Bio-Aerosol Fluorescence

Pan, Yong‐Le; Eversole, Jay D.; Kaye, Paul H.; Foot, Virginia; Pinnick, Ronald G.; Hill, Steven C.; Mayo, Michael W.; Bottiger, Jerold R.; Huston, Alan L.; Sivaprakasam, Vasanthi; Chang, Richard K.

doi:10.1007/978-1-4020-5502-7_4

Cited by 13 publications

(7 citation statements)

References 86 publications

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“…There is always a tradeoff between the discrimination ability and negative false alarm rate, or the sensitivity (should be less confusing by using a detection threshold) and the probability of detection [67,70]. In order to reduce the positive false alarm rate, a system generally has to set at a high detection threshold, at the same time that will Fig.…”

Section: Algorithms For Discriminating Particular Bioaerosol From Atmmentioning

confidence: 98%

“…Such systems have also been used to study the fluorescence of bioaerosols; and other fluorescent particles in the laboratory (e.g., [29,32,10,97]), and to make continuous reagentless fluorescence measurements of atmospheric aerosols on several continents (e.g., [78,79,65,68,71,72,34,24,25,26,101,100,80,104]). Pan et al [67] summarized how to select the illuminating light source (laser, light-emitting diode (LED), or flash lamp); choose the most suitable detector (charge-coupled device (CCD), intensified CCD (ICCD), linear arrays of avalanche photodiodes, or multianode photomultiplier tube (PMTs)); and generate the test bioaerosol. They also reviewed the instrumentation of a few well-known detection systems.…”

Section: Fluorescent Molecules and Their Excitation-emission Matrix (mentioning

confidence: 99%

“…easily and clearly is always important. The spectral analysis algorithms used in the existing instruments can be grouped into three methodologies [67]. (1) Specific particle is determined by its signal location in a two-or threedimensional space where the coordinates are the fluorescence intensity, ratio of fluorescence intensity from UV and visible bands, or the size and shape information from elastic scattering of the detected aerosol particles, generally called scatter-plot.…”

Section: Algorithms For Discriminating Particular Bioaerosol From Atmmentioning

confidence: 99%

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Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Pan

2015

Journal of Quantitative Spectroscopy and Radiative Transfer

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117

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a b s t r a c tThis paper offers a brief review on the detection and characterization of biological and other organic-carbon (OC) aerosol particles in atmosphere using laser-induced-fluorescence (LIF) signatures. It focuses on single individual particles or aggregates in the micron and supermicron size range when they are successively drawn through the interrogation volume of a point detection system. Related technologies for these systems that have been developed in last two decades are also discussed. These results should provide a complementary view for studying atmospheric aerosol particles, particularly bioaerosol and OC aerosol particles from other analytical technologies.Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

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Section: Algorithms For Discriminating Particular Bioaerosol From Atmmentioning

confidence: 98%

Section: Fluorescent Molecules and Their Excitation-emission Matrix (mentioning

confidence: 99%

Section: Algorithms For Discriminating Particular Bioaerosol From Atmmentioning

confidence: 99%

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Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Pan

2015

Journal of Quantitative Spectroscopy and Radiative Transfer

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117

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“…Some of the figures shown here were also shown in Optics of Biological Particles (42), but were so reduced in size in that document that it was difficult to read them.…”

Section: Acknowledgmentsmentioning

confidence: 99%

Fluorescence of Bacteria, Pollens, and Naturally Occurring Airborne Particles: Excitation/Emission Spectra

Hill¹,

Mayo²,

Chang³

2009

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Approved for public release; distribution unlimited. FOR OFFICIAL USE ONLYii REPORT DOCUMENTATION PAGE Form Approved OMB No. 0704-0188Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number.

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“…LIF introduces advantages such as short pulse excitation, wavelength tunability, and narrow bandwidth excitation. This technique is of great importance for environmental monitoring of bioaerosols, due to capabilities for in situ analysis and remote sensing [3]. LIF technique has been applied for imaging and tracking a single molecule or particle in a biological cell [4,5].…”

Section: Introductionmentioning

confidence: 99%

Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules

Terzic

Marinković

Šević

et al. 2008

Facta Univ Phys Chem Technol

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Our developments of the time-resolved laser-induced fluorescence (TR-LIF) detection system for biomolecules are presented. This system is based on the tunable (320 nm to 475 nm) Nd:YAG laser pulses used to excite various biomolecules. The detection part is the Streak System for Fluorescence Lifetime Spectroscopy (Hamamatsu, Japan). The system consists of a C4334-01 streakscope, as a detector, DG 535 digital pulse/delay generator, C5094-S Spectrograph and HPD-TA System, as a temporal analyzer. The TR-LIF spectrometer is designed primarily to study the temperature and pressure effects on fluorescence behavior of biomolecules upon excitation with a single nanosecond pulse. The design of this system has capability to combine laser-induced breakdown (LIB) with fluorescence, as well to study optodynamic behavior of fluorescence biomolecules

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Bio-Aerosol Fluorescence

Cited by 13 publications

References 86 publications

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Detection and characterization of biological and other organic-carbon aerosol particles in atmosphere using fluorescence

Fluorescence of Bacteria, Pollens, and Naturally Occurring Airborne Particles: Excitation/Emission Spectra

Development of time-resolved laser-induced fluorescence spectroscopic technique for the analysis of biomolecules

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