Bioactive fish collagen/polycaprolactone composite nanofibrous scaffolds fabricated by electrospinning for 3D cell culture

Choi, Da Jeong; Choi, Seung Mi; Kang, Hae Yeong; Min, Hye-Jin; Lee, Rira; Ikram, Muhammad; Subhan, Fazli; Jin, Song Wan; Jeong, Young Hun; Kwak, Jong‐Young; Yoon, Sik

doi:10.1016/j.jbiotec.2015.01.017

Cited by 65 publications

(29 citation statements)

References 35 publications

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“…20 As rotational speeds increased, BJPNF diameter decreased significantly for each composition. Furthermore, we also observed a significant increase in the fiber diameters of samples with a higher ratio of PCL compared to experimental groups with a higher ratio of gelatin, which is consistent with previous studies done using collagen, 21 as well as previous reports of CJSspun fibers. 5…”

Section: Characterization Of Bjpnf Alignment and Diametersupporting

confidence: 92%

Engineering anisotropic biphasic Janus‐type polymer nanofiber scaffold networks via centrifugal jet spinning

et al. 2016

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Biphasic materials, comprised of an ordered arrangement of two different material phases within a material, have the potential for a wide variety of applications including filtration, protective clothing and tissue engineering. This study reports for the first time, a process for engineering biphasic Janus-type polymeric nanofiber (BJPNF) networks via the centrifugal jet spinning technique. BJPNF alignment and fiber diameter was dependent on fabrication rotational speed as well as solution composition. The biphasic character of these BJPNFs, which was controlled via the rotational speed of fabrication, was confirmed at the individual nanofiber scale using energy dispersive X-ray spectroscopy, and at the bulk, macro-scale using attenuated total reflectance-Fourier transform infrared spectroscopy. Biphasic character was also demonstrated at the functional level via differing affinities on either side of the BJPNF for cell attachment. Our work thus presents a method for fabricating BJPNF scaffold networks where there might be a need for different properties on either side of a material. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2455-2464, 2017.

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Section: Characterization Of Bjpnf Alignment and Diametersupporting

confidence: 92%

Engineering anisotropic biphasic Janus‐type polymer nanofiber scaffold networks via centrifugal jet spinning

et al. 2016

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“…Consistent with our findings, Liu and Sun [22] also reported that tilapia-derived FSCP with molecular weights ranging from 0.7 to 1.3 kDa promoted the growth of rat bone marrow mesenchymal stem cells. In addition, results from our previous study suggested that tilapia FSCP contained in the FC/PCL nanofibrous scaffolds contribute to enhanced proliferation of mouse thymic epithelial cells [43]. Furthermore, Liu et al [44] showed that bovine collagen peptide compounds promoted the proliferation and differentiation of MC3T3-E1 preosteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Fish Scale Collagen Peptides Protect against CoCl₂/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells

Subhan

Kang

Lim

et al. 2017

Oxidative Medicine and Cellular Longevity

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Skin diseases associated with inflammation or oxidative stress represent the most common problem in dermatology. The present study demonstrates that fish scale collagen peptides (FSCP) protect against CoCl2-induced cytotoxicity and TNF-α-induced inflammatory responses in human HaCaT keratinocyte cells. Our study is the first to report that FSCP increase cell viability and ameliorate oxidative injury in HaCaT cells through mechanisms mediated by the downregulation of key proinflammatory cytokines, namely, TNF-α, IL-1β, IL-8, and iNOS. FSCP also prevent cell apoptosis by repressing Bax expression, caspase-3 activity, and cytochrome c release and by upregulating Bcl-2 protein levels in CoCl2- or TNF-α-stimulated HaCaT cells. In addition, the inhibitory effects of FSCP on cytotoxicity and the induction of proinflammatory cytokine expression were found to be associated with suppression of the ROS, MAPK (p38/MAPK, ERK, and JNK), and NF-κB signaling pathways. Taken together, our data suggest that FSCP are useful as immunomodulatory agents in inflammatory or immune-mediated skin diseases. Furthermore, our results provide new insights into the potential therapeutic use of FSCP in the prevention and treatment of various oxidative- or inflammatory stress-related inflammation and injuries.

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“…Unlike them, collagen, the main constituent of the ECM of most tissue types, is capable of providing cell attachment sites and thus activate integrin binding signaling pathways. 56 Not surprisingly, collagen has also been studied for its ability to enrich the CSC population. Chen et al observed that a 3D collagen scaffold promoted overexpression of pro-angiogenic growth factors, enhanced the tumorigenicity of breast cancer cells in vivo, upregulated EMT markers, and enriched the CSC population.…”

Section: Biomaterials For Csc Growthmentioning

confidence: 99%

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

Ordikhani

Kim

Zustiak

2015

JOM

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The theory of cancer stem cells (CSCs) and their role in cancer metastasis, tumorigenicity and resistance to therapy is slowly shifting the emphasis on the search for cancer cure: more evidence is surfacing that a successful therapy should be geared against this rare cancer cell population. Unfortunately, CSCs are difficult to culture in vitro which severely limits the progress of CSC research. This review gives a brief overview of CSCs and their microenvironment, with particular focus on studies that used in vitro biomaterial-based models and biomaterial/CSC interfaces for the enrichment of CSCs. Biomaterial properties relevant to CSC behaviors are also addressed. While the discussed research field is still in its infancy, it appears that in vitro cancer models that include a biomaterial can support CSC enrichment and this has proved indispensable to the study of their biology as well as the development of novel cancer therapies.

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Bioactive fish collagen/polycaprolactone composite nanofibrous scaffolds fabricated by electrospinning for 3D cell culture

Cited by 65 publications

References 35 publications

Engineering anisotropic biphasic Janus‐type polymer nanofiber scaffold networks via centrifugal jet spinning

Engineering anisotropic biphasic Janus‐type polymer nanofiber scaffold networks via centrifugal jet spinning

Fish Scale Collagen Peptides Protect against CoCl₂/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

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Bioactive fish collagen/polycaprolactone composite nanofibrous scaffolds fabricated by electrospinning for 3D cell culture

Cited by 65 publications

References 35 publications

Engineering anisotropic biphasic Janus‐type polymer nanofiber scaffold networks via centrifugal jet spinning

Engineering anisotropic biphasic Janus‐type polymer nanofiber scaffold networks via centrifugal jet spinning

Fish Scale Collagen Peptides Protect against CoCl2/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

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Fish Scale Collagen Peptides Protect against CoCl₂/TNF-α-Induced Cytotoxicity and Inflammation via Inhibition of ROS, MAPK, and NF-κB Pathways in HaCaT Cells