Comparative Investigation of Amino Acids Content in the Dry Extracts of Juno bucharica, Gladiolus Hybrid Zefir, Iris Hungarica, Iris Variegata and Crocus Sativus Raw Materials of Ukrainian Flora

Мykhailenko, Olha; Ivanauskas, Liudas; Безрук, Иван; Lesyk, Roman; Georgiyants, Victoriya

doi:10.3390/scipharm88010008

Cited by 17 publications

(17 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was dissolved in a 200 µL mixture of acetonitrile and MTBSTFA (1:1), then heated in a glycerol bath and subjected to amino acid gas chromatography analysis. The gas chromatography method has been previously described by Mykhailenko et al [22] and Jarukas et al [23]. Briefly, the SHIMADZU GC-MS-QP2010 system and Rxi-5 ms (Restek Corporation, Bellefonte, PA, USA) capillary column (30 m, 0.25 mm outer diameter, 0.25 µm) were applied.…”

Section: Amino Acids and Carboxylic Acids Assaysmentioning

confidence: 99%

“…The derivatization procedure is highly dependent on the solvent used. Therefore, in our previous work [22,23], we developed the GC-MS method for organic acid investigation. Different times, temperatures and the effect of the reagent amount on the derivatization process were tested.…”

Section: Gc-ms Analysis Of Amino Acids and Short-chained And Long-chain Carboxylic Acidsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Phytochemical Components of Crocus sativus Leaves: A New Attractive By-Product

et al. 2021

Self Cite

View full text Add to dashboard Cite

Crocus sativus L. is one of the world’s most famous saffron production crops and its enormous by-products, such as leaves, are an excellent source of bioactive compounds with potential nutritional applications. The total phenolic content of Crocus leaves was 5.44 ± 0.01 mg GAE/g, and the total flavonoid content was 2.63 ± 0.05 mg RE/g, respectively. The main bioactive compounds in the leaves, such as polyphenols, flavonoids by HPLC and carboxylic acids, and amino acids, were also identified by GC-MS. HPLC analyses revealed mangiferin as a dominant constituent (1.26 ± 0.02 mg/g). C. sativus contains seven essential amino acids (ILE, LEU, LYS, MET, PHE, THR, TRP, VAL) in high concentration. Among them, isoleucine (7965 µg/g) was the dominant compound. In addition, the K and Ca concentrations in the leaves were significant (p < 0.05). The chemical composition revealed α-linolenic acid (22,490 µg/g) and linoelaidic acid (9880 µg/g) to be major constituents among all the acids found in the Crocus leaves. The extracts of C. sativus leaves showed the highest inhibitory activity for Gram-positive (B. subtilis and S. aureus) bacteria in the in vitro assay. The current results identify and underline the potential of natural products from C. sativus leaves that can add value to saffron production.

show abstract

Section: Amino Acids and Carboxylic Acids Assaysmentioning

confidence: 99%

Section: Gc-ms Analysis Of Amino Acids and Short-chained And Long-chain Carboxylic Acidsmentioning

confidence: 99%

Characterization of Phytochemical Components of Crocus sativus Leaves: A New Attractive By-Product

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, the amino acid composition of the plant extracts was shown to determine the activity against influenza A virus neuraminidase [ 70 , 71 ]. In our previous investigation, we studied the composition of amino acids in the water extract of C. sativus stigmas and found high content of amino acids including tyrosine (326.6 μg/g), methionine (84 μg/g), and alanine (60 μg/g) [ 72 ]. The stigmas extract was inactive in the other bioactivity tests (Table 4 ), including the anti-allergic (degranulation assay, 100 μg/mL), anti-viral (influenza H1N1 and enterovirus D68, 50 μg/mL), anti-inflammatory (respiratory burst and degranulation, 10 μg/mL), NRF2 expression in normal and cancer cell line (100 μg/mL), and lipid droplets assay (100 μg/mL).…”

Section: Resultsmentioning

confidence: 99%

Bio-guided bioactive profiling and HPLC-DAD fingerprinting of Ukrainian saffron (Crocus sativus stigmas): moving from correlation toward causation

