Bio-mitigation of CO2, calcite formation and simultaneous biodiesel precursors production using Chlorella sp.

Fulke, Abhay B.; Mudliar, Sandeep N.; Yadav, Raju R.; Shekh, Ajam; Srinivasan, Naganandhini; Ramanan, Rishiram; Krishnamurthi, Kannan; Devi, Sivanesan Saravana; Chakrabarti, Tapan

doi:10.1016/j.biortech.2010.06.012

Cited by 97 publications

(33 citation statements)

References 20 publications

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“…As such, there is an increasing industrial interest in using CA as bio-catalyst for carbon sequestration out of flue gas from coal-fired power plants (Saville and Lalonde, 2011). In addition, there is also interest in exploiting CA in algae as a way to capture CO 2 and convert it into biofuels or other valuable products (Fulke et al, 2010;Ramanan et al, 2010). Human carbonic anhydrase II (HCA II) is a suitable candidate for these applications: It is easy and cost-effective to express and purify, from overexpression in Escherichia coli; it has fast kinetic parameters, with a turnover rate of 10 6 s…”

Section: Introductionmentioning

confidence: 99%

Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II

Fisher

Boone

Biswas

et al. 2012

Protein Engineering Design and Selection

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Carbonic anhydrases (CAs) are ubiquitous enzymes that catalyze the reversible hydration/dehydration of carbon dioxide/bicarbonate. As such, there is enormous industrial interest in using CA as a bio-catalyst for carbon sequestration and biofuel production. However, to ensure cost-effective use of the enzyme under harsh industrial conditions, studies were initiated to produce variants with enhanced thermostability while retaining high solubility and catalytic activity. Kinetic and structural studies were conducted to determine the structural and functional effects of these mutations. X-ray crystallography revealed that a gain in surface hydrogen bonding contributes to stability while retaining proper active site geometry and electrostatics to sustain catalytic efficiency. The kinetic profiles determined under a variety of conditions show that the surface mutations did not negatively impact the carbon dioxide hydration or proton transfer activity of the enzyme. Together these results show that it is possible to enhance the thermal stability of human carbonic anhydrase II by specific replacements of surface hydrophobic residues of the enzyme. In addition, combining these stabilizing mutations with strategic active site changes have resulted in thermostable mutants with desirable kinetic properties.

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Section: Introductionmentioning

confidence: 99%

Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II

Fisher

Boone

Biswas

et al. 2012

Protein Engineering Design and Selection

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“…Using a pump to transport the culture solution between fermentor and tube, both the growth of Chlorella sp. USTB-01 and the fixation of CO 2 were much improved in comparison to those obtained with other bioreactors reported [35,36].…”

Section: Heterotrophic Culture Of Chlorella Sp Ustb-01mentioning

confidence: 70%

Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor

Xuan

Yan

Wang

et al. 2011

Front. Environ. Sci. Eng. China

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A promising microalgal strain isolated from fresh water, which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting, was identified as Chlorella sp. USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences. In the heterotrophic batch culture, more than 20.0 g$L -1 of cell dry weight concentration (DWC) of Chlorella sp. USTB-01 was obtained at day 5, and which was used directly to seed the autotrophic culture. A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp. USTB-01 for the fixation of carbon dioxide (CO 2 ). It showed that the autotrophic growth of Chlorella sp. USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g$L -1 was obtained at day 6. The highest CO 2 fixation of 95% appeared on day 1 in the exponential growth phases of Chlorella sp. USTB-01 and 49.8% protein was found in the harvested microalgal cells.

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“…Thus, integrating the production of such co-products with biofuels will provide new insight into improving the production economics of microalgal biodiesel. Microalgae can www.intechopen.com also be used for sequestration of carbon dioxide from industrial flue gases and wastewater treatment by removal of nutrients (Chinnasamy et al 2010;Fulke et al, 2010;Levine et al, 2011;Yang et al, 2011). Coupled with these environment-beneficial approaches, the production potential of microalgae derived biodiesel is desirable.…”

Section: Potential Of Using Microalgae As Biodiesel Feedstocksmentioning

confidence: 99%

Microalgae as Feedstocks for Biodiesel Production

Liu¹,

Huang²,

Chen³

2011

Biodiesel - Feedstocks and Processing Technologies

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Bio-mitigation of CO2, calcite formation and simultaneous biodiesel precursors production using Chlorella sp.

Cited by 97 publications

References 20 publications

Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II

Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II

Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor

Microalgae as Feedstocks for Biodiesel Production

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