Bioactive Compounds in Lipid Fractions of Pumpkin (<i>Cucurbita sp</i>) Seeds for Use in Food

Veronezi, Carolina Médici; Jorge, Neuza

doi:10.1111/j.1750-3841.2012.02736.x

Cited by 65 publications

(28 citation statements)

References 27 publications

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“…Plants belonging to the Cucurbitaceae family may possess pharmacological properties (Talukdar & Hossain, 2014;Vijayakumar, Eswaran, Ojha, Rao Ch & Rawat, 2011), including antidiabetic role (Chandrasekar, Mukherjee & Mukherjee, 1989;Huseini, Darvishzadeh, Heshmat, Jafariazar, Raza & Larijani, 2009;Rashidi, Mirhashemi, Taghizadeh & Sarkhail, 2013 ), anti-ulcer role, analgesic, nephro-protection (Jain & Singhai, 2010 ), and anticancer effects (Vijayakumar et al, 2011 ).Pumpkin (Cucurbita pepo L) was extensively investigated for uses in herbal therapy (Abdel-Rahman, 2006) and as a source of bioactive food compounds (Veronezi & Jorge, 2012). Less wellinvestigated cucurbitaceae are now receiving attention related to both food and medicinal applications (Milind & Kulwant, 2011;Talukdar & Hossain, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components

et al. 2016

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Cucurbitaceae are one of most widely used plant species for human food but lesser known members have not been examined for bioactive components. The purpose of this study was to evaluate the antioxidant and genoprotective activities from three cucurbitaceae seeds extracts and to identify phenolic components by LC-ESIMS/MS analysis. From the results, the yield of seeds extract was 20-41% (w/w) and samples had 16-40% total phenols as gallic acid equivalents (GAE). Compared with methanol solvent, using acidified methanol led to increased extraction yield by 1.4 to 10-fold, higher phenolic content (149.5 ± 1.2 to 396.4 ± 1.9 mg GAE/g), higher DPPH radical quenching and enhanced genoprotective activity using the pBR322 plasmid assay. LC-ESI-MS/MS analysis led to identification of 14-17 components, based on authentic standards and comparison with literature reports, as mainly phenolic acids and esters, flavonol glycosides. This may be the first mass spectrometric profiling of polyphenol components from cucurbitaceae seeds.

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Section: Introductionmentioning

confidence: 99%

Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components

et al. 2016

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“…It was also found that γ-tocopherol is the isomer that stood out in the lipid fractions and in the seeds, mainly in Menina Brasileira (Veronezi and Jorge, 2012).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory activity of two varieties of pumpkin seed oil in an adjuvant arthritis model in rats

et al. 2017

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SUMMARY:The aim of the present research was to evaluate the anti-inflammatory activity of pumpkin seed oils (PSOs) of an Egyptian and European variety, in a rat model of adjuvant arthritis. Edema thickness, plasma tumor necrosis factor-α (TNF-α) and erythrocyte sedimentation rate (ESR) were determined as inflammatory biomarkers while malondialdehyde (MDA) and total antioxidant capacity (TAC) were assessed as indicative of oxidative stress. Chromosomal aberration, sperm shape abnormalities, and DNA fragmentations are cytogenetic parameters which aid in tracing inflammatory and oxidative activity. Phenolic contents and β-carotene were determined in PSOs. The results showed elevated ESR, plasma TNF-α, plasma MDA, liver cellular DNA fragmentation, bone marrow chromosomal aberration, sperm shape abnormalities with a reduction in plasma TAC and body weight gain in an adjuvant arthritis control compared to a healthy control. Administration of low and high doses of either Egyptian or European PSO improved all the aforementioned parameters with variable degrees.KEYWORDS: Adjuvant arthritis; Anti-inflammatory; β-Carotene; Phenolic contents; Pumpkin seed oils RESUMEN: Actividad antiinflamatoria de dos variedades de aceite de semillas de calabaza en un modelo de artritis adyuvante en ratas. El objetivo de la presente investigación fue evaluar la actividad antiinflamatoria de aceites de calabaza (PSOs) de variedades egipcia y europea, en un modelo de rata con artritis adyuvante. El espesor del edema, el factor de necrosis tumoral (TNF-α) y la velocidad de sedimentación eritrocitaria (ESR) se determinaron como biomarcadores inflamatorios, mientras que el malondialdehído (MDA) y la capacidad antioxidante total (TAC) fueron evaluados como indicativos de estrés oxidativo. La aberración cromosómica, las anomalías de la forma del esperma y las fragmentaciones del ADN son parámetros citogenéticos que ayudan a localizar la actividad inflamatoria y oxidativa. Se determinaron contenidos fenólicos y β-caroteno en PSOs. Los resultados mostraron elevado ESR, TNF-α plasmático, MDA plasmática, fragmentación del ADN del hígado, aberración cromosómica de la médula ósea, anomalías de la forma espermática con una reducción del TAC plasmático y un aumento del peso corporal en el control de la artritis adyuvante en comparación con el control sano. La administración de dosis bajas y altas de PSO egipcia o europea mejoró todos los parámetros mencionados en grados variables.

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“…Several species of great economic interest are included in this group, with the most popular being cucumber (Cucumis sativus L.), watermelon (Citrullus vulgaris S.), melon (Cucumis melo L.) and summer and winter pumpkin (Cucurbita spp.). Among the pumpkin species belonging to the genus Cucurbita, the most cultivated in the world are Cucurbita pepo L., C. moschata L. and C. maxima L. (GONZAGA et al, 1999;LOY, 2004;PARIS and BROWN, 2005;VERONEZI and JORGE, 2012).…”

Section: Introductionmentioning

confidence: 99%

First report and molecular characterization of an isolate of papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba

Rodríguez-Martínez¹,

Figueira²,

Duarte³

et al. 2015

Biosci. J.

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ABSTRACT:In this work, a virus isolate collected from pumpkin plants (Cucurbita pepo L.), showing severe symptoms of mosaic and leaf deformation, grown in Cuba, was analyzed using indicator plants, electron microscopy, and phylogenetic analysis. Plants of pumpkin, cv. Caserta, inoculated with this virus isolate showed mosaic, leaf distortion and blistering symptoms, whereas papaya plants were immune and did not show any symptoms. A transmission electron microscopic examination of leaf dip preparations made from infected pumpkin leaves revealed the presence of elongated and flexuous particles, approximately 780-800 x 12 nm in size. Genomic fragments containing the coat protein (CP) and HC-Pro genes, amplified by specific primers for Papaya ringspot virus, W strain (PRSV-W), showed amino acid identities of both genes higher than 94% when compared to other PRSV-W isolates from America. In the phylogenetic tree, this virus isolate has grouped with other virus isolates from America, Australia, and India and was more distant from the Asian isolates. Taken together, the analyses allow the conclusion that this virus isolate is a W strain of PRSV, detected for the first time in Cuba.

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Bioactive Compounds in Lipid Fractions of Pumpkin (Cucurbita sp) Seeds for Use in Food

Cited by 65 publications

References 27 publications

Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components

Antioxidant and genoprotective activity of selected cucurbitaceae seed extracts and LC–ESIMS/MS identification of phenolic components

Anti-inflammatory activity of two varieties of pumpkin seed oil in an adjuvant arthritis model in rats

First report and molecular characterization of an isolate of papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba

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