Мykhailenko

Petrikaitė

Korinek

et al. 2021

BMC Complement Med Ther

Self Cite

View full text Add to dashboard Cite

Background Saffron or stigmas of Crocus sativus L. is one of the most valuable food products with interesting health-promoting properties. C. sativus has been widely used as a coloring and flavoring agent. Stigmas secondary metabolites showed potent cytotoxic effects in previous reports. Methods The present study investigated the chemical composition and the cytotoxic effect of Ukrainian saffron crude extracts and individual compounds against melanoma IGR39, triple-negative breast cancer MDA-MB-231, and glioblastoma U-87 cell lines in vitro using MTT assay. Several bioactivity in vitro assays were performed. The chemical profile of the water and hydroethanolic (70%, v/v) crude extracts of saffron stigmas was elucidated by HPLC-DAD analysis. Results Seven compounds were identified including crocin, picrocrocin, safranal, rutin, apigenin, caffeic acid, ferulic acid. Crocin, picrocrocin, safranal, rutin, and apigenin were the major active constituents of Ukrainian C. sativus stigmas. The hydroethanolic extract significantly reduced the viability of MDA-MB-231 and IGR39 cells and the effect was more potent in comparison with the water extract. However, the water extract was almost 5.6 times more active against the U-87 cell line (EC50 of the water extract against U-87 was 0.15 ± 0.02 mg/mL, and EC50 of the hydroethanolic extract was 0.83 ± 0.03 mg/mL). The pure compounds, apigenin, and caffeic acid also showed high cytotoxic activity against breast cancer, melanoma, and glioblastoma cell lines. The screening of the biological activities of stigmas water extract (up to 100 μg/mL) including anti-allergic, anti-virus, anti-neuraminidase, and anti-inflammatory effects revealed its inhibitory activity against neuraminidase enzyme by 41%. Conclusions The presented results revealed the qualitative and quantitative chemical composition and biological activity of Crocus sativus stigmas from Ukraine as a source of natural anticancer and neuraminidase inhibitory agents. The results of the extracts’ bioactivity suggested future potential applications of saffron as a natural remedy against several cancers.

show abstract

“…The standard solutions preparation was carried out according to Mykhailenko et al [ 21 ] with some modifications. One milligram of each reference standard and internal standard were diluted using deionized water, acetone, or ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…Quantification of 12 targeted metabolites was carried out according to the methods described in Mykhailenko et al [ 21 ], Murugesu et al [ 24 ], and Shi et al [ 25 ] with some modifications. The limit of quantification (LOQ), the limit of detection (LOD), linearity, precision, and trueness were determined using reference standards.…”

Section: Methodsmentioning

confidence: 99%

Muscle and Serum Metabolomics for Different Chicken Breeds under Commercial Conditions by GC–MS

Tan

Selamat

Jambari

et al. 2021

Foods

View full text Add to dashboard Cite

Globally, village chicken is popular and is known as a premium meat with a higher price. Food fraud can occur by selling other chicken breeds at a premium price in local markets. This study aimed to distinguish local village chicken from other chicken breeds available in the market, namely, colored broiler (Hubbard), broiler (Cobb), and spent laying hen (Dekalb) in pectoralis major and serum under commercial conditions using an untargeted metabolomics approach. Both pectoralis major and serum were analyzed using gas chromatography–mass spectrometry (GC–MS). The principal component analysis (PCA) results distinguished four different chicken breeds into three main groups for pectoralis major and serum. A total of 30 and 40 characteristic metabolites were identified for pectoralis major and serum, respectively. The four chicken breeds were characterized by the abundance of metabolites such as amino acids (L−glutamic acid, L−threonine, L−serine, L−leucine), organic acids (L−lactic acid, succinic acid, 3−hydroxybutyric acid), sugars (D−allose, D−glucose), sugar alcohols (myo−inositol), and fatty acids (linoleic acid). Our results suggest that an untargeted metabolomics approach using GC–MS and PCA could discriminate chicken breeds for pectoralis major and serum under commercial conditions. In this study, village chicken could only be distinguished from colored broiler (Hubbard) by serum samples.

show abstract

Comparative Investigation of Amino Acids Content in the Dry Extracts of Juno bucharica, Gladiolus Hybrid Zefir, Iris Hungarica, Iris Variegata and Crocus Sativus Raw Materials of Ukrainian Flora

Cited by 17 publications

References 54 publications

Characterization of Phytochemical Components of Crocus sativus Leaves: A New Attractive By-Product

Characterization of Phytochemical Components of Crocus sativus Leaves: A New Attractive By-Product

Bio-guided bioactive profiling and HPLC-DAD fingerprinting of Ukrainian saffron (Crocus sativus stigmas): moving from correlation toward causation

Muscle and Serum Metabolomics for Different Chicken Breeds under Commercial Conditions by GC–MS

Contact Info

Product

Resources

About