Microalgae as Feedstocks for Biodiesel Production

Liu, Jin; Huang, Junchao; Chen, Feng

doi:10.5772/25600

Cited by 30 publications

(23 citation statements)

References 126 publications

(98 reference statements)

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“…Lipid accumulation refers to increased concentration of lipids within the algal cells without consideration of the overall biomass production [34,35]. In this work however the best biomass producers did not correspond to the best lipid producers.…”

Section: Biomass and Lipid Content Of Screened Microalgae Strainsmentioning

confidence: 69%

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

et al. 2015

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Microalgae are photosynthetic microorganisms that can produce lipids, proteins and carbohydrates in large amounts and within short periods of time and these can be processed into both biofuels and other useful commercial products. Due to this reason microalgae are considered as a potential source of renewable energy; and one of the most important decisions in obtaining oil from microalgae is the choice of species. In this study, the potential of Chlorophyceae species isolated from freshwater and soda lakes in Hungary and Romania (Central Europe) were characterized and evaluated by determining their biomass accumulation, lipid productivity, fatty acid profiles, and biodiesel properties besides protein and carbohydrate productivity. Out of nine strains tested, three accumulated more than 40% dry weight of protein, four accumulated more than 30% dry weight of carbohydrate and the strain Chlorella vulgaris LC8 accumulated high lipid content (42.1% ± 2.6%) with a favorable C16-C18 fatty acid profile (77.4%) as well as suitable biodiesel properties of high cetane number (57.3), low viscosity (4.7 mm 2 /s), lower iodine number (75.18 g I2/100 g), relative cloud point (8.8 °C) and negative cold filter plugging point (−6.5 °C). Hence the OPEN ACCESSEnergies 2015, 8 7503 new strain, Chlorella vulgaris LC8 has potential as a feedstock for the production of excellent quality biodiesel.

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Section: Biomass and Lipid Content Of Screened Microalgae Strainsmentioning

confidence: 69%

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

et al. 2015

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“…Algae are recently being explored as a renewable and sustainable feedstock for biofuels production [ 35 ]. However, at commercial level this technology needs more improvements and advancement for producing huge volumes of algal biomass and efficient pretreatment and conversion to bioethanol to compete the prices of fossil fuels [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Enhancing the Feasibility ofMicrocystis aeruginosaas a Feedstock for Bioethanol Production under the Influence of Various Factors

Khan

Lee

Seo

et al. 2016

BioMed Research International

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Microcystis aeruginosa, a freshwater microalga, is capable of producing and accumulating different types of sugars in its biomass which make it a good feedstock for bioethanol production. Present study aims to investigate the effect of different factors increasing growth rate and carbohydrates productivity of M. aeruginosa. MF media (modified BG11 media) and additional ingredients such as aminolevulinic acid (2 mM), lysine (2.28 mM), alanine (1 mM), and Naphthalene acetic acid (1 mM) as cytokine promoted M. aeruginosa growth and sugar contents. Salmonella showed growth-assisting effect on M. aeruginosa. Enhanced growth rate and carbohydrates contents were observed in M. aeruginosa culture grown at 25°C under red LED light of 90 μmolm−2s−1 intensity. More greenish and carbohydrates rich M. aeruginosa biomass was prepared (final OD660 nm = 2.21 and sugar contents 10.39 mM/mL) as compared to control (maximum OD660 nm = 1.4 and sugar contents 3 mM/mL). The final algae biomass was converted to algae juice through a specific pretreatment method. The resulted algae Juice was used as a substrate in fermentation process. Highest yield of bioethanol (50 mM/mL) was detected when Brettanomyces custersainus, Saccharomyces cerevisiae, and Pichia stipitis were used in combinations for fermentation process as compared to their individual fermentation. The results indicated the influence of different factors on the growth rate and carbohydrates productivity of M. aeruginosa and its feasibility as a feedstock for fermentative ethanol production.

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“…doubling time of S. elongatus PCC 2973, Synechococcus PCC 7942, and Synechocystis PCC 6803, is 1.9, 4, and 7-10 h, respectively), representing a potential disadvantage if scaled-up under outdoor conditions. A similar situation is faced for microalgal strains which usually display even slower doubling times [11]. Thus, production costs become a major factor in selecting cyanobacterial versus bacterial as production systems.…”

Section: Introductionmentioning

confidence: 91%

Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

González-Morales¹,

Pacheco-Gutiérrez²,

Ramírez-Rodríguez³

et al. 2020

Preprint

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Background: The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by public and private sectors to develop this field. However, it has been a challenge to develop a viable and cost-effective platform for cultivation of cyanobacteria and microalgae under outdoor conditions. Dealing with contamination caused by bacteria, weedy algae/cyanobacteria and other organisms is a major constraint to establish effective cultivation processes.Results: Here we describe the implementation in the cyanobacterium Synechococcus elongatus PCC 7942 of a phosphorus selective nutrition system to control biological contamination during cultivation. The system is based on metabolic engineering of S. elongatus to metabolize phosphite, a phosphorus source not normally metabolized by most organisms, by expressing a bacterial phosphite oxidoreductase (PtxD). Engineered S. elongatus strains expressing PtxD grow at a similar rate on media supplemented with phosphite as the non-transformed control supplemented with phosphate. We show that when grown in media containing phosphite as the sole phosphorus source in glass flasks, the engineered strain was able to grow and outcompete biological contaminants even when the system was intentionally inoculated with natural competitors isolated from an irrigation canal. The PtxD/phosphite system was successfully used for outdoor cultivation of engineered S. elongatus in 100 L cylindrical reactors and 1,000 L raceway ponds, under non-axenic conditions and without the need of sterilizing containers and media. Finally, we also show that the PtxD/phosphite system can be used as selectable marker for S. elongatus PCC 7942 transgenic strains selection, eliminating the need of antibiotic resistance genes. Conclusions: Our results suggest that the PtxD/phosphite system is a stable and sufficiently robust strategy to control biological contaminants without the need of sterilization or other complex aseptic procedures. Our data shows that the PtxD/phosphite system can be used as selectable marker and allows production of the cyanobacterium S. elongatus PCC 7942 in non-axenic outdoor reactors at lower cost, which in principle should be applicable to other cyanobacteria and microalgae engineered to metabolize phosphite.

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Microalgae as Feedstocks for Biodiesel Production

Cited by 30 publications

References 126 publications

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

Screening and Evaluation of Some Green Algal Strains (Chlorophyceae) Isolated from Freshwater and Soda Lakes for Biofuel Production

Enhancing the Feasibility ofMicrocystis aeruginosaas a Feedstock for Bioethanol Production under the Influence of Various Factors

Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

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