Bio-control activity of Pichia anomala supplemented with chitosan against Penicillium expansum in postharvest grapes and its possible inhibition mechanism

Godana, Esa Abiso; Yang, Qiya; Wang, Kaili; Zhang, Hongyin; Zhang, Xiaoyun; Zhao, Lina; Abdelhai, Mandour H.; Legrand, Ngolong Ngea Guillaume

doi:10.1016/j.lwt.2020.109188

Cited by 42 publications

(17 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efforts to control the mold population in vineyards are usually directed to B. cinerea [ 6 , 36 , 65 ], Alternaria alternaria [ 66 ], mycotoxigenic species of Aspergillus section Nigri [ 41 , 67 ], and P. expansum [ 44 , 46 ]. However, the relevance of other species in the pathogenesis and spoilage of grapes deserves attention [ 5 , 47 ]).…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus amyloliquefaciens , B. pumilus, and Lactobacillus plantarum [ 37 , 38 ] among bacteria; yeast species as A. pullulans , Kluyveromyces thermotolerans, M. pulcherrima, H. uvarum [ 39 , 40 , 41 , 42 ]; and filamentous fungi as Acremonium cephalosporium [ 43 ] were efficient antagonists against A. carbonarius , and A. nigri ; besides of producing a remarkable reduction of OTA accumulation on grapes. Works directed to control Penicillium development in grapes and vineyards by antagonistic agents are limited and mainly focused on P. expansum [ 44 , 45 , 46 ] and to a lesser extent to Penicillium commune [ 39 ], Penicillium digitatum [ 47 ] are other species of this genus. Nevertheless, the biological control of P. glabrum has not been still addressed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Control of Penicillium glabrum by Indigenous Antagonistic Yeast from Vineyards

Cabañas

Hernández

Martínez

et al. 2020

Foods

View full text Add to dashboard Cite

Biocontrol is one of the most promising alternatives to synthetic fungicides for food preservation. Botrytis cinerea, Alternaria alternata, and Aspergillus section Nigri are the most concerning pathogens for grape development. However, frequently, other species, such as Penicillium glabrum in this study, are predominant in spoiled bunches. In this work, 54 native yeasts from vineyards were screened by direct confrontation in potato dextrose agar plates as antagonists against P. glabrum. Isolates of Pichia terricola, Aureobasidium pullulans, and Zygoascus meyerae were selected for their antagonist activity in vitro, plus isolates of Pichia kudriavzevii, Hormonema viticola, and Hanseniaspora uvarum were used as negative controls. However, in vivo, confrontations in wounded grapes showed disagreement with direct confrontation in vitro. P. terricola, P. kudriavzevii, H. viticola, Z. meyerae, and H. uvarum significantly reduced the incidence of P. glabrum on grapes. Nevertheless, P. terricola, H. viticola, and H. uvarum themselves spoiled the wounded grapes. Inhibitions were associated with different mechanisms such as the production of volatile organic compounds (VOCs), lytic enzymes, biofilm formation, and competition for nutrients. The isolates of P. kudriavzevii L18 (a producer of antifungal VOCs which completely inhibited the incidence of P. glabrum) and Z. meyerae L29 (with pectinase, chitinase and β-glucanase activity and biofilm formation which reduced 70% of the incidence of P. glabrum) are proposed as suitable biocontrol agents against P. glabrum.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Control of Penicillium glabrum by Indigenous Antagonistic Yeast from Vineyards

Cabañas

Hernández

Martínez

et al. 2020

Foods

View full text Add to dashboard Cite

show abstract

“…Red Globe) were obtained from a vineyard in Zhenjiang, Jiangsu Province, China. The fruits were chosen and prepared according to our previous research method [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…P. anomala (strain TL0903) was used for all the experiment, and fresh suspension was prepared according to our previous research method [ 13 ]. Chitosan (90% deacetylation) was bought from Sangon Biotech Co., Ltd. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, the microbial and antifungal characteristics of P. anomala induced with chitosan against blue mold disease of table grapes caused by Penicillium expansum were studied through physical, biochemical, and microscopic techniques. It was also confirmed that chitosan (1% w / v ) is the best inducer of P. anomala to control blue mold diseases of table grapes [ 13 ]. However, transcriptome techniques can offer a deeper understanding of how the antagonistic yeast can control or inhibit the pathogenic fungi and other major postharvest diseases of the fruit.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to Pichia anomala Induced with Chitosan

