Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection

Suhani, Sabrina; Purkaystha, Auditi; Begum, Musammat Kulsuma; Islam, Md Jahidul; Azad, Abul Kalam

doi:10.4172/2327-5073.1000284

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…The only isolate in the present investigation with 100% resistance against the tested antibiotics (EC91) was seen to carry multiple bands with different sizes and this could be the contributory factor in the exhibited resistance in this isolate. Also studies have shown that penicillin resistant E. coli harboured plasmids that are within the range of 30 kb and above [33], such as is the case of the E. coli isolates in the present investigation. Thus, E. coli showing MDR have been reported to carry multiple plasmids of different sizes and this has been the case of those encountered in this study.…”

Section: Discussionsupporting

confidence: 66%

“…With high resistance being attributed to plasmids having molecular weights of 23-26 kb, the resultant high resistance as seen in this study could be due to the 30kb and above of the molecular weight of the plasmids as well as the presence of multiple sizes amongst the isolates. However, it is known that E. coli isolates carry multiple plasmids which could be the reason why they are resistant to antibiotics [33]. The only isolate in the present investigation with 100% resistance against the tested antibiotics (EC91) was seen to carry multiple bands with different sizes and this could be the contributory factor in the exhibited resistance in this isolate.…”

Section: Discussionmentioning

confidence: 56%

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Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Badger-Emeka¹,

Ineta²,

Emeka³

et al. 2018

biomedicalresearch

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Background/Aims: The role of mobile genetic elements such as plasmids in the spread of multiple antibiotic resistance has continued to receive attention by researchers. Due to the diverse nature of Escherichia coli strains characteristically harbouring large proportion of genomic plasmids, the aim of the present study is to evaluate the antimicrobial susceptibility and plasmid profiles of clinical isolates of E. coli in this south eastern region of Saudi Arabia. Methods: Bacterial isolation, antimicrobial susceptibility test were carried out using basic microbiological techniques. Dendrogram of clustering in relationship to antibiotic susceptibility of the isolates was carried out using PAST. Extracted plasmid DNA was visualised using the ultraviolet light illuminator and photo documentation system. Sizes of Plasmid were determined using standard DNA molecular weight markers at 1kb ladder. Plasmid curing was carried out using Sodium Dodecyl Sulphate (SDS). Results and conclusion: Antibiotic resistance ranged between 85.71%-100% for the cephalosporins, 64.29% to 92.9% resistance for penicillins, including Aztreonam, a last line drug. Results indicate that 70% of the isolates did not show any plasmid presence. However, 30% of the isolates contained plasmids with molecular weights of 30kb and above. Dendrogram of clustering in relationship to antibiotic susceptibility showed isolates were in 3 clades pre-plasmid curing and 6 clades post-plasmid curing. One isolate (EC36) was cured of its resistance to Penicillins, while isolates EC67, EC77 and EC87 initially sensitive to ciprofloxacin, became resistant to the antibiotic. The findings suggest a preponderance of chromosomal mediated resistant E. coli. The few plasmid containing resistant bacteria, were resistant to curing as they displayed continued antibiotic resistance after curing. This therefore confirms the growing presence of multiple genomic plasmid harbouring E. coli.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 56%

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Badger-Emeka¹,

Ineta²,

Emeka³

et al. 2018

biomedicalresearch

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“…About 90% of the E. coli isolates causing UTI in the North-East region of Bangladesh were reported as MDR, and the multidrug resistance in E. coli might be either plasmid or chromosomal DNA mediated or the both. 2,13 In this study, the efflux pump system has been discussed as one of the reasons for multidrug resistance in the MDR E. coli isolates causing UTIs and omeprazole may be an efflux pump inhibitor.…”

Section: Resultsmentioning

confidence: 99%

“…Susceptible bacteria can acquire resistance to an antimicrobial agent through mutation, degradation, or changing the structure of antibiotic, efflux pump, and conjugation and/or transformation of the plasmid. 2,13,14 Efflux pump proteins, found in both Gram-positive and Gram-negative bacteria as well as in eukaryotic microorganisms, 15 are transporter proteins involved in the extrusion of toxic substrates from within cells into the external environment. The impact of efflux mechanisms on AMR is large, and antibiotics can act as inducers and repressors of the expression of some efflux pump proteins.…”

