Bioactive components, antioxidant and DNA damage inhibitory activities of honeys from arid regions

Habib, Hosam M.; Meqbali, Fatima T. Al; Kamal, Hina; Souka, Usama; Ibrahim, Wissam H.

doi:10.1016/j.foodchem.2013.12.044

Cited by 82 publications

(72 citation statements)

References 24 publications

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“…Honey varieties were selected for in vitro analysis based on the results reported by Habib et al [21]. Four honey varieties were selected from the arid region, two monofloral (H1 and H3) and two heterofloral (H2 and H4), to compare with two non-arid region varieties H5 (monofloral) and H6 (heterofloral).…”

Section: Methodsmentioning

confidence: 99%

“…The climate where honey is made also significantly influences the polyphenol content and profile. Notably, it was demonstrated that some arid region honey possess a greater amount of polyphenols compared to famous non-arid region honey [21, 22]. Knowing this, it is reasonable to expect from arid region honey greater health benefits.…”

Section: Introductionmentioning

confidence: 99%

“…Knowing this, it is reasonable to expect from arid region honey greater health benefits. Habib et al [21] reported that one or more types of honey from arid regions presented a higher antioxidant potential compared to non-arid region honey varieties. Habib et al [21] compared non arid region honey varieties such as Black Forest and Manuka to arid region honey by using biochemical assays.…”

Section: Introductionmentioning

confidence: 99%

“…Habib et al [21] reported that one or more types of honey from arid regions presented a higher antioxidant potential compared to non-arid region honey varieties. Habib et al [21] compared non arid region honey varieties such as Black Forest and Manuka to arid region honey by using biochemical assays. Non-arid region varieties of honey such as Manuka [23], Gelam [16] and Tualang [24] have been extensively studied in regards to their antioxidant, anti-inflammatory and anti-tumor properties.…”

Section: Introductionmentioning

confidence: 99%

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Bioactivity of arid region honey: an in vitro study

Hilary

Habib

Souka

et al. 2017

BMC Complement Altern Med

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BackgroundAntioxidant and anti-inflammatory properties of honey have been largely recognized by various studies. Almost all of the potential benefits are associated with polyphenol content. Honey varieties from the arid region are reported to be rich in polyphenols, but data related to its bioactivity in vitro is greatly lacking. This study aimed at establishing the antioxidant and anti-inflammatory properties of arid region honey. Four honey varieties from arid region (H1, H2, H3, and H4) and two popular non-arid region honey (H5 and H6) were tested in vitro in this study.MethodsThe erythrocyte membrane protection effect of honey varieties were measured by hemolysis assay after exposing erythrocytes to a peroxide generator. The subsequent production of MDA (malondialdehyde) content in erythrocytes was measured. Immunomodulatory effect of the honey varieties was tested in prostate cancer cells PC-3 and PBMC (peripheral blood mononuclear cells) by measuring the IL-6 (interleukin 6) and NO (nitric oxide) levels in cell culture supernatant after incubation with the honey varieties. PC-3 cell viability was assessed after incubation with honey varieties for 24 h.ResultsArid region honey exhibited superior erythrocyte membrane protection effect with H4 measuring 1.3 ± 0.042mMTE/g and H2 measuring 1.122 ± 0.018mMTE/g. MDA levels were significantly reduced by honey samples, especially H4 (20.819 ± 0.63 nmol/mg protein). We observed a significant decrease in cell population in PC-3 after 24 h in culture on treatment with honey. A moderate increase in NO levels was observed in both cultures after 24 h at the same time levels of IL-6 were remarkably reduced by honey varieties.ConclusionThe results demonstrate the antioxidant effect of arid region honey due to its erythrocyte membrane protection effect and subsequent lowering of oxidative damage as evident from lower levels of lipid peroxidation byproduct MDA. Arid region honey varieties were as good as non-arid region types at decreasing cell viability of prostate cancer cells. The moderate increase in NO levels in PC-3 and PBMCs were not significant enough to elicit any pro-inflammatory response. However, IL-6 secretion was remarkably reduced by all honey varieties in a comparable level indicating the potential anti-inflammatory property of arid region honey.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioactivity of arid region honey: an in vitro study

