Bioactive Compounds of Horse Gram (Macrotyloma uniflorum Lam. [Verdc.])

Ingle, Krishnananda Pralhad; Al–Khayri, Jameel M.; Chakraborty, Pritam; Narkhede, Gopal Wasudeo; Suprasanna, Penna

doi:10.1007/978-3-030-44578-2_36-1

Cited by 6 publications

(3 citation statements)

References 94 publications

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“…S14 ). The lysine significance in horse gram is well reported earlier 39 . The order of other EAA are phenylalanine, threonine, valine, leucine, and histidine.…”

Section: Stability Analysissupporting

confidence: 70%

Assessing the yield and nutrient potential of horse gram mutants (Macrotyloma uniflorum Lam. Verdc.) an underutilized legume through a multi-environment-based experiment

Jafarullakhan,

Vijayakumar,

Singh

et al. 2024

Sci Rep

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The agronomic stability and nutritional importance of 30 (Test genotypes: 29 + Check: 1 = 30) promising horse gram mutants were evaluated in this multi-environment-based experiment (MEE). Attempts were made to (i) identify stable mutants for agronomic traits through AMMI and GGE biplot models, (ii) quantify nutritional traits, (iii) understand the linkage between yield and nutritional traits, and (iv) estimate physical (PP) and cooking properties (CP) of selected genotypes to fix their food-chain usability. The ANOVA of the pooled data exhibited significant differences among environments (E), genotypes (G), and GxE interaction. The combined AMMI and GGE results helped to identify a few good-yielding and stable genotypes (GYSM) (G1, G25, G3, and G27). The yield advantages of these GYSMs over the parent PAIYUR 2 are 42.99%, 34.63%, 28.68%, and 30.59% respectively. The nutrient profiling of mutants revealed (i) a significant coefficient of variation for macronutrients (fat: 29.98%; fibre: 20.72%, and protein: 5.01%), (ii) a good range of variation for micronutrients, and (iii) helped to identify macro (MaNSM) and micro nutrient-specific mutants (MiNSM). The relationship analysis between yield and nutrient traits ascertained that yield had (i) positivity with protein (r2 = 0.69) and negativity for micronutrients except for Mn (r2 = 0.63), Cu (r2 = 0.46), and B (r2 = 0.01) in GYSM, (ii) positivity with protein and fibre in MaNSM, and (iii) negativity with micronutrients in MiNSM. Of the GYSM, G1 and G25 offer scope for commercial exploitation, and their PP and CP analyses revealed that G1 can be used for pastry and baked product preparation while G25 for weaning foods. Cooking time exhibited positivity with seed size parameters and negativity with water absorption capacity (r2 = − 0.53). An LC–MS–MS-based amino acid (AA) fractionation study showed the effect of induced mutagenesis on the contents of amino acids and also revealed the significance of horse gram for its lysine and methionine contents.

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“…S14 ). The lysine significance in horse gram is well reported earlier 39 . The order of other EAA are phenylalanine, threonine, valine, leucine, and histidine.…”

Section: Stability Analysissupporting

confidence: 70%

Assessing the yield and nutrient potential of horse gram mutants (Macrotyloma uniflorum Lam. Verdc.) an underutilized legume through a multi-environment-based experiment

Jafarullakhan,

Vijayakumar,

Singh

et al. 2024

Sci Rep

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“…Horse gram proteins exhibit free radical scavenging activities which make them suitable as a food supplement and natural antioxidant [55]. Bioactive peptides of the legume's protein have also been reported to possess antimicrobial, antioxidant, anticarcinogenic, and hypocholesterolemic activities [56].…”

Section: Nutrient Content and Health Benefits Of Underutilized Legumesmentioning

confidence: 99%

Forgotten Gems: Exploring the Untapped Benefits of Underutilized Legumes in Agriculture, Nutrition, and Environmental Sustainability

Odeku,

Ogunniyi,

Ogbole

et al. 2024

Plants

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In an era dominated by conventional agricultural practices, underutilized legumes termed “Forgotten Gems” represent a reservoir of untapped benefits with the unique opportunity to diversify agricultural landscapes and enhance global food systems. Underutilized crops are resistant to abiotic environmental conditions such as drought and adapt better to harsh soil and climatic conditions. Underutilized legumes are high in protein and secondary metabolites, highlighting their role in providing critical nutrients and correcting nutritional inadequacies. Their ability to increase dietary variety and food security emerges as a critical component of their importance. Compared to mainstream crops, underutilized legumes have been shown to reduce the environmental impact of climate change. Their capacity for nitrogen fixation and positive impact on soil health make them sustainable contributors to biodiversity conservation and environmental balance. This paper identifies challenges and proposes strategic solutions, showcasing the transformative impact of underutilized legumes on agriculture, nutrition, and sustainability. These “Forgotten Gems” should be recognized, integrated into mainstream agricultural practices, and celebrated for their potential to revolutionize global food production while promoting environmental sustainability.