Section: Introductionmentioning

confidence: 99%

Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR from Omeprazole Responsive Multidrug-Resistant Escherichia coli Isolates Causing Urinary Tract Infections

et al. 2019

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Antimicrobial resistance poses a threat in the treatment of infectious diseases in Bangladesh as well as in the world. Multidrug-resistant (MDR) Enterobacteriaceae, the most common cause of one such infectious disease, urinary tract infection (UTI), has contributed to the escalating problem of selecting empiric antibiotics against UTIs. The aim of this study was to investigate the presence of the efflux pump in MDR Escherichia coli isolates from UTI in the North-East region of Bangladesh, to isolate and characterize the AcrAB-TolC efflux pump genes of these locally isolated strains and to do mutation analysis of the efflux pump repressor AcrR gene to understand the AcrAB-TolC efflux pump mechanism. In the presence of omeprazole, an efflux pump inhibitor, every MDR E. coli isolate showed increased susceptibility to at least 1 of the 7 antibiotics investigated, indicating that efflux pump might be involved in their antibiotic resistance. Omeprazole decreased the minimum inhibitory concentration of every antibiotics being investigated by 2- to 8-fold. DNA and the deduced amino acid sequences of the polymerase chain reaction (PCR) products analyzed by bioinformatics tools revealed that the chromosomal AcrAB-TolC and AcrR genes were present in all MDR and antibiotic-susceptible E. coli isolates. However, the deduced amino acid sequences of the amplification refractory mutation system (ARMS) PCR product of the AcrR gene revealed that the substitution of arginine to cysteine at position 45 of AcrR was observed only in the MDR E. coli whose antibiotic susceptibility increased in the presence of omeprazole. Data reported herein support the notion that the increased antibiotic susceptibility of the MDR E. coli isolates in the presence of omeprazole might be due to efflux pump(s) inhibition and the AcrAB-TolC efflux pump might be a contributor to antibiotic resistance when the mutation of arginine to cysteine occurs at position 45 of AcrR.

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“…The presence of three heavy molecular weight plasmids found in E. coli corroborates previous study which showed that E. coli isolates carry multiple plasmids which could be the reason for their resistant to antibiotics [21]. Bacteria antibiotics resistance patterns are sometimes associated with the presence of large plasmids and ability of plasmids for conjugation process [22].…”

Section: Discussionmentioning

confidence: 99%

Microbial Assessment of Bed Linens in Ekiti State University Students’ Hostels

Olowomofe

Oluyege

Ogunlade

et al. 2020

JAMB

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Bed linen is clearly recognized as a potential reservoir for microorganisms and could be a vector of disease transmission. The present study was aimed at isolating and characterizing bacteria and fungi from different kinds of bed linen of student in Ekiti State University hostels. Pour plate method was used for the enumeration of total bacteria count from the posterior and anterior end of the bed linen. The average bacteria count for the anterior and posterior end was 7.46 and 7.16 Log10 CFU/ml respectively. The most dominant microbial species were bacteria and these were mostly found in the environment and on human skin. The bacteria isolated were Staphylococcus aureus, Staphylococcus epidermidis, Proteus mirabilis, Enterobacter aerogenes, Bacillus cereus, Escherichia coli and Klebsiella aerogenes. Bacillus cereus had the highest frequency of occurrence (25%), followed by Staphylococcus aureus (15%), Escherichia coli (15%), Klebsiella aerogenes (15%), Staphylococcus epidermis (15%), Enterobacter aerogenes (10%) and Proteus mirabilis (5%). The fungi isolates were Aspergillus sulphureus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus and Fusarium spp. Antibiotics susceptibility test was carried out on the bacteria isolates with gram negative bacteria showing resistance to Cotrimoxazole and gram positive bacteria showing resistance to Amplicillin. Most of the bacteria isolates have multiple antibiotics resistance. The bacterial isolates were susceptible to Ciprofloxacin. Plasmid profiling was also done with Escherichia coli having three high molecular weight plasmids, Bacillus cereus and Enterobacter aerogenes do not possess plasmid. The identified species are suspected to be opportunistic pathogens for human, representing a risk for people with weakened immune system, especially in cases of super-infection.

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Plasmids for Amoxicillin and Ciprofloxacin Resistance in Escherichia coli Isolate Causing Urinary Tract Infection

Cited by 5 publications

References 30 publications

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Antibiotic susceptibility and plasmid profile of clinical isolates of Escherichia coli

Identification of AcrAB-TolC Efflux Pump Genes and Detection of Mutation in Efflux Repressor AcrR from Omeprazole Responsive Multidrug-Resistant Escherichia coli Isolates Causing Urinary Tract Infections

Microbial Assessment of Bed Linens in Ekiti State University Students’ Hostels

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