Bio-based products control black rot (Xanthomonas campestris pv. campestris) and increase the nutraceutical and antioxidant components in kale

Abstract

Black rot of crucifers, (Xanthomonas campestris pv. campestris) is the principal yield-limiting and destructive pathogen of cruciferous crop worldwide. In order to validate a bio-based control alternative for this disease, whey, lime sulfur, biofertilizer, Bordeaux mixture or raw milk were applied to kale (Brassica oleracea var. acephala) plants. The disease control was achieved by most of the tested products. Milk-based products (raw milk and whey) and biofertilizer reduced the severity by 44 and 56% in the field. Antioxidants, crude fibber, crude protein and lipid contents and kale yield were verified in the five treatments on the leaves with and without X. campestris pv. campestris inoculation. In the absence of the pathogen (non-inoculated), lime sulfur and Bordeaux mixture improved plant nutritional value compared to organic treatments, nevertheless milk-based products and biofertilizer improved the evaluated variables more than the control. However, on leaves inoculated with X. campestris pv. campestris raw milk increased antioxidant activity, crude protein and fiber contents, whereas biofertilizer increased kale yield, lipid and antioxidant contents. Milk-based products and biofertilizer were further evaluated in greenhouse trials to determinate the activity of defense-related enzymes and lignin content. Biofertilizer treatment resulted in increased phenylalanine ammonia lyase, catalase, peroxidase activities and lignin content. Hence, the application of milk-based products and biofertilizer are promising to control black rot of crucifers and also improves food quality by boosting nutritional values and antioxidant activity.

The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm

“…For plant-associated bacteria, the formation of biofilms has evolved as an adaptive strategy to successfully achieve host colonization and as a key strategy for pathogenesis [31]. Biofilm development contributes to the virulence of phytopathogenic bacteria through various mechanisms, including the blockage of xylem vessels [32].…”

Effect of Beta-Aminobutyric Acid on Activity of Peroxidase and Polyphenol Oxidase Enzymes in Cucumber Infected With Nematode Meloidogyne Javanica

“…[30]. Also it was found that changes of activity of the peroxidase enzyme showed the biochemical changes of plant and some of resistance reactions [22,31]. Reuveni and Bothma (1985) showed that increase of resistance of tomato plants inoculated with acibenzolar-S-methyl against bacterial canker of Clavibacter michiganensis ssp.…”

The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.)

“…The optimal growth temperature for X. campestris pv. campestris varies between 25-30 • C and spreading of the pathogen is strongly facilitated by humid and wet conditions (e.g., heavy rains, overhead irrigation) [4,41,42]. During summer months (June, July, and August) of 2017 in Lednice, 58% of the days reached maximum daily temperatures above 28 • C and average relative humidity from May to September was low (38%).…”