualit...mentioning
The triterpenes have low solubility, as a strategy to facility its transport into the gastrointestinal tract, the techno-functional properties of the mixture of high degree of polymerization Agave fructans (HDPAF) and whey protein (WP) as encapsulating material for stabilized Coccoloba uvifera L extract fraction were evaluated. The purification of the extract was carried out by column chromatography, obtaining the fraction with the highest bioactivity. The microstructure evaluated by SEM showed capsules with spherical shapes using electrospraying. HPLC-MS analysis indicated the presence of β-sitosterol and lupeol in the encapsulates. The main interactions of biopolymers and compounds were by FTIR. The Tg of the capsules (> 149 °C) and the thermal stability (> 202 °C) of the compounds were by thermal analysis (DSC and TGA, respectively). The loading and encapsulation efficiency were 100 and 95%, respectively. Finally, the encapsulates presented antioxidant capacities for ABTS, DPPH, and FIC (between 90 and 71%). The HDPAF-WP mixture has techno-functional properties that allow the stabilization of HBVC by electrospraying.
“…According to Burgos-Hernández et al [23] and Ramos-Hernández et al [10], the composition of the primary level of the extract, prevents HBVC from fully expressing their biological effects. Hence, a separation into fractions by column chromatography is required.…”
Section: Fractionation Of Extract By Column Chromatography and Qualit...mentioning
The triterpenes have low solubility, as a strategy to facility its transport into the gastrointestinal tract, the techno-functional properties of the mixture of high degree of polymerization Agave fructans (HDPAF) and whey protein (WP) as encapsulating material for stabilized Coccoloba uvifera L extract fraction were evaluated. The purification of the extract was carried out by column chromatography, obtaining the fraction with the highest bioactivity. The microstructure evaluated by SEM showed capsules with spherical shapes using electrospraying. HPLC-MS analysis indicated the presence of β-sitosterol and lupeol in the encapsulates. The main interactions of biopolymers and compounds were by FTIR. The Tg of the capsules (> 149 °C) and the thermal stability (> 202 °C) of the compounds were by thermal analysis (DSC and TGA, respectively). The loading and encapsulation efficiency were 100 and 95%, respectively. Finally, the encapsulates presented antioxidant capacities for ABTS, DPPH, and FIC (between 90 and 71%). The HDPAF-WP mixture has techno-functional properties that allow the stabilization of HBVC by electrospraying.
“…Suárez-Jiménez et al (2015) reported that collagen hydrolysates from jumbo squid by-products exhibited an effective inhibition of the induced mutation with AFB 1 of S. typhimurium TA98 and TA100 strains. Additionally, Burgos-Hernández et al (2016) investigated the antimutagenic potential of several fractions obtained from anchovy viscera against AFB 1 using S. typhimurium TA98 and TA100 tester strains. Dn the other hand, several studies reported the antimutagenic activity of some compounds obtained from seafood products such as white shrimp (López-Saiz et al, 2016), octopus (Cruz-Ramírez et al, 2015), and some other marine resources such as several species of seaweed (Dsuna-Ruiz et al, 2016).…”
“…Lately, there is a particular interest in researching the antioxidant activity of fish viscera, e.g., Abdelhedi et al (2017) had obtained low molecular weight (MW) peptide (<1 kDa) by the ultrafiltration from smooth hound viscera protein hydrolysates and the peptide exhibited good antioxidant capacity. Burgos-Hernandez et al (2016) investigated bioactive fractions from cantabrian anchovy ( Engraulis encrarischolus ) viscera and the fractions showed the antioxidant activity through various assays. In addition, there were many other studies on fish viscera protein hydrolysates ( Villamil, Vaquiro & Solanilla, 2017 ).…”
Mackerel (Pneumatophorus japonicus) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 °C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various in vitro assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.