Bioaccumulation‐based silver nanoparticle toxicity in Daphnia magna and maternal impacts

Abstract

In the present study, we tested whether bioaccumulation in specific tissues of Daphnia magna could explain silver nanoparticle (AgNP) toxicity. Daphnids were exposed to different concentrations of well-suspended AgNPs and AgNO . The accumulations of Ag in the whole body, gut, and nongut tissues, as well as the mortality of daphnids, were recorded over a period of 7 d. Regression analysis showed a higher degree of positive correlation between the concentration of Ag in the nongut tissues than gut tissues and the mortality of daphnids. The results strongly suggested that the toxicity of AgNPs could be better explained in terms of bioaccumulation of AgNPs in the nongut tissues. We further tested the maternal transfer of AgNPs in daphnids into the next generation using radioactive tracers, which were able to detect as low as 1.0 to 3.2% of total accumulated Ag transferred to the neonates. The AgNPs significantly affected the reproduction process during the first 2 broods after exposure, whereas AgNO only had significant effects on the first brood. It is possible that AgNPs have longer adverse effects than AgNO on the reproduction of Daphnia. The present study identified the sensitive site of AgNP toxic action in daphnids and documented the extent of maternal transfer and the significant influence of AgNPs on the reproduction of daphnids. Environ Toxicol Chem 2017;36:3359-3366. © 2017 SETAC.

Exposure medium and particle ageing moderate the toxicological effects of nanomaterials to Daphnia magna over multiple generations: a case for standard test review?

“…Rapid transformation has also been heavily demonstrated in the literature in a variety of different media Environmental Science: Nano Paper using a range of different surface coated NMs. For example multiple studies have focused on determining the physicochemical changes undergone by Ag NPs during and after their use, 26 assessments of their stability in various test media, 7,27 fate/transformations 28 and bioavailability. 29…”

Multigenerational Exposures of Daphnia Magna to Pristine and Aged Silver Nanoparticles: Epigenetic Changes and Phenotypical Ageing Related Effects

“…To address the possible environmental impacts of Ag NPs, multiple studies have focused on determining the physicochemical changes undergone by Ag NPs during and after their use, [ 5 ] assessing stability, [ 6,7 ] fate/transformations, [ 8 ] and bioavailability. [ 9 ] Despite this understanding of the environmental transformations undergone by Ag NPs, acute toxicity assessments of Ag NPs with various test organisms [ 10–13 ] have mainly determined the effects of only the “pristine” Ag NPs.…”

Changes in protein expression in mussels Mytilus galloprovincialis dietarily exposed to PVP/PEI coated silver nanoparticles at different seasons

“…Toxic effects caused by the exposure to Ag NPs through the water have been widely investigated in different freshwater organisms such as microalgae (Navarro et al, 2008;Ribeiro et al, 2014;Sendra et al, 2017a), daphnids (Ribeiro et al, 2014;Khan et al, 2015;Pakrashi et al, 2017) and zebrafish (Griffit et al, 2009;Ribeiro et al, 2014;Lacave et al, 2017;2018;Orbea et al, 2017). In seawater, studies based on waterborne exposure to Ag NPs have mainly been focused on microalgae (Gambardella et al, 2015;Schiavo et al, 2017;Sendra et al, 2017b) and bivalves (Ringwood et al, 2010;Buffet et al, 2013;Gomes et al, 2013a;2013b;McCarthy et al, 2013;Bebianno et al, 2015;Katsumiti et al, 2015;Jimeno-Romero et al, 2017).…”