Bioactive antiinflammatory antibacterial hemostatic citrate-based dressing with macrophage polarization regulation for accelerating wound healing and hair follicle neogenesis

Abstract

The efficient cutaneous wound healing accompanied with the enhanced skin appendage regeneration is still a challenge. The bacterial infection and excessive/prolonged inflammation inhibit wound healing process and result in the scar formation. Herein, we reported an anti-inflammatory polycitrate-polyethyleneimine-Ibuprofen (PCEI) and multifunctional PCEI-based F127-ε-polypeptide-alginic (FEA) dressing (FEA-PCEI) for accelerating wound healing and hair follicle neogenesis. PCEI showed the excellent anti-inflammation function through stimulating macrophage towards anti-inflammatory M2 subtype polarization. The FEA-PCEI dressing showed the temperature-response gelation, injectability, robust antibacterial activity, light-damage-resistant, homeostasis ability, and good cytocompatibility. The optimized dosage of FEA-PCEI dressing could significantly accelerate wound healing with anti-infection ability, reduce the scar formation, and promote the hair follicle neogenesis. This study provided a wound-repairing strategy through regulating the phenotype of immune cells by the designing bioactive multifunctional biomaterials.

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

“…7 b indicate the hematoxylin-eosin (H&E) staining of the experimental groups (PLLA membrane and 20% PNA membrane) and control group. At 7 d, abundant inflammatory cells were infiltrated into the upper layers of dermis in the wound site in the control group, possibly because severe inflammation occurred at the wound site due to the lack of external protection [ 9 , 10 ]. Compared with the control group, mice treated with PLLA membrane displayed a significant decrease in inflammatory cells at the wound site.…”

“…Wound healing is a complicated process generally consisting of four successive and overlapped steps: (1) hemostasis phase; (2) inflammatory phase; (3) proliferative phase; and (4) maturation and remodeling phase [ 9 , 10 ]. Among them, inflammatory phase is a kind of significant procedure, where immune cells secrete pro-inflammatory cytokines to induce inflammatory cells to produce a large amount of reactive oxygen species (ROS), which are the important metabolites of oxygen, including peroxides, superoxide, the hydroxyl radical and singlet oxygen [ 11 ].…”

Injectable muscle-adhesive antioxidant conductive photothermal bioactive nanomatrix for efficiently promoting full-thickness skeletal muscle regeneration

“…Recently, the biomedical polymers based on citric acid have been developed and attracted much attention, due to their good biocompatibility, biomimetic viscoelasticity and low cost [ 24 , 25 ]. Our group also reported a series of citric acid-based hybrid polymers and demonstrated their promising biomedical applications in bioimaging, drug delivery/obesity therapy and skin regeneration [ [26] , [27] , [28] , [29] , [30] ]. Recent studies exhibited that citric acid-based polymers could efficiently improve the angiogenesis which was the vital step during the tissue formation [ 31 ].…”

“…PPy@PDA was used to achieve photothermal effect, antioxidant ability and electronic conductivity. CMC and PCE which could stimulate cell proliferation with low cytotoxicity in our previous report [ 29 ], were served as the crosslinked network. F127-Phe-CHO was served as the temperature-sensitive component, which formed the gel network by Schiff-base reaction between its aldehyde group and quinone group on PPy@PDA and amino group of CMC and PCE.…”

3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing

“…interleukins, monocytes and neutrophils) [ 45 ]. Excessive/prolonged inflammation may inhibit wound healing and an anti-inflammatory dressing can accelerate wound healing [ 46 ]. In addition, re-epithelialization is an essential event during the wound healing process.…”