Bioactive components and properties of ethanolic extract and its fractions from Gynura procumbens leaves

Kaewseejan, Niwat; Siriamornpun, Sirithon

doi:10.1016/j.indcrop.2015.05.019

Cited by 51 publications

(43 citation statements)

References 37 publications

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“…Inhibitory potential of all light-treated callus cultures against advanced glycation end products (AGEs) formation was determined according to modified protocol of Kaewseejan and Siriamornpun [107]. Sample extracts from each treatment were mixed with the solution of BSA (Sigma Aldrich) 20 mg/mL in the presence of phosphate (0.1 M) buffer at pH 7.4 comprising 0.02% NaN 3 (sodium azide w/v).…”

Section: Inhibition Of Ages Formationmentioning

confidence: 99%

Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of Solanum xanthocarpum Callus Cultures

et al. 2020

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Solanum xanthocarpum is considered an important traditional medicinal herb because of its unique antioxidant, and anti-diabetic, anti-aging, and anti-inflammatory potential. Because of the over exploitation linked to its medicinal properties as well as destruction of its natural habitat, S. xanthocarpum is now becoming endangered and its supply is limited. Plant in vitro culture and elicitation are attractive alternative strategies to produce biomass and stimulate biosynthesis of medicinally important phytochemicals. Here, we investigated the potential influence of seven different monochromatic light treatments on biomass and secondary metabolites accumulation in callus culture of S. xanthocarpum as well as associated biological activities of the corresponding extracts. Among different light treatments, highest biomass accumulation was observed in white light-treated callus culture. Optimum accumulation of total flavonoid contents (TFC) and total phenolic contents (TPC) were observed in callus culture kept under continuous white and blue light respectively than control. Quantification of phytochemicals through HPLC revealed that optimum production of caffeic acid (0.57 ± 0.06 mg/g DW), methyl-caffeate (17.19 mg/g ± 1.79 DW), scopoletin (2.28 ± 0.13 mg/g DW), and esculetin (0.68 ± 0.07 mg/g DW) was observed under blue light callus cultures. Compared to the classic photoperiod condition, caffeic acid, methyl-caffeate, scopoletin, and esculetin were accumulated 1.7, 2.5, 1.1, and 1.09-folds higher, respectively. Moreover, high in vitro cell free antioxidant, anti-diabetic, anti-aging, and anti-inflammatory activities were closely associated with the production of these secondary metabolites. These results clearly showed the interest to apply multispectral light as elicitor of in vitro callus cultures S. xanthocarpum to promote the production of important phytochemicals, and allow us to propose this system as an alternative for the collection of this endangered species from the wild.

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Section: Inhibition Of Ages Formationmentioning

confidence: 99%

Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of Solanum xanthocarpum Callus Cultures

et al. 2020

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“…Anti-AGEs formation activity. Kaewseejan and Siriamornpun [33] method was used to determine inhibitory potential of AGEs (advanced glycation end products) formation. Extract preparation was carried out at a concentration of 50μg/ml in DMSO mixed with 0.5 M glucose and 20 mg/ml BSA solution, both were prepared in phosphate buffer and 1 ml of 0.1 M phosphate buffer containing 0.02% (w/v) sodium azide at pH 7.4.…”

Section: Antidiabetic Activity Of Callus Extractsmentioning

confidence: 99%

Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus culture of Eclipta alba L.

et al. 2020

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Eclipta alba L., also known as false daisy, is well known and commercially attractive plant with excellent hepatotoxic and antidiabetic activities. Light is considered a key modulator in plant morphogenesis and survival by regulating important physiological cascades. Current study was carried out to investigate growth and developmental aspects of E. alba under differential effect of multispectral lights. In vitro derived callus culture of E. alba was exposed to multispectral monochromatic lights under controlled aseptic conditions. Maximum dry weight was recorded in culture grown under red light (11.2 g/L) whereas negative effect was observed under exposure of yellow light on callus growth (4.87 g/L). Furthermore, red light significantly enhanced phenolics and flavonoids content (TPC: 57.8 mg/g, TFC: 11.1 mg/g) in callus cultures compared to rest of lights. HPLC analysis further confirmed highest accumulation of four major compounds i.e. coumarin (1.26 mg/g), eclalbatin (5.00 mg/g), wedelolactone (32.54 mg/g) and demethylwedelolactone (23.67 mg/g) and two minor compounds (β-amyrin: 0.38 mg/g, luteolin: 0.39 mg/g) in red light treated culture whereas stigmasterol was found optimum (0.22 mg/g) under blue light. In vitro based biological activities including antioxidant, antidiabetic and lipase inhibitory assays showed optimum values in cultures exposed to red light, suggesting crucial role of these phytochemicals in the enhancement of the therapeutic potential of E. alba. These results clearly revealed that the use of multispectral lights in in vitro cultures could be an effective strategy for enhanced production of phytochemicals.

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“…2 Oxidative processes in the human body produce free radicals as by products, primarily reactive oxygen species (ROS) and it is associated with cancer occurrence. 3 Elevated levels of ROS and down regulation of ROS scavengers and antioxidant enzymes are associated with various human diseases, including cancers. 4 Hence, new effective alternative treatments are required to improve outcomes for patients with malignant gliomas.…”

Section: Introductionmentioning

confidence: 99%

Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

Rohin¹,

Jumli²,

Ridzwan³

et al. 2018

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Objective: Gynura procumbens (Lour.) Merr is a well-known traditional herb and is widely used for traditional medicine by human. Previous clinical studies have shown the benefit effect of this leaves with inflammation, high blood pressure and others. This study aims to investigate the effect of methanol, ethanol, and ethyl acetate extracts of Gynura procumbens on U-87 cell line, human Glioblastoma multiforme cell line. Methods: In the present study, effect of methanol, ethanol and ethyl acetate extracts on U-87 cell line was determined by MTT-based anti-proliferative assay and any significant changes to the cells towards apoptotic changes was observed under a light microscope. Results: The ethanol and methanol extracts of Gynura procumbens was found to have high anti-proliferative effect on U-87 cell line with IC 50 less than 20µg/ml. By comparing IC 50 value, ethanol and methanol extracts of Gynura procumbens had high potential for anti-proliferative effect on U-87 cell line. Therefore, ethanol and methanol extracts can be a potential anti-proliferative effect on U-87 cell line. Conclusion: To the best of our knowledge, this is the first study of exploring the effect of Gynura procumbens extracts using three different polarity (methanol, ethanol, ethyl acetate) effect on U-87 cell line, Human Glioblastoma multiforme cells.

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Bioactive components and properties of ethanolic extract and its fractions from Gynura procumbens leaves

Cited by 51 publications

References 37 publications

Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of Solanum xanthocarpum Callus Cultures

Interactive Effects of Wide-Spectrum Monochromatic Lights on Phytochemical Production, Antioxidant and Biological Activities of Solanum xanthocarpum Callus Cultures

Lights triggered differential accumulation of antioxidant and antidiabetic secondary metabolites in callus culture of Eclipta alba L.

Effect of Gynura procumbens Extracts on Anti-Proliferative Activity and its Associated Morphological Changes of Human Glioblastoma multiforme Cell Line (U-87)

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