No abstract

No abstract

No abstract

Our research focused on generating AgNPs using Macrotyloma uniflorum (MU) seed extracts and studied their efficacy in combating tumor growth using the 2-Dimensional method for ovarian cancer cell line-PA-1. Characterization studies including a UV-visible spectrophotometer confirmed the surface plasmon resonance peak of 436 nm. Particle size determination data validated the nanoparticle diameter of 91.8 nm. Synthesized AgNPs possess a negative charge of -28.0 mV, which was confirmed through the zeta potential study. Structural characterization studies including XRD determined the crystal phase of AgNPs at four distant peaks at 2θ (38.17, 44.36, 64.52, and 77.46) and were assigned to 111, 200, 220, and 311 planes of the FCC. FTIR studies have confirmed the presence of O-H, N-H, C=O, ethers, C-Br, and C-I groups in AgNPs respectively. DPPH study has confirmed the presence of free radicles and we observed that at 500 μg/ml concentration, 76.08% of free radicles were formed which shows their efficiency. MTT assay shows the efficacy of MU-AgNPs in reducing the cell viability. At lower concentrations of MU-AgNP, 66% viability was observed and 9% of viability was observed at higher dose. ROS production (21%) was observed using MU-AgNPs with respect to 0.45% in controls, which affirms the capacity to induce DNA damage via apoptosis. Standard drug camptothecin generated 26% of ROS production which confirms higher potential of AgNPs in inducing DNA damage in tumor cells without causing lethality to the healthy cells. Further, the Fluorescence-activated cell sorting (FACS) study using a standard Caspase-3 marker confirms the generation of apoptotic bodies using two different concentrations of MU-AgNPs. At 40 μg, 64% of apoptotic cell death was observed, whereas, using 20 μg, 23% of apoptosis was recorded via fluorescent intensity. Propidium iodide-based Cell cycle study has shown a significant decrease in G0/G1 phase compared to control (88.8%), which further confirmed the apoptotic induction. Matrix metalloproteinases (MMP) studies using JC-1 dye, showed a significant increase in green fluorescence owing to lowered membrane potential, thus ensuring the breakdown of mitochondrial potential compared to untreated and standard drugs. With the obtained results, we are concluding that MU-AgNPs has a tremendous capacity to suppress the ovarian cancer cell proliferation in vitro by inducing DNA damage and apoptosis.

Several plants have been studied to find their efficacy and anti-cancer activity in various cancers by synthesizing organic metal nanoparticles. However, usage of Semecarpus anacardium (SA) and production of green synthesized nanoparticles have not been exposed. In our study, we have focused on synthesizing silver nanoparticles using the nut extracts from SA. Characterization studies including UV-Visible spectrophotometry have confirmed the silver nanoparticle formation at 412 nm using 0.1 mM and 427 nm using 0.2 mM AgNPs. Particle size was recorded at 1.4 nm confirming their effectivity and zeta potential studies confirmed the respective charge of -38.6 mV of the particle. Anti-microbial activity was shown against gram negative bacteria. MTT assay studies confirmed the anti-cancer activity against ovarian cancer cell line, PA-1. These results depict the excellent cytotoxic effect on the PA-1 ovarian cancer cell line, with an IC50 value of 250 µg/ml. Flow cytometry studies confirmed that SA methanolic nut extracts inhibited cell cycle at G0/G1 phase and induced apoptosis. Taken together, we are confirming that SA methanolic extracts have anti-cancer properties against ovarian cancer cell line, PA-1.

Background: Several reports have shown the beneficial advantages of Musa paradisiaca in wound healing activities and other ailments. Previously, our in vitro studies validated the anti-cancer activities of Musa flower extracts and confirmed the potential. This thread has led to our current investigation to explore the anticancer potential in vivo. Purpose: This study focused on the efficacy of banana florets on DMBA induced breast cancer in female wistar rats. Methods: Induction of tumour using Dimethylbenzanthracene (DMBA) in female wistar rats. Hormonal, antioxidant and anticarcinogenic studies were performed in vivo. Results: In our current study, we investigated that tumour induction has an effect in disrupting the estrous cycle in mice which may result by hormonal fluctuation levels. Antioxidant and hormonal analysis in vivo revealed the imbalance in estrogen and progesterone levels in untreated group compared to the treated ones. Floral crude extract treatment in vivo has shrunken the tumour volume in flower extract treatment group as well as in standard drug compared to the control. Histopathological staining confirms the disruption of epithelial tissues in tumour induced groups compared to the treated ones. Moreover, Musa floral treatment has shown to revert the damaged tissue morphology in the treated groups compared to the saline treated one. In-vitro studies in MCF-7 and MDA-MB-231 breast cancer cell lines has shown the potent anti-tumorigenic activity using Musa floral extracts. Conclusion: Taken together, our findings confirmed that banana flower extracts showcase anti-carcinogenic activity against breast cancer both in vitro and in vivo. Tumour induction in mice has an effect in showing the disruption of estrous cycles.