Binding of type 1-piliated Escherichia coli to vaginal mucus

Venegas, Mario F.; Navas, Elena L.; Gaffney, Robert A.; Anderson, Byron; Schaeffer, Anthony J.

doi:10.1128/iai.63.2.416-422.1995

Cited by 36 publications

(14 citation statements)

References 38 publications

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“…The sugar structures presumably consist of short oligomannose chains bound via N-glycosidic linkages to the glycoproteins (216). The relevance of these pili to bacterial virulence is thought to arise mainly from binding of the bacteria to mucus or to epithelial cells of the urogenital, respiratory, and intestinal tracts (16,162,244). Their role in the pathogenesis of UTI was clarified mostly in studies on E. coli but has also been described for K. pneumoniae in animal models (77,78,134).…”

Section: Pili (Fimbriae)mentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

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SUMMARY Bacteria belonging to the genus Klebsiella frequently cause human nosocomial infections. In particular, the medically most important Klebsiella species, Klebsiella pneumoniae, accounts for a significant proportion of hospital-acquired urinary tract infections, pneumonia, septicemias, and soft tissue infections. The principal pathogenic reservoirs for transmission of Klebsiella are the gastrointestinal tract and the hands of hospital personnel. Because of their ability to spread rapidly in the hospital environment, these bacteria tend to cause nosocomial outbreaks. Hospital outbreaks of multidrug-resistant Klebsiella spp., especially those in neonatal wards, are often caused by new types of strains, the so-called extended-spectrum-β-lactamase (ESBL) producers. The incidence of ESBL-producing strains among clinical Klebsiella isolates has been steadily increasing over the past years. The resulting limitations on the therapeutic options demand new measures for the management of Klebsiella hospital infections. While the different typing methods are useful epidemiological tools for infection control, recent findings about Klebsiella virulence factors have provided new insights into the pathogenic strategies of these bacteria. Klebsiella pathogenicity factors such as capsules or lipopolysaccharides are presently considered to be promising candidates for vaccination efforts that may serve as immunological infection control measures.

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Section: Pili (Fimbriae)mentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

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“…These factors include structural features such as fimbriae or pili for adherence, flagella for motility and chemical adhesion [3]. The type-1 pili of uropathogenic E. coli are known to be associated with increased severity of UTI by binding to mannose containing glycoprotein receptors on facet cells lining the bladder or vaginal epithelial cells [4]. Other virulence factors which confer the ability to fecal E. coli to colonize the vaginal mucosa and cause symptomatic UTI have also been identified [3].…”

Section: Introductionmentioning

confidence: 99%

Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection

Suhani¹,

Purkaystha²,

Begum³

et al. 2017

Clin Microbiol

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Background: Escherichia coli causes urinary tract infection (UTI), one of the most frequent bacterial infections in humans, shows resistance to various commonly prescribed antibiotics. This is termed as multi drug resistance, which is increasing among E. coli. The multi-drug resistant E. coli isolates harbor multiple plasmids that might be responsible for antibiotic resistance. The resistance can be transferred among bacteria through horizontal transfer of the genetic materials. Objectives: We undertook this study 1) to find a link between antibiotic resistance and plasmid of E. coli from UTI patients of Sylhet region of Bangladesh 2) to analyze the spread of the plasmid mediated antibiotic resistant gene between E. coli isolates. Methods: Conjugation experiment was carried out in Luria Broth with E. coli DH5α as recipient. For transformation, competent cells were prepared using E. coli DH5α. Plasmid isolation was done by mini alkaline lysis method and plasmid was extracted from agarose gel and transferred to the competent cell. Results: After conjugation, the donor E. coli isolate that showed resistance to amoxicillin (AMX), ciprofloxacin (CIP) and ceftriaxone (CTR) tranferred its AMX and CIP resistance to the recipient E. coli DH5α, that was previously sensitive to all antibiotics. After transformation, the recipient E. coli DH5α became resistant to CIP and AMX, while the donor showed resistance to gentamycin (CN), ceftriaxone (CTR), amoxicillin (AMX), ciprofloxacin (CIP), cefixime CFM, and cotrimoxazole (COT). Plasmid extracted from the transformant revealed that, three genes (3 kb, 5 kb and 20 kb) have been transferred from the donor to recipient. Conclusion: Plasmids responsible for amoxicillin and ciprofloxacin were transferred.

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“…Previous work from our laboratory demonstrated that every vaginal fluid sample tested bound type 1-piliated bacteria and this binding was inhibited by mannose (37). In another study, it was shown that vaginal fluid influenced the adherence of type 1-piliated E. coli to epithelial cells in vitro (7).…”

mentioning

confidence: 82%

“…Some of the postmenopausal women were receiving hormone replacement therapy. The fluids were collected and processed as described earlier (7,37). Briefly, the specimens were collected by gently scraping the vaginal mucosa with sterile Dacaron-tipped applicators (Hardwood Products Company, Guilford, Mass.)…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

Roles of Glycoproteins and Oligosaccharides Found in Human Vaginal Fluid in Bacterial Adherence

Rajan¹,

Cao²,

Anderson

et al. 1999

Infect Immun

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Adherence of type 1-piliated Escherichia coli to carbohydrate structures of vaginal mucosa plays a major role in the pathogenesis of ascending urinary tract infections in women. Colonization of the vaginal introitus is influenced by interactions between pathogens, vaginal fluid, and vaginal epithelium. In this study, the type and amount of carbohydrates and glycoproteins present in vaginal fluid were determined. Free and protein-bound oligosaccharides in vaginal fluid specimens were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE) and high-pressure liquid chromatography (HPLC). Two-dimensional electrophoretic separations of vaginal fluid glycoproteins were performed together with bacterial overlay assays. The results of FACE showed that the majority of the oligosaccharides are in the free state and the bound oligosaccharides are undetectable. HPLC analysis of free sugars revealed glucose as the major sugar (3.3 ؎ 0.3 mM), and the concentrations of mannose and glucosamine were 0.065 ؎ 0.04 and 0.02 ؎ 0.001 mM, respectively. Radiolabeled E. coli bound three vaginal fluid glycoproteins with the following molecular masses and pIs: 82 kDa and pI 5.5, 55 kDa and pI 4.5, and 55 kDa and pI 6.5. The binding was inhibited by mannose and by deglycosylation of the proteins prior to the overlay assay. One of these putative receptors was identified to be the heavy chain of secretory IgA (S-IgA). These data suggest that the free mannose in the fluid is less than that required to affect E. coli-epithelial cell binding interactions and that S-IgA may bind E. coli in the vaginal introitus.

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Binding of type 1-piliated Escherichia coli to vaginal mucus

Cited by 36 publications

References 38 publications

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection

Roles of Glycoproteins and Oligosaccharides Found in Human Vaginal Fluid in Bacterial Adherence

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