Hilary

Habib

Souka

et al. 2017

BMC Complement Altern Med

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“…A wide variety of compounds isolated from honey and propolis come from lora, region and climate diferences, where the nectar or sap was collected [12,48,49]. The phenolic compounds extracted, isolated and characterized can be classiied into two major groups: phenolic acids and lavonoids.…”

Section: Evaluation Of the Phenolic Contentmentioning

confidence: 99%

Honey as a Functional Food

Luchese¹,

Prudêncio²,

Guerra³

2017

Honey Analysis

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The most well-known functional properties of honey are its antioxidant and antimicrobial activities. The bioactive components of honey are afected by the lora from which it is produced and by geographical variations. Phenolic compounds promote, among other activities, high antioxidant action, being capable of minimizing intracellular oxidative damage associated with cellular aging, apoptosis and neurodegenerative diseases. A living cell system would provide a beter platform for determining antioxidant activity, since the bioactive honey compounds can act modulating antioxidant defense gene expression. Indeed, phenolic compounds, amino acids and reducing sugars are among the substances responsible for honey antioxidant activity. Most of phenolic compounds also exert antimicrobial activity against a number of pathogens and spoilage microorganisms. The antimicrobial activity of honey is also due to the action of enzymes. In addition, honey was found to contain lactic acid bacteria (LAB), which itself produce a myriad of active compounds that remain in variable amounts in mature honey. In addition, these antioxidant compounds might play a key role as prebiotic, protecting and stimulating growth of probiotic bacteria. Oligosaccharides present in honey are well-known prebiotic substances stimulating growth, activity and protecting probiotic bacteria during passage through the gastrointestinal tract and during storage of the products. This chapter describes the main bioactive components of honey, especially with respect to the phenolic compounds and their antioxidant activity and assay methods.

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Chemical Profile, Antioxidant and Enzyme Inhibition Activities of Natural Saudi Sidr and Talh Honeys

Qahtani

Yagi

Yılmaz

et al. 2022

Chemistry & Biodiversity

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Honey is used since ancient time as a food and to cure many diseases. The present study investigated the chemical constituents, antioxidant and enzyme inhibition activities of natural Saudi Sidr (SH) and Talh (TH) honeys. Beside entire honey samples, ethyl acetate, ethanol and water extracts were prepared. The total polyphenolic content of SH, TH and their extracts was in the range of 2.86–7.21 and 3.80–17.33 mg gallic acid equivalents/g, respectively and the total flavonoids content was in the range of 0.05–1.17 and 0.18–2.38 mg rutin equivalents/g, respectively. Out of the 53 standards analyzed by HPLC, 27 compounds were detected with highest number of compounds identified in the ethyl acetate extract of TH (45 %, 24/53) and SH (26 %, 14/53), respectively. Quinic acid was dominant compound identified in all honey samples with the highest content determined in TH ethanol extract (4454 μg/g). The majority of tested samples possessed considerable anti‐radicals and reducing ions capacity with the ethyl acetate extract from TH exerted significantly (p<0.05) the highest activity. All honey samples did not show chelating iron metal property. Honey samples revealed variable enzyme inhibition activity with TH (entire and/or ethyl acetate extract) showed significantly (p<0.05) the highest acetylcholinesterase, butyrylcholinesterase, tyrosinase and α‐amylase inhibition activity. In conclusion, ethyl acetate is the best solvent for extraction of bioactive molecules from the two honey types. Moreover, the dark‐colored TH contained the highest number of molecules and consequently exerted the best antioxidant and enzyme inhibition activities in most assays.

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Bioactive components, antioxidant and DNA damage inhibitory activities of honeys from arid regions

Cited by 82 publications

References 24 publications

Bioactivity of arid region honey: an in vitro study

Bioactivity of arid region honey: an in vitro study

Honey as a Functional Food

Chemical Profile, Antioxidant and Enzyme Inhibition Activities of Natural Saudi Sidr and Talh Honeys

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