Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested
            <i>Corynebacterium glutamicum</i>
            R

Sakai, Shinsuke; Tsuchida, Yoshiki; Nakamoto, Hiroka; Okino, Shohei; Ichihashi, Osamu; Kawaguchi, Hideo; Watanabe, Takashi; Inui, Masayuki; Yukawa, Hideaki

doi:10.1128/aem.02880-06

Cited by 148 publications

(52 citation statements)

References 30 publications

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“…The cell concentration was 60 g CDW/L. Data show averages and standard deviations from four independent experiments engineered strains showing superior production performance compared to the previously reported ethanol-producing C. glutamicum ΔldhA-pCRA723 strain that exhibited maximum ethanol titer of 78 g/L and an ethanol yield of 53 % (Inui et al 2004a;Sakai et al 2007). This study also demonstrated that metabolic engineering of C. glutamicum for ethanol production from a sugar mixture and engineered strain CRZ18-pCRB219 exhibited production of high concentration of ethanol (83 g/L) from the sugar mixture.…”

Section: Discussionmentioning

confidence: 96%

“…Nonetheless, this study clearly demonstrates that overexpression of glycolytic genes is a useful way to improve productivity of bioprocesses using oxygen-deprived C. glutamicum. Additionally, the constructed strain, CRZ18-pCRB219, has the potential to be used for the production of ethanol from lignocellulosic biomass under conditions of oxygen deprivation, where it shows high tolerance to inhibitory compounds generally found in lignocellulose hydrolysates (Sakai et al 2007). …”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, oxygen-deprived C. glutamicum cultures are highly tolerant of inhibitory by-products that are formed during the lignocellulosic biomass pretreatment process (Sakai et al 2007). Although wild-type C. glutamicum does not produce ethanol, introduction of genes encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) from Zymomonas mobilis allows C. glutamicum to produce ethanol from glucose under conditions of oxygen deprivation (Inui et al 2004a).…”

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Jojima

Noburyu

Sasaki

et al. 2014

Appl Microbiol Biotechnol

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Recombinant Corynebacterium glutamicum harboring genes for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) can produce ethanol under oxygen deprivation. We investigated the effects of elevating the expression levels of glycolytic genes, as well as pdc and adhB, on ethanol production. Overexpression of four glycolytic genes (pgi, pfkA, gapA, and pyk) in C. glutamicum significantly increased the rate of ethanol production. Overexpression of tpi, encoding triosephosphate isomerase, further enhanced productivity. Elevated expression of pdc and adhB increased ethanol yield, but not the rate of production. Fed-batch fermentation using an optimized strain resulted in ethanol production of 119 g/L from 245 g/L glucose with a yield of 95% of the theoretical maximum. Further metabolic engineering, including integration of the genes for xylose and arabinose metabolism, enabled consumption of glucose, xylose, and arabinose, and ethanol production (83 g/L) at a yield of 90 %. This study demonstrated that C. glutamicum has significant potential for the production of cellulosic ethanol.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Jojima

Noburyu

Sasaki

et al. 2014

Appl Microbiol Biotechnol

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“…It was isolated in an effort to screen for L-glutamate-producing bacteria (Kinoshita et al 2004;Udaka 1960). Since its discovery, C. glutamicum has been widely investigated and applied in industrial production of various amino acids and vitamins (Hermann 2003;Leuchtenberger et al 2005;Becker et al 2009), and recently of bio-based chemicals such as succinate (Okino et al 2008a), lactate (Okino et al 2008b), ethanol (Inui, et al 2004;Sakai et al 2007), 1,4-diaminobutane (Schneider and Wendisch 2010), 1,5-diaminopentane (Mimitsuka et al 2007), pyruvate (Wieschalka et al 2012), and isobutanol (Blombach et al 2011). Due to its industrial importance, the genomes of several strains of C. glutamicum have been sequenced (Kalinowski et al 2003;Ikeda and Nakagawa 2003;Yukawa et al 2007;Lv et al 2011Lv et al , 2012.…”

Section: Introductionmentioning

confidence: 99%

Degradation and assimilation of aromatic compounds by Corynebacterium glutamicum: another potential for applications for this bacterium?

Shen

Zhou

Liu

2012

Appl Microbiol Biotechnol

112

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With the implementation of the well-established molecular tools and systems biology techniques, new knowledge on aromatic degradation and assimilation by Corynebacterium glutamicum has been emerging. This review summarizes recent findings on degradation of aromatic compounds by C. glutamicum. Among these findings, the mycothiol-dependent gentisate pathway was firstly discovered in C. glutamicum. Other important knowledge derived from C. glutamicum would be the discovery of linkages among aromatic degradation and primary metabolisms such as gluconeogenesis and central carbon metabolism. Various transporters in C. glutamicum have also been identified, and they play an essential role in microbial assimilation of aromatic compounds. Regulation on aromatic degradation occurs mainly at transcription level via pathway-specific regulators, but global regulator(s) is presumably involved in the regulation. It is concluded that C. glutamicum is a very useful model organism to disclose new knowledge of biochemistry, physiology, and genetics of the catabolism of aromatic compounds in high GC content Gram-positive bacteria, and that the new physiological properties of aromatic degradation and assimilation are potentially important for industrial applications of C. glutamicum.

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“…Similarly, arabinose utilization was also achieved, whereby the genes araA, araB, and araD were also derived from E. coli [163]. An interesting study recently investigated the tolerance of C. glutamicum to toxic compounds such as furfurals or phenols, typically present in lignocellulosic raw materials [164]. These compounds cause significant inhibition of growth.…”

Section: Utilization Of Alternative Substratesmentioning

confidence: 99%

Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

Wittmann

2010

Biosystems Engineering I

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The Gram-positive soil bacterium Corynebacterium glutamicum was discovered as a natural overproducer of glutamate about 50 years ago. Linked to the steadily increasing economical importance of this microorganism for production of glutamate and other amino acids, the quest for efficient production strains has been an intense area of research during the past few decades. Efficient production strains were created by applying classical mutagenesis and selection and especially metabolic engineering strategies with the advent of recombinant DNA technology. Hereby experimental and computational approaches have provided fascinating insights into the metabolism of this microorganism and directed strain engineering. Today, C. glutamicum is applied to the industrial production of more than 2 million tons of amino acids per year. The huge achievements in recent years, including the sequencing of the complete genome and efficient post genomic approaches, now provide the basis for a new, fascinating era of research -analysis of metabolic and regulatory properties of C. glutamicum on a global scale towards novel and superior bioprocesses.

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Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R

Cited by 148 publications

References 30 publications

Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum

Degradation and assimilation of aromatic compounds by Corynebacterium glutamicum: another potential for applications for this bacterium?

Analysis and Engineering of Metabolic Pathway Fluxes in Corynebacterium glutamicum

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