Bioabatement to Remove Inhibitors from Biomass-Derived Sugar Hydrolysates

Nichols, Nancy N.; Dien, Bruce S.; Guisado, Gema; López, M.J.

doi:10.1385/abab:121:1-3:0379

Cited by 60 publications

(14 citation statements)

References 20 publications

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“…Dilute-acid hydrolysis has been investigated as one of the fast and easy operations, but this operation formed furans at high concentrations (10). Dilute-acid hydrolysis pretreatment of corn stover with inhibitors composed of organic acid and furans was employed as a model composition (19). Total furans were included at higher concentrations than other inhibitors tested in model compositions.…”

Section: Effect Of Individual Inhibitorsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

Sakai

Tsuchida

Nakamoto

et al. 2007

Appl Environ Microbiol

148

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In cellulosic ethanol production, pretreatment of a biomass to facilitate enzymatic hydrolysis inevitably yields fermentation inhibitors such as organic acids, furans, and phenols. With representative inhibitors included in the medium at various concentrations, individually or in various combinations, ethanol production by Corynebacterium glutamicum R under growth-arrested conditions was investigated. In the presence of various inhibitors, the 62 to 100% ethanol productivity retained by the C. glutamicum R-dependent method far exceeded that retained by previously reported methods.

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Section: Effect Of Individual Inhibitorsmentioning

confidence: 99%

“…Through further optimization, the method employing C. glutamicum strain R-ldhA-pCRA723 may be developed into an efficient ethanol production method without detoxification steps. (19).…”

Section: Effect Of Individual Inhibitorsmentioning

confidence: 99%

Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

Sakai

Tsuchida

Nakamoto

et al. 2007

Appl Environ Microbiol

148

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“…Thus, the technological challenges to improve the fermentability of hydrolysates are significantly decreased. Various physical, chemical and biological detoxification methods have been explored, primarily for acid-pretreated biomass (Berson et al, 2005;DeMancilha and Karim, 2003;Klinke et al, 2004;Nichols et al, 2005). Nevertheless, detoxification is a significant economic burden for large-scale production of cellulosic ethanol.…”

Section: Significance Of the Resultsmentioning

confidence: 99%

Ethanolic fermentation of hydrolysates from ammonia fiber expansion (AFEX) treated corn stover and distillers grain without detoxification and external nutrient supplementation

Lau

Dale

Balan

2007

Biotech & Bioengineering

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External nutrient supplementation and detoxification of hydrolysate significantly increase the production cost of cellulosic ethanol. In this study, we investigated the feasibility of fermenting cellulosic hydrolysates without washing, detoxification or external nutrient supplementation using ethanologens Escherichia coli KO11 and the adapted strain ML01 at low initial cell density (16 mg dry weight/L). The cellulosic hydrolysates were derived from enzymatically digested ammonia fiber expansion (AFEX)-treated corn stover and dry distiller's grain and solubles (DDGS) at high solids loading (18% by weight). The adaptation was achieved through selective evolution of KO11 on hydrolysate from AFEX-treated corn stover. All cellulosic hydrolysates tested (36-52 g/L glucose) were fermentable. Regardless of strains, metabolic ethanol yields were near the theoretical limit (0.51 g ethanol/g consumed sugar). Volumetric ethanol productivity of 1.2 g/h/L was achieved in fermentation on DDGS hydrolysate and DDGS improved the fermentability of hydrolysate from corn stover. However, enzymatic hydrolysis and xylose utilization during fermentation were the bottlenecks for ethanol production from corn stover at these experimental conditions. In conclusion, fermentation under the baseline conditions was feasible. Utilization of nutrient-rich feedstocks such as DDGS in fermentation can replace expensive media supplementation.

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“…The number and identity of these toxic compounds varies with the nature of the raw material and pretreatment conditions (see Klinke et al 2004 for a recent review). There are two approaches to limit the impact of inhibitors on the fermentation process: (i) introduction of additional chemical, physical or biological process steps for removal or inactivation of inhibitors (Ló pez et al 2004;Mussatto and Roberto 2004;Nichols et al 2005;Palmqvist et al 1997;Palmqvist and Hahn-Hä gerdal 2000) and (ii) improvement of the tolerance of S. cerevisiae to the inhibitors. Physical and chemical methods of detoxification quickly become cost-prohibitive for an industry that operates with narrow profit margins.…”

Section: Inhibitors In Plant Hydrolysatesmentioning

confidence: 99%

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

Maris

Abbott

Bellissimi

et al. 2006

Antonie van Leeuwenhoek

428

276

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Fuel ethanol production from plant biomass hydrolysates by Saccharomyces cerevisiae is of great economic and environmental significance. This paper reviews the current status with respect to alcoholic fermentation of the main plant biomass-derived monosaccharides by this yeast. Wild-type S. cerevisiae strains readily ferment glucose, mannose and fructose via the Embden-Meyerhof pathway of glycolysis, while galactose is fermented via the Leloir pathway. Construction of yeast strains that efficiently convert other potentially fermentable substrates in plant biomass hydrolysates into ethanol is a major challenge in metabolic engineering. The most abundant of these compounds is xylose. Recent metabolic and evolutionary engineering studies on S. cerevisiae strains that express a fungal xylose isomerase have enabled the rapid and efficient anaerobic fermentation of this pentose.L-Arabinose fermentation, based on the expression of a prokaryotic pathway in S. cerevisiae, has also been established, but needs further optimization before it can be considered for industrial implementation. In addition to these already investigated strategies, possible approaches for metabolic engineering of galacturonic acid and rhamnose fermentation by S. cerevisiae are discussed. An emerging and major challenge is to achieve the rapid transition from proof-of-principle experiments under 'academic' conditions (synthetic media, single substrates or simple substrate mixtures, absence of toxic inhibitors) towards efficient conversion of complex industrial substrate mixtures that contain synergistically acting inhibitors.

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Bioabatement to Remove Inhibitors from Biomass-Derived Sugar Hydrolysates

Cited by 60 publications

References 20 publications

Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

Ethanolic fermentation of hydrolysates from ammonia fiber expansion (AFEX) treated corn stover and distillers grain without detoxification and external nutrient supplementation

Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status

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