Encapsulation with HDPAF-WP of the hexane fraction of sea grape (Coccoloba uvifera L.) leaf extract by electrospraying

Abstract

The triterpenes have low solubility, as a strategy to facility its transport into the gastrointestinal tract, the techno-functional properties of the mixture of high degree of polymerization Agave fructans (HDPAF) and whey protein (WP) as encapsulating material for stabilized Coccoloba uvifera L extract fraction were evaluated. The purification of the extract was carried out by column chromatography, obtaining the fraction with the highest bioactivity. The microstructure evaluated by SEM showed capsules with spherical shapes using electrospraying. HPLC-MS analysis indicated the presence of β-sitosterol and lupeol in the encapsulates. The main interactions of biopolymers and compounds were by FTIR. The Tg of the capsules (> 149 °C) and the thermal stability (> 202 °C) of the compounds were by thermal analysis (DSC and TGA, respectively). The loading and encapsulation efficiency were 100 and 95%, respectively. Finally, the encapsulates presented antioxidant capacities for ABTS, DPPH, and FIC (between 90 and 71%). The HDPAF-WP mixture has techno-functional properties that allow the stabilization of HBVC by electrospraying.