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“…In many parts of India, Macrotyloma uniflorum grains are commonly consumed as a green vegetable and are believed to be high in protein and have medicinal benefits ( Prasad and Singh, 2015 ). Horse Gram seeds contain bioactive compounds such as phenolic acids and isoflavonones, which play a key role in enhancing antioxidant enzymes in cancer cells thus imparting anticancer properties ( Ingle, et al, 2020 ). Horsegram, is used for asthma, bronchitis, leukoderma, urine discharges, kidney stones, and heart disease, according to traditional medical sources ( Marimuthu and Krishnamoorthi, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

2-Dimensional in vitro culture assessment of ovarian cancer cell line using cost effective silver nanoparticles from Macrotyloma uniflorum seed extracts

Lavudi

Harika

Kokkanti

et al. 2022

Front. Bioeng. Biotechnol.

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Our research focused on generating AgNPs using Macrotyloma uniflorum (MU) seed extracts and studied their efficacy in combating tumor growth using the 2-Dimensional method for ovarian cancer cell line-PA-1. Characterization studies including a UV-visible spectrophotometer confirmed the surface plasmon resonance peak of 436 nm. Particle size determination data validated the nanoparticle diameter of 91.8 nm. Synthesized AgNPs possess a negative charge of -28.0 mV, which was confirmed through the zeta potential study. Structural characterization studies including XRD determined the crystal phase of AgNPs at four distant peaks at 2θ (38.17, 44.36, 64.52, and 77.46) and were assigned to 111, 200, 220, and 311 planes of the FCC. FTIR studies have confirmed the presence of O-H, N-H, C=O, ethers, C-Br, and C-I groups in AgNPs respectively. DPPH study has confirmed the presence of free radicles and we observed that at 500 μg/ml concentration, 76.08% of free radicles were formed which shows their efficiency. MTT assay shows the efficacy of MU-AgNPs in reducing the cell viability. At lower concentrations of MU-AgNP, 66% viability was observed and 9% of viability was observed at higher dose. ROS production (21%) was observed using MU-AgNPs with respect to 0.45% in controls, which affirms the capacity to induce DNA damage via apoptosis. Standard drug camptothecin generated 26% of ROS production which confirms higher potential of AgNPs in inducing DNA damage in tumor cells without causing lethality to the healthy cells. Further, the Fluorescence-activated cell sorting (FACS) study using a standard Caspase-3 marker confirms the generation of apoptotic bodies using two different concentrations of MU-AgNPs. At 40 μg, 64% of apoptotic cell death was observed, whereas, using 20 μg, 23% of apoptosis was recorded via fluorescent intensity. Propidium iodide-based Cell cycle study has shown a significant decrease in G0/G1 phase compared to control (88.8%), which further confirmed the apoptotic induction. Matrix metalloproteinases (MMP) studies using JC-1 dye, showed a significant increase in green fluorescence owing to lowered membrane potential, thus ensuring the breakdown of mitochondrial potential compared to untreated and standard drugs. With the obtained results, we are concluding that MU-AgNPs has a tremendous capacity to suppress the ovarian cancer cell proliferation in vitro by inducing DNA damage and apoptosis.

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Bioactive Compounds of Horse Gram (Macrotyloma uniflorum Lam. [Verdc.])

Cited by 6 publications

References 94 publications

Assessing the yield and nutrient potential of horse gram mutants (Macrotyloma uniflorum Lam. Verdc.) an underutilized legume through a multi-environment-based experiment

Assessing the yield and nutrient potential of horse gram mutants (Macrotyloma uniflorum Lam. Verdc.) an underutilized legume through a multi-environment-based experiment

Forgotten Gems: Exploring the Untapped Benefits of Underutilized Legumes in Agriculture, Nutrition, and Environmental Sustainability

2-Dimensional in vitro culture assessment of ovarian cancer cell line using cost effective silver nanoparticles from Macrotyloma uniflorum seed extracts

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