Binuclear Cu(II) Schiff base complexes as prescursors for the synthesis of CuO nanoparticles: anticancer activity against MCF-7 cell line

Abstract

Homobinuclear Cu(II) complexes 1 and 2 were synthesized and characterized by elemental analysis, FTIR, 1 H NMR, Mass, UV-vis, magnetic moment, ESR and TGA studies. The synthesized complexes 1 and 2 were used as precursors for the synthesis of CuO Nps by calcination method. CuO Nps were characterized by FTIR, XRD, scanning electron microscopy (SEM), and EDX. XRD peaks clearly indicated the monoclinic structure of CuO. CuO nanoparticles were synthesized with average particle size 3-4 and 5-10 nm. The morphologies and size of the obtained products were further investigated by SEM. Presence of essential constituents of CuO Nps was investigated by EDX studies. The Anticancer activity of the precusors 1&2 and its nanoparticles have been analyzed against the MCF-7 cell line by MTT assay. The Precusor 1 with IC 50 value 12.66 μM was found to have high anticancer activity than other compounds. Synopsis:• Binuclear Cu(II) Schiff base complexes 1 & 2 were synthesized from the ligands L1 and L2, respectively. • Binuclear Cu(II) complexes were successfully used as a precursor for the synthesis of CuO Nps. • The ligands, precusors, and the CuO Nps were analyzed for their anticancer activity against the MCF-7 cell line. • Precursor 1 synthesized from the ligand L1 shows high activity than other compounds due to the presence of the heterocyclic moiety.CONTACT rajavel rangappan drrajavel@periyaruniversity.ac.in Downloaded by [McMaster University] at 23:21 23 June 2016 2 M. MOORTHy AND R. RANGAPPANBased on the above facts, herein we have reported the synthesis of CuO nanoparticles from the binuclear Schiff base copper complexes by calcination method and their characterization by various physicochemical methods. The precursors and its nanoparticles were analyzed for their anticancer activity against the MCF-7 cell line by MTT assay. Materials and methodsAll chemicals were of analytical reagent grade and were used as received. The elemental analysis was performed on Carlo-Erba 1106 model instrument at CDRI, Lucknow. The molar conductivity measurements were carried out using an Elico-CM Conductivity Bridge with 10 −3 M DMSO as solvent. FTIR spectra were recorded as KBr pellet using a Thermo Nicolet, Avatar 370 model FT-IR spectrophotometer ranging between 4000-400 cm −1 . Electronic absorption spectra were recorded on a Perkin Elmer Lambda 25 UV-vis spectrometer between 200-800 nm using DMSO solvent. The magnetic properties of the complexes were studied via a Gouy balance. 1 HNMR spectra for the ligand were recorded on a Bruker Avance III, 400-MHz NMR spectrometer using DMSO-d 6 solvent and TMS as internal standard. The EPR spectrum was recorded on a Bruker spectrometer operating in the X-band and using 100-kHz magnetic field modulation. The thermogravimetric analysis of the complexes were recorded on a Perkin Elmer STA 6000 thermal analysis system within the temperature range 40-740 °C. The SEM/EDS micrographs were recorded on a JEOL Model JSM -6390LV microscope. 2.