(Bio-)chemical Sensing and Imaging by LAPS and SPIM

Yoshinobu, Tatsuo; Krause, Steffi; Miyamoto, Ko Ichiro; Werner, Carl Frederik; Poghossian, Arshak; Wagner, Torsten; Schöning, Michael J.

doi:10.1007/5346_2017_22

Cited by 2 publications

(8 citation statements)

References 98 publications

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“…The scanning LAPS measurement setup used in this study was described in detail in our previous reports. [1,6,23,24] The schematic diagram of the setup is shown in Figure 1a. In brief, the LAPS chip as described in Section 2.1 was assembled in a measurement cell.…”

Section: Scanning Laps Measurement Setupmentioning

confidence: 99%

“…Local variations in the surface potential influence the amplitude of the photocurrent in the space‐charge region. In this way, by changing the location of the light source, the generated photocurrent in the particular sensor area can be read out …”

Section: Introductionmentioning

confidence: 99%

“…In this way, by changing the location of the light source, the generated photocurrent in the particular sensor area can be read out. [1][2][3][4][5] Typical current versus voltage plots (I/V curves) can be obtained by sweeping the applied bias voltage and simultaneously illuminating an area of the semiconductor. As shown in Figure 1b, depending on the local analyte concentration in the solution, the I/V curve moves along the horizontal axis.…”

Section: Introductionmentioning

confidence: 99%

“…More details regarding the measurement principle can be found elsewhere. [1,3,6] The spatial scanning principle of the LAPS also has a great potential for chemical imaging applications, especially where biological substances such as animal cells or microorganisms are concerned. The metabolic activity of the cultured cells on the sensor surface can be detected by means of marker molecules such as H þ ions at the interface between the sensor surface and the cells.…”

Section: Introductionmentioning

confidence: 99%

“…Potentiometric sensors based on a semiconductor‐insulator field‐effect structure play an important role in label‐free (bio‐)chemical sensing . The light‐addressable potentiometric sensor (LAPS) is an important subtype of this kind of sensors, which is able to detect the variations of the surface charge in a spatially resolved manner .…”

Section: Introductionmentioning

confidence: 99%

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Effect of Plasma Treatment on the Sensor Properties of a Light‐Addressable Potentiometric Sensor (LAPS)

Özsoylu

Kızıldağ

Schöning

et al. 2019

Physica Status Solidi (a)

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A light‐addressable potentiometric sensor (LAPS) is a field‐effect‐based (bio‐) chemical sensor, in which a desired sensing area on the sensor surface can be defined by illumination. Light addressability can be used to visualize the concentration and spatial distribution of the target molecules, e.g., H+ ions. This unique feature has great potential for the label‐free imaging of the metabolic activity of living organisms. The cultivation of those organisms needs specially tailored surface properties of the sensor. O2 plasma treatment is an attractive and promising tool for rapid surface engineering. However, the potential impacts of the technique are carefully investigated for the sensors that suffer from plasma‐induced damage. Herein, a LAPS with a Ta2O5 pH‐sensitive surface is successfully patterned by plasma treatment, and its effects are investigated by contact angle and scanning LAPS measurements. The plasma duration of 30 s (30 W) is found to be the threshold value, where excessive wettability begins. Furthermore, this treatment approach causes moderate plasma‐induced damage, which can be reduced by thermal annealing (10 min at 300 °C). These findings provide a useful guideline to support future studies, where the LAPS surface is desired to be more hydrophilic by O2 plasma treatment.

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Section: Scanning Laps Measurement Setupmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Plasma Treatment on the Sensor Properties of a Light‐Addressable Potentiometric Sensor (LAPS)

Özsoylu

Kızıldağ

Schöning

et al. 2019

Physica Status Solidi (a)

Self Cite

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A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria

Dantism

Röhlen

Wagner

et al. 2019

Sensors

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Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.

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(Bio-)chemical Sensing and Imaging by LAPS and SPIM

Cited by 2 publications

References 98 publications

Effect of Plasma Treatment on the Sensor Properties of a Light‐Addressable Potentiometric Sensor (LAPS)

Effect of Plasma Treatment on the Sensor Properties of a Light‐Addressable Potentiometric Sensor (LAPS)

A LAPS-Based Differential Sensor for Parallelized Metabolism Monitoring of Various Bacteria

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