Bio‐Inspired Cryo‐Ink Preserves Red Blood Cell Phenotype and Function During Nanoliter Vitrification

Assal, Rami El; Güven, Sinan; Gürkan, Umut A.; Gözen, İrep; Shafiee, Hadi; Dalbeyler, Sedef; Abdalla, Noor; Thomas, Gawain; Fuld, Wendy; Illigens, Ben; Estanislau, Jessica; Khoory, Joseph; Kaufman, Richard M.; Zylberberg, Claudia; Lindeman, Neal; Wen, Qi; Ghiran, Ionita; Demirci, Utkan

doi:10.1002/adma.201400941

Cited by 38 publications

(39 citation statements)

References 58 publications

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“…Various 3-D culturing techniques ( e.g., hanging drop, microfluidic systems, bioprinting, assembly, spinner flasks, and rotary system) have been successfully used to generate 3-D tumor models192021222324252627282930313233. For example, hanging drop approach has been increasingly used to generate 3-D models due its simplicity; however, it is still challenging to use this method to provide long-term cultures.…”

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confidence: 99%

3-D Microwell Array System for Culturing Virus Infected Tumor Cells

Assal

Gürkan

Chen

et al. 2016

Sci Rep

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Cancer cells have been increasingly grown in pharmaceutical research to understand tumorigenesis and develop new therapeutic drugs. Currently, cells are typically grown using two-dimensional (2-D) cell culture approaches, where the native tumor microenvironment is difficult to recapitulate. Thus, one of the main obstacles in oncology is the lack of proper infection models that recount main features present in tumors. In recent years, microtechnology-based platforms have been employed to generate three-dimensional (3-D) models that better mimic the native microenvironment in cell culture. Here, we present an innovative approach to culture Kaposi’s sarcoma-associated herpesvirus (KSHV) infected human B cells in 3-D using a microwell array system. The results demonstrate that the KSHV-infected B cells can be grown up to 15 days in a 3-D culture. Compared with 2-D, cells grown in 3-D had increased numbers of KSHV latency-associated nuclear antigen (LANA) dots, as detected by immunofluorescence microscopy, indicating a higher viral genome copy number. Cells in 3-D also demonstrated a higher rate of lytic reactivation. The 3-D microwell array system has the potential to improve 3-D cell oncology models and allow for better-controlled studies for drug discovery.

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confidence: 99%

3-D Microwell Array System for Culturing Virus Infected Tumor Cells

Assal

Gürkan

Chen

et al. 2016

Sci Rep

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“…The simple microfluidic device presented in this study can be further developed for mechanical characterization of single oocytes by measuring the hydrodynamic pressure differences, which is similar in operating principles to a microfluidic system for evaluating red blood cell deformability. 34 Besides, this microfluidic device can be used to study spindle damage in other cell types by changing the size of the constricted segment to be smaller than the size of target cells.…”

Section: Resultsmentioning

confidence: 99%

Deformation of a single mouse oocyte in a constricted microfluidic channel

Luo

Güven

Gözen

et al. 2015

Microfluid Nanofluid

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Single oocyte manipulation in microfluidic channels via precisely controlled flow is critical in microfluidic-based in vitro fertilization. Such systems can potentially minimize the number of transfer steps among containers for rinsing as often performed during conventional in vitro fertilization and can standardize protocols by minimizing manual handling steps. To study shape deformation of oocytes under shear flow and its subsequent impact on their spindle structure is essential for designing microfluidics for in vitro fertilization. Here, we developed a simple yet powerful approach to (i) trap a single oocyte and induce its deformation through a constricted microfluidic channel, (ii) quantify oocyte deformation in real-time using a conventional microscope, and (iii) retrieve the oocyte from the microfluidic device to evaluate changes in their spindle structures. We found that oocytes can be significantly deformed under high flow rates, e.g., 10 μl/min in a constricted channel with a width and height of 50 and 150 μm, respectively. Oocyte spindles can be severely damaged, as shown here by immunocytochemistry staining of the microtubules and chromosomes. The present approach can be useful to investigate underlying mechanisms of oocyte deformation exposed to well-controlled shear stresses in microfluidic channels, which enables a broad range of applications for reproductive medicine.

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“…Bioprinting has emerged as a promising cell and biomaterial patterning strategy that uses an additive manufacturing method to build functional tissue mimicking constructs [5–10]. However, utilizing bioprinting in biomanufacturing industry has been hampered by several limitations, including the inability to reconstitute the intrinsic tissue morphology and function [11].…”

Section: Printing 3d Tissue Constructs With Decellularized Extracellumentioning

confidence: 99%