Godana

et al. 2021

Foods

Self Cite

View full text Add to dashboard Cite

Transcriptome analysis (TA) was conducted to characterize the transcriptome changes in postharvest disease-related genes of table grapes following treatment with Pichia anomala induced with chitosan (1% w/v). In the current study, the difference in the gene expression of table grapes after treatment with P. anomala induced with chitosan and that of a control group was compared 72 h post-inoculation. The study revealed that postharvest treatment of table grapes with P. anomala induced with chitosan could up-regulate genes that have a pivotal role in the fruit’s disease defense. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) results also confirmed that GO terms and the KEGG pathways, which have pivotal roles in plant disease resistance, were significantly enriched. The up-regulated genes of the treatment group have a unique function in the fruit’s disease resistance compared to the control group. Generally, most genes in the plant–pathogen interaction pathway; the plant Mitogen-activated protein kinase (MAPK) signaling pathway; the plant hormone signal transduction pathway; the pathway of glutathione metabolism; the pathway of phenylalanine, tyrosine, and tryptophan biosynthesis; and the pathway of flavonoid biosynthesis were all up-regulated. These up-regulations help the fruit to synthesize disease-resistant substances, regulate the reactive oxygen species (ROS), enhance the fruit cell wall, and enrich hormone signal transduction during the pathogen’s attack. This study is useful to overcome the lags in applying transcriptomics technology in postharvest pathology, and will provide insight towards developing other alternative methods to using bio-pesticides to control postharvest diseases of perishables.

show abstract

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Dhanasekaran

Yang

Godana

et al. 2021

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND In the process of biological control, the antagonistic yeasts contend with various stresses that negatively influence yeasts' biocontrol efficiency. In the current study, we investigated the effect of trehalose supplementation on the biocontrol efficiency and oxidative stress tolerance of Sporidiobolus pararoseus Y16. RESULTS S. pararoseus Y16, an antagonistic yeast cultured in trehalose supplemented medium, exhibited better biocontrol efficiency against Penicillium expansum and Aspergillus tubingensis in table grapes. Trehalose‐treated S. pararoseus Y16 cells showed good proliferation efficiency and oxidative stress tolerance than untreated cells. Increased β‐1,3‐glucanase, catalase, superoxide dismutase activity, and low protein carbonylation were observed in trehalose‐amended S. pararoseus Y16 upon H2O2 exposure. The RNA sequencing results indicated that trehalose significantly altered the transcriptome of S. pararoseus Y16. The GO, KEGG, and COG annotations revealed that the differentially regulated genes corresponded to the various biological process of the yeast. CONCLUSION Our findings suggested that trehalose use could enhance the biocontrol efficiency and oxidative stress tolerance of S. pararoseus Y16. Trehalose supplementation altered the transcriptome of S. pararoseus Y16, particularly the genes that correspond to amino acid metabolism, nucleotide metabolism, and protein modification. Thereby the oxidative stress tolerance and biological control efficiency of S. pararoseus Y16 was enhanced by trehalose. © 2021 Society of Chemical Industry.

show abstract

Bio-control activity of Pichia anomala supplemented with chitosan against Penicillium expansum in postharvest grapes and its possible inhibition mechanism

Cited by 42 publications

References 42 publications

Control of Penicillium glabrum by Indigenous Antagonistic Yeast from Vineyards

Control of Penicillium glabrum by Indigenous Antagonistic Yeast from Vineyards

Transcriptome Characterization and Expression Profiles of Disease Defense-Related Genes of Table Grapes in Response to Pichia anomala Induced with Chitosan

Trehalose supplementation enhanced the biocontrol efficiency of Sporidiobolus pararoseusY16 through increased oxidative stress tolerance and altered transcriptome

Contact Info

Product

Resources

About