Binding of Taurocholate by Pectin in the Presence of Calcium Ions

Abstract

Binding of taurocholate by pectin was examined in vitro, using lowmethoxyl pectin and calcium lactate. Water in calcium pectate gel was partially separated from the gel by filtration through a glass-filter. Taurocholate bound by pectin was calculated after measurement of taurocholate in the filtrate by the UV absorption method. The amount of taurocholate bound by pectin increased linearly with increase in pectin concentration but independent of time. One gram pectin bound a maximum of 6.0 Pmole of taurocholate.

Effect of pH on the in vitro interactions between bile acids and pectin

“…In this instance, it is possible that Ca 2+ forms bridges between the acid groups of BA and the free COOH of pectin. In some studies such bridges of di-or trivalent cations are discussed [11,15,17]. There was no evidence from the present experiments for an enhancement of the interaction between BA and pectin in the presence of Ca 2+ .…”

Oral Acute and Subacute Toxicity Studies with Kalpaamruthaa, a Modified Indigenous Preparation, on Rats

“…45) Bile acid adsorption by dietary fiber in vitro has also been reported for many fiber types. 46) In addition polyphenols such as flavonoids and tannins have been shown to have numerous health protective benefits of which include lowering of blood lipids. Furthermore recent reports have suggested that several plant sterols reduce serum cholesterol by the inhibition of intestinal cholesterol absorption.…”