Bio-mechanical characterization of a CAD/CAM PMMA resin for digital removable prostheses

Pagano, Stefano; Lombardo, Guido; Caponi, Silvia; Costanzi, Egidia; Michele, Alessandro Di; Bruscoli, Stefano; Xhimitiku, Iva; Coniglio, Maddalena; Valenti, Chiara; Mattarelli, Maurizio; Rossi, Gianluca; Cianetti, Stefano; Marinucci, Lorella

doi:10.1016/j.dental.2020.11.003

Cited by 31 publications

(25 citation statements)

References 46 publications

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“…Currently, CAD/CAM techniques have found widespread application for all types of prostheses—fixed, partial, and completely removable, with different types of ceramic and polymer-based materials [ 10 , 15 , 16 , 86 ]. Future studies comparing the fit accuracy and fracture resistance of the latest monolithic translucent Zi (partially and fully stabilized zirconia), fully crystallized ZLS, and nano-ceramic materials with the monolithic Y-TZP, LDS, and ZLS pre-crystallized glass ceramic materials are required to test the efficacy of the new materials for different types of indirect fixed restorations.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Marginal/Internal Fit and Fracture Load of Monolithic Zirconia and Zirconia Lithium Silicate (ZLS) CAD/CAM Crown Systems

2021

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Fit accuracy and fracture strength of milled monolithic zirconia (Zi) and zirconia-reinforced lithium silicate (ZLS) crowns are important parameters determining the success of these restorations. This study aimed to evaluate and compare the marginal and internal fit of monolithic Zi and ZLS crowns, along with the fracture load, with and without mechanical aging. Thirty-two stone dies acquired from a customized master metal molar die were scanned, and ceramic crowns (16 Zi Ceramill Zolid HT+ and 16 ZLS Vita Suprinity) were designed and milled. Absolute marginal discrepancies (AMD), marginal gaps (MG), and internal gaps (IG) of the crowns, in relation to the master metal die, were evaluated using x-ray nanotomography (n = 16). Next, thirty-two metal dies were fabricated based on the master metal die, and crowns (16 Zi; 16 ZLS) cemented and divided into four groups of eight each; eight Zi with mechanical aging (MA), eight Zi without mechanical aging (WMA), eight ZLS (MA), and eight ZLS (WMA). Two groups of crowns (Zi-MA; ZLS-MA) were subjected to 500,000 mechanical cycles (200 ± 50 N, 10 Hz) followed by axial compressive strength testing of all crowns, until failure, and the values were recorded. Independent sample t tests (α = 0.05) revealed no significant differences between Zi and ZLS crowns (p > 0.05); for both internal and marginal gaps, however, there were significant differences in AMD (p < 0.005). Independent samples Mann–Whitney U and Kruskal–Wallis tests revealed significant differences between the two materials, Zi and ZLS, regardless of fatigue loading, and for the individual material groups based on aging (α = 0.05). Multiple comparisons using Bonferroni post-hoc analysis showed significant differences between Zi and ZLS material groups, with or without aging. Within the limitations of this study, the ZLS crown fit was found to be on par with Zi, except for the AMD parameter. As regards fracture resistance, both materials survived the normal range of masticatory forces, but the Zi crowns demonstrated greater resistance to fracture. The monolithic Zi and ZLS crowns seem suitable for clinical application, based on the fit and fracture strength values obtained.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Marginal/Internal Fit and Fracture Load of Monolithic Zirconia and Zirconia Lithium Silicate (ZLS) CAD/CAM Crown Systems

2021

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“…Cells were thrice washed with PBS, fixed in 2.5% glutaraldehyde in phosphate‐buffered saline PBS for 1 h at room temperature, thrice washed with distilled water, dehydrated stepwise in ethanol and dried at room temperature. 28 After critical point drying, using the Freon method, samples were splutter‐metallized with chromium (8 nm) and viewed under a Field Emission Gun SEM LEO 1525 (ZEISS, Jena Germany). The acceleration voltage was 5 KV and images were obtained using an in‐lens detector.…”

Section: Methodsmentioning

confidence: 99%

Heat‐not‐burn tobacco (IQOS), oral fibroblasts and keratinocytes: cytotoxicity, morphological analysis, apoptosis and cellular cycle. An in vitro study

