Binding studies of hydroxylated Multi-Walled Carbon Nanotubes to hemoglobin, gamma globulin and transferrin

Sekar, Gajalakshmi; Kandiyil, Shirona Thazae; Sivakumar, A.; Mukherjee, Amitava; Chandrasekaran, Natarajan

doi:10.1016/j.jphotobiol.2015.09.023

Cited by 26 publications

(14 citation statements)

References 49 publications

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“…The structure or environment changes of HSA, HHb, and Cyt c on their interactions with nano-selenium were deliberated by evaluating the inherent fluorescence of them. Most protein molecules have the residues of tryptophan, Trp-214 of HSA [ 57 ], three Trp units (α-Trp 14, ß-Trp 15 and ß-Trp 37) in each α and ß chain of HHb, and Trp-59 of Cyt c [ 58 ], which led to the fluorescence of them at approximately 346 nm [ 59 ]. The inherent fluorescence of HSA, HHb, and Cyt c in the presence of various concentrations of nano-selenium at three different temperatures under physiological conditions (pH = 7.4) was noted ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

Shahabadi

Zendehcheshm

Khademi

2021

Biotechnology Reports

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Section: Resultsmentioning

confidence: 99%

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

Shahabadi

Zendehcheshm

Khademi

2021

Biotechnology Reports

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“…It has been reported that functionalized carbon nanotubes induced substantial conformational changes in HHb structure, regardless of single-wall or multi-wall structures. 40,41 Moreover, it was shown that nanodiamonds can drive secondary structural changes in HHb structure in a concentration-dependent manner. 20 However, this study demonstrated that CNPs do not perturb the secondary structure of Hb.…”

Section: Figure 12mentioning

confidence: 99%

Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro

Eskandari¹,

Babadaei²,

Nikpur³

et al. 2018

IJN

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IntroductionThe application of nanoparticles (NPs) in medicine and biology has received great interest due to their novel features. However, their adverse effects on the biological system are not well understood.Materials and methodsThis study aims to evaluate the effect of cerium oxide nanoparticles (CNPs) on conformational changes of human hemoglobin (HHb) and lymphocytes by different spectroscopic (intrinsic and synchronous fluorescence spectroscopy and far and near circular dichroism [CD] spectroscopy), docking and cellular (MTT and flow cytometry) investigations.Results and discussionTransmission electron microscopy (TEM) showed that CNP diameter is ~30 nm. The infrared spectrum demonstrated a strong band around 783 cm−1 corresponding to the CNP stretching bond. Fluorescence data revealed that the CNP is able to quench the intrinsic fluorescence of HHb through both dynamic and static quenching mechanisms. The binding constant (Kb), number of binding sites (n), and thermodynamic parameters over three different temperatures indicated that hydrophobic interactions might play a considerable role in the interaction of CNPs with HHb. Synchronous fluorescence spectroscopy indicated that microenvironmental changes around Trp and Tyr residues remain almost unchanged. CD studies displayed that the regular secondary structure of HHb had no significant changes; however, the quaternary structure of protein is subjected to marginal structural changes. Docking studies showed the larger CNP cluster is more oriented toward experimental data, compared with smaller counterparts. Cellular assays revealed that CNP, at high concentrations (>50 µg/mL), initiated an antiproliferative response through apoptosis induction on lymphocytes.ConclusionThe findings may exhibit that, although CNPs did not significantly perturb the native conformation of HHb, they can stimulate some cellular adverse effects at high concentrations that may limit the medicinal and biological application of CNPs. In other words, CNP application in biological systems should be done at low concentrations.

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“…40 The interaction of Hb, gamma globulin, and transferrin with hydroxyl group-modified multi-walled carbon nanotubes was explored by several spectroscopic methods. 41 Probable changes around the aromatic microenvironment of these proteins were shown. Also, possible alterations toward their secondary structure after interactions with modified multi-walled carbon nanotubes were revealed.…”

Section: Discussionmentioning

confidence: 99%

“…This investigation provided useful information regarding the biosafety profile of functionalized multi-walled carbon nanotubes for their in vivo biomedical implementations. 41 The interaction of nanodiamond and silicon dioxide NPs with Hb also showed secondary and tertiary structural changes of Hb. 42,43 It has been shown that fullerene NPs synthesized by different methods induce various impacts on human serum albumin and bovine serum albumin conformations.…”

Section: Discussionmentioning

confidence: 99%

Biophysical, bioinformatical, cellular, and molecular investigations on the effects of graphene oxide nanosheets on the hemoglobin structure and lymphocyte cell cytotoxicity

Babadaei¹,

Moghaddam²,

Solhvand³

et al. 2018

IJN

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BackgroundImplementations of nanoparticles have been receiving great interest in medicine and technology due to their unique characteristics. However, their toxic impacts on the biological system are not well explored.AimThis study aims to investigate the influence of fabricated nano graphene oxide (NGO) sheets on the secondary and quaternary structural alterations of human hemoglobin (Hb) and cytotoxicity against lymphocyte cells.Materials and methodsDifferent spectroscopic methods, such as extrinsic and synchronous fluorescence spectroscopy and far circular dichroism (CD) spectroscopy, molecular docking investigation, cellular assays (trypan blue exclusion, cellular uptake, ROS, cell cycle, and apoptosis), and molecular assay (fold changes in anti/proapoptotic genes [B-cell lymphoma-2 {BCL2}/BAX] expression levels) were used in this study.ResultsTransmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and zeta potential investigations revealed the nano-sized nature of NGOs with good colloidal stability. Extrinsic fluorescence spectroscopy by using 8-anilinonaphthalene-1 -sulfonic acid and synchronous fluorescence spectroscopy showed that NGOs can unfold the quaternary structure of Hb in the vicinity of Tyr residues. The CD investigation demonstrated that the α-helicity of Hb experienced substantial alteration upon interaction with increasing concentrations of NGOs. The molecular docking study showed that NGOs interacted with polar residues of Hb. Cellular and molecular assays revealed that NGOs lead to ROS formation, cell cycle arrest, and apoptosis through the BAX and BCL2 pathway.ConclusionThese data reveal that NGOs can induce some protein structural changes and stimulate cytotoxicity against normal cell targets. Therefore, their applications in healthy systems should be limited.

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Binding studies of hydroxylated Multi-Walled Carbon Nanotubes to hemoglobin, gamma globulin and transferrin

Cited by 26 publications

References 49 publications

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

Selenium nanoparticles: Synthesis, in-vitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HSA, HHb and Cyt c serum proteins

Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro

Biophysical, bioinformatical, cellular, and molecular investigations on the effects of graphene oxide nanosheets on the hemoglobin structure and lymphocyte cell cytotoxicity

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