Bio-based succinic acid sample preparation and derivatization procedure optimisation for gas chromatography-mass spectrometry analysis

Jarukas, Laurynas; Kamarauskaitė, Justina; Marksa, Mindaugas; Trumbeckaitė, Sonata; Banienė, Rasa; Ivanauskas, Liudas

doi:10.15587/2519-4852.2018.135132

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…Due to the polar nature of amino acids, derivatization is required prior to GC analysis [45]. A higher sensitivity can be reached during the analysis of amino acids for GC-MS by using derivatization agents, such as N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA).…”

Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Amino Acids Content in the Dry Extracts of Juno bucharica, Gladiolus Hybrid Zefir, Iris Hungarica, Iris Variegata and Crocus Sativus Raw Materials of Ukrainian Flora

et al. 2020

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The aim of this research was the comparative study of the amino acids content in the dry extracts of Iridaceae plants of Ukrainian flora: Juno bucharica leaves and corms, Gladiolus hybrid Zefir leaves, Iris hungarica and Iris variegata rhizomes, and Crocus sativus stigmas, flowers, leaves and corms. A gas chromatography–mass spectrometry (GC–MS) method has been used. Separation of amino acids in the samples was carried out using a Shimadzu GC-MS-QP2010 equipped with an Rxi-5ms (Restek Corporation capillary column (30 m long, 0.25 mm outer diameter and 0.25 µm) with a liquid stationary phase (5% diphenyl and 95% polysiloxane) after derivatization with N-(t-butyldimethylsilyl)-N109 methyltrifluoroacetamide (MTBSTFA) reagent. The results obtained have shown that extracts from the aerial parts of plants investigated have a higher amino acid content and more diverse composition than the underground organs. Experimental data showed that Crocus leaves and Juno leaves extracts contain the highest general content of amino acids—31.99 mg/g and 14.65 mg/g respectively. All samples showed a high content of L-pyroglutamic acid (0.33–12.35 mg/g). Moreover, Crocus leaves and Juno leaves extracts had the most suitable amino acids composition and are prospective for further pharmacological studies.

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Section: Discussionmentioning

confidence: 99%

Comparative Investigation of Amino Acids Content in the Dry Extracts of Juno bucharica, Gladiolus Hybrid Zefir, Iris Hungarica, Iris Variegata and Crocus Sativus Raw Materials of Ukrainian Flora

et al. 2020

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“…Appropriate to the polar nature of amino acids, derivatization is required prior to HPLC analysis (Jarukas et al 2018). Higher sensitivity can be reached during the analysis of amino acids for the HPLC method by using derivatization agents, such as FMOC (9-fluorenylmethyl chloroformate) and OPA (o-phthalaldehyde).…”

Section: Resultsmentioning

confidence: 99%

Investigation of amino acids content in the herb and tubers of Stachys sieboldii

Marchyshyn¹,

Mysula²,

Kishchuk³

et al. 2022

PHAR

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The aim of this research was the comparative study of the content of the amino acids in the herb and tubers of Stachys sieboldii. The study of the amino acid composition of the raw materials was carried out using high-performance liquid chromatography (HPLC). The results obtained have shown that the aerial parts of plants investigated have higher amino acid content than the underground organs. Free and bound L-aspartic acid, L-proline, and L-phenylalanine were present in the analyzed samples in the greatest amount. Moreover, L-cysteine was found only in Stachys sieboldii tubers in amounts (8.11 mg/g). This research established that Stachys sieboldii herb and tubers have the most suitable amino acids composition and are prospective for further pharmacological studies.

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“…Owing to its applications in agriculture, chemical and food, pharmaceutical industries, pyruvic acid demand is ever increasing . Succinic acid is the predecessor of a wide range of bio-compounds, particularly it is important in accumulation of mitochondrial metabolite succinate and during ischemia controls reperfusion injury through mitochondrial reactive oxygen production (Jarukas et al 2018). The followed ones isocitric and citric acids, the content of which were 12.88 mg/g and 8.71 mg/g respectively in the raw material.…”

Section: Resultsmentioning

confidence: 99%

Analysis of carboxylic acids of Crambe cordifolia Steven

Marchyshyn¹,

Slobodianiuk²,

Budniak³

et al. 2021

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Crambe cordifolia Steven is a perennial herb and contains many biologically active substances, including amino acids, quercetin and glycosides of kaempferol. In continuation of the investigation of these plant compounds, it is advisable to study the qualitative composition and quantitative contents of carboxylic acids. Using a HPLC method the quantitative content of the following organic acids was identified and determined: pyruvic (40.66 mg/g), isocitric (12.88 mg/g), citric (8.71 mg/g), succinic (38.03 mg/g) and malic (0.75 mg/g). Among fatty acids the saturated and unsaturated acids were determined by the GC/MS method. The content of polyunsaturated fatty acids of the total fatty acids was 56.97%, saturated – 38.53% and monounsaturated – 4.50%. Linolenic and palmitic acids dominated among the determined 7 fatty acids, their content was 9.68 mg/g (47.87%) and 4.88 mg/g (24.14%). The results of the study show that Crambe cordifolia Steven leaves is a source of carboxylic acids.

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Bio-based succinic acid sample preparation and derivatization procedure optimisation for gas chromatography-mass spectrometry analysis

Cited by 5 publications

References 7 publications

Comparative Investigation of Amino Acids Content in the Dry Extracts of Juno bucharica, Gladiolus Hybrid Zefir, Iris Hungarica, Iris Variegata and Crocus Sativus Raw Materials of Ukrainian Flora

Comparative Investigation of Amino Acids Content in the Dry Extracts of Juno bucharica, Gladiolus Hybrid Zefir, Iris Hungarica, Iris Variegata and Crocus Sativus Raw Materials of Ukrainian Flora

Investigation of amino acids content in the herb and tubers of Stachys sieboldii

Analysis of carboxylic acids of Crambe cordifolia Steven

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