Pagano

Negri

Coniglio

et al. 2021

J of Periodontal Research

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Objectives The aim of this work is to investigate the biological effects of IQOS smoking on human gingival fibroblasts and human keratinocytes analysing cell viability, morphology, migration, apoptosis and cell cycle. Background Electronic cigarettes and tobacco heating systems have been marketed to reduce smoking damages caused by combustion. Methods Human gingival fibroblasts and human keratinocytes viability was determined by a colorimetric assay measuring mitochondrial dehydrogenase activity (MTT assay); after an in vitro exposure of 24 h, cell morphology was analysed with scanning electron microscope and cell migration was tested by Scratch assay, a method to mimic the migration of the cells during wound healing in vivo. Apoptosis and cell cycle were analysed with flow cytometry, and the expression of related genes (p53, Bcl2, p16 and p21) was indagated using real‐time polymerase chain reaction. Results IQOS extracts increased both cell viability (23%‐41% with fibroblasts and 30%‐79% with keratinocytes) and migration. No morphological alterations were observed. IQOS extracts did not induced an increase in cell death, but rose the number of S‐ and G2/M‐phase cells. IQOS extracts also significantly increased p53 expression by fibroblasts (undiluted and 6.25% dilution, 2‐ and 3.6‐fold higher, respectively) and reduced both Bcl2 (about two‐ and fivefold, respectively) and p21 expressions (about twofold with both extracts), while on keratinocytes both undiluted and 6.25% dilution extracts increased Bcl2 expression (about four‐ and threefold higher, respectively) and reduced p53 expression (about two‐ and fivefold, respectively). Conclusion IQOS smoke seemed to induce proliferation as highlighted by a viability assay, and migration and cell cycle analysis. The increased cell proliferation induced by IQOS devices must be carefully investigated for its possible clinical effects on oral cell populations.

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“…However, the analysis was limited in that it evaluated the specimens in settings that differ from the oral cavity environment with regard to alterations in temperature and pH and the absence of natural saliva, which contains a variety of species of microorganisms. Nanoparticlemodified denture base material displayed improved physical and mechanical properties in the present study, but further research investigating the addition of nanoparticles to computer-aided design and computer-aided manufacturing (CAD/CAM) prepolymerized disks should be performed in order to solidify this method as a promising technique [37].…”

Section: Discussionmentioning

confidence: 86%

In Vitro Assessment of Artificial Aging on the Antifungal Activity of PMMA Denture Base Material Modified with ZrO2 Nanoparticles

Hamid

Alghamdi

Alshahrani

et al. 2021

International Journal of Dentistry

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The antifungal effect of zirconium dioxide nanoparticles (ZrO2NPs) incorporated into denture base material has been inadequately investigated; additionally, to the authors’ knowledge, no studies have assessed the influence of artificial aging on the antifungal activity of these particles. Methodology. Heat-polymerized acrylic resin disks were fabricated and divided into four groups (0%, 1%, 2.5%, and 5% ZrO2NPs by weight). Antifungal activity was assessed using the direct culture and disk diffusion methods. Surface roughness and contact angles were measured using a profilometer and a goniometer, respectively. The artificial aging procedure was performed by repeating all tests at 7, 14, and 30 days following 2 rounds of thermocycling. Data were analyzed using ANOVA and Tukey’s post-hoc test ( p < 0.05 ). Results. The addition of ZrO2NPs significantly decreased the adhesion of Candida albicans with and without artificial aging procedures ( p < 0.001 ), while the disk diffusion methods did not reveal inhibition zones. ZrO2NP-modified specimens displayed significantly higher surface roughness compared to specimens in the control group ( p < 0.05 ) and showed the same behaviors with artificial aging procedures. The contact angle was significantly decreased in all modified groups in comparison to the control group ( p < 0.05 ). Conclusion. The addition of ZrO2NPs to polymethylmethacrylate denture base material reduced the adhesion of Candida albicans with a long-term antifungal effect. With the addition of ZrO2NPs, contact angles were decreased and surface roughness was increased; 1% was the most appropriate concentration. Clinical significance. The addition of ZrO2NPs to denture base material confers a long-term antifungal effect and could be used as a possible method for preventing and treating denture stomatitis.

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Bio-mechanical characterization of a CAD/CAM PMMA resin for digital removable prostheses

Cited by 31 publications

References 46 publications

Evaluation of Marginal/Internal Fit and Fracture Load of Monolithic Zirconia and Zirconia Lithium Silicate (ZLS) CAD/CAM Crown Systems

Evaluation of Marginal/Internal Fit and Fracture Load of Monolithic Zirconia and Zirconia Lithium Silicate (ZLS) CAD/CAM Crown Systems

Heat‐not‐burn tobacco (IQOS), oral fibroblasts and keratinocytes: cytotoxicity, morphological analysis, apoptosis and cellular cycle. An in vitro study

In Vitro Assessment of Artificial Aging on the Antifungal Activity of PMMA Denture Base Material Modified with ZrO2 Nanoparticles

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