Bioactive antibacterial bilayer PCL/gelatin nanofibrous scaffold promotes full-thickness wound healing

Jafari, Arman; Amirsadeghi, Armin; Hassanajili, Shadi; Azarpira, Negar

doi:10.1016/j.ijpharm.2020.119413

Cited by 102 publications

(63 citation statements)

References 94 publications

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“…However, previous experiments have shown that PCL NF alone does not affect cell growth. 66 , 67 In addition, the relatively short experimental period provided insufficient evidence to support the long-term solubility of the VEGF-A loaded NF membrane. The degradation of PCL scaffolds is very slow, which remains a challenge.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of VEGF-A Nanofiber Membrane and FAAH Inhibitor URB597 Against Oxygen–Glucose Deprivation-Induced Ischemic Neuronal Injury

Wang¹,

Jin²,

Zhao

et al. 2021

IJN

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Introduction Brain ischemia is a common neurological disorder worldwide that activates a cascade of pathophysiological events involving decreases in oxygen and glucose levels. Despite substantial efforts to explore its pathogenesis, the management of ischemic neuronal injury remains an enormous challenge. Accumulating evidence suggests that VEGF modified nanofiber (NF) materials and the fatty-acid amide hydrolase (FAAH) inhibitor URB597 exert an influence on alleviating ischemic brain damage. We aimed to further investigate their effects on primary hippocampal neurons, as well as the underlying mechanisms following oxygen–glucose deprivation (OGD). Methods Different layers of VEGF-A loaded polycaprolactone (PCL) nanofibrous membranes were first synthesized by using layer-by-layer (LBL) self-assembly of electrospinning methods. The physicochemical and biological properties of VEGF-A NF membranes, and their morphology, hydrophilicity, and controlled-release of VEGF-A were then estimated. Furthermore, the effects of VEGF-A NF and URB597 on OGD-induced mitochondrial oxidative stress, inflammatory responses, neuronal apoptosis, and endocannabinoid signaling components were assessed. Results The VEGF-A NF membrane and URB597 can not only promote hippocampal neuron adhesion and viability following OGD but also exhibited antioxidant/anti-inflammatory and mitochondrial membrane potential protection. The VEGF-A NF membrane and URB597 also inhibited OGD-induced cellular apoptosis through activating CB1R signaling. These results indicate that VEGF-A could be controlled-released by LBL self-assembled NF membranes. Discussion The VEGF-A NF membrane and URB597 displayed positive synergistic neuroprotective effects through the inhibition of mitochondrial oxidative stress and activation of CB1R/PI3K/AKT/BDNF signaling, suggesting that a VEGF-A loaded NF membrane and the FAAH inhibitor URB597 could be of therapeutic value in ischemic cerebrovascular diseases.

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Section: Discussionmentioning

confidence: 99%

Neuroprotective Effects of VEGF-A Nanofiber Membrane and FAAH Inhibitor URB597 Against Oxygen–Glucose Deprivation-Induced Ischemic Neuronal Injury

Wang¹,

Jin²,

Zhao

et al. 2021

IJN

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“…Many studies have shown the excellent mechanical properties of synthetic polymers such as polycaprolactone (PCL), polyurethane (PU) and poly (lactic-co-glycolic acid) (PLGA) due to their thermal and chemical stability [78,79]. These polymers could also be blended with natural polymers such as chitosan, collage and gelatin, which have excellent biocompatibility in order to improve their mechanical properties [80][81][82].…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis

et al. 2021

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Skin is the body’s first barrier against external pathogens that maintains the homeostasis of the body. Any serious damage to the skin could have an impact on human health and quality of life. Tissue engineering aims to improve the quality of damaged tissue regeneration. One of the most effective treatments for skin tissue regeneration is to improve angiogenesis during the healing period. Over the last decade, there has been an impressive growth of new potential applications for nanobiomaterials in tissue engineering. Various approaches have been developed to improve the rate and quality of the healing process using angiogenic nanomaterials. In this review, we focused on molecular mechanisms and key factors in angiogenesis, the role of nanobiomaterials in angiogenesis, and scaffold-based tissue engineering approaches for accelerated wound healing based on improved angiogenesis.

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“…The in vitro drug release assessments demonstrated that hybrid nanofibers have a sustained release manner for Zn nanoparticles and amoxicillin up to 144 h. The antibacterial analysis of the dual drug-loaded hybrid nanofibers in vitro employing the disk diffusion method exhibited the larger inhibition zone size between 13.42 ± 0.65 and 17.32 ± 0.42 mm against S. aureus, indicating good antibacterial efficacy. The wound healing experiments using circular full-thickness wounds on Sprague-Dawley rats revealed that the wound contraction percentage for the control was 64.77 ± 3.35, and 95.07 ± 1.51% on days 6 and 10 respectively, while dual drug-loaded hybrid nanofibers displayed a wound contraction percentage of 69.44 ± 3.65, and 95.60 ± 2.99% at the same time, respectively [ 105 ]. Basara et al produced electrospun gelatin–PCL wound dressing nanofibers for controlled drug release of ketoprofen, an anti-inflammatory drug.…”

Section: Fabrication Of Biopolymer-based Hybrid Nanofibersmentioning

confidence: 99%

Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications

Alven

Aderibigbe

2021

Polymers

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The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.

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Bioactive antibacterial bilayer PCL/gelatin nanofibrous scaffold promotes full-thickness wound healing

Cited by 102 publications

References 94 publications

Neuroprotective Effects of VEGF-A Nanofiber Membrane and FAAH Inhibitor URB597 Against Oxygen–Glucose Deprivation-Induced Ischemic Neuronal Injury

Neuroprotective Effects of VEGF-A Nanofiber Membrane and FAAH Inhibitor URB597 Against Oxygen–Glucose Deprivation-Induced Ischemic Neuronal Injury

Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis

Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications

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