Binding of Streptavidin with Biotinylated Thermosensitive Nanospheres Based on Poly(N,N-diethylacrylamide-co-2-hydroxyethyl methacrylate)

Abstract

Thermosensitive polymer nanospheres based on N,N-diethylacrylamide and 2-hydroxyethyl methacrylate (HEMA) have been prepared, characterized, and conjugated with biotin. The thermosensitivity of poly(N,N-diethylacrylamide) was enhanced by the incorporation of HEMA up to about 40 mol %. Atomic force microscopic images show that these particles can be closely packed even without the surface charges as in the latex particles. Biotinylation reduces the thermosensitivity of the copolymer nanospheres. The biotinylated hydrogel nanospheres showed a reduction in size upon binding with streptavidin, indicating the formation of a less hydrophilic conjugate. No aggregation of the biotinylated particles due to the cross-linking effect of streptavidin was observed. This size change could be reversed by the addition of free biotin to the system. The interaction is specific, and no such changes were observed when streptavidin was replaced by bovine serum albumin.

Responsive properties of crystalline colloidal arrays formed by core-shell microspheres with pH and temperature sensitivities

“…1 Stimuli-responsive microgels have proven to be particularly interesting because of their ability to swell or shrink, depending on their environment 2,3 or their interaction with an analyte, such as the complexation with a biological molecule. [4][5][6] Crystalline colloidal arrays (CCAs) may be formed through the self-assembly of these particles, and the stimuli-responsiveness combined to their visible light diffraction make these structures interesting and useful. CCAs may be used as photonic gap materials 7,8 and biosensors, 9,10 mainly in the form of polymerized crystalline colloidal arrays, where the self-assembled hard spheres are fixed inside a crosslinked hydrogel matrix, which is responsive toward the external stimulus.…”

Pentapeptide‐modified poly(N,N‐diethylacrylamide) hydrogel scaffolds for tissue engineering

“…Additional aim was to investigate cytotoxicity of the hydrogels, penetration and proliferation of cells in the scaffolds and to determine optimal cell cultivation conditions using well‐characterized hESC lines, CCTL12, and CCTL14. PDEAAm has a volume phase transition temperature (VPTT) of 25–32°C, close to body temperature, a property that can be useful, for instance, in site‐specific controlled drug delivery systems9, 14 or biosensor applications 15. PDEAAm hydrogels swell in water at room temperature, but deswell and shrink at temperatures above the VPTT.…”

Thermoresponsive Synergistic Hydrogen Bonding Switched by Several Guest Units in a Water‐Soluble Polymer

“…The hydrophobic3 or hydrophilic4 comonomers were generally incorporated into polymer chains via statistic copolymerization to modify LCST. Thermoresponsive behavior could also be tuned by adjusting the composition of statistic copolymers,5 the sequence of block copolymers,6 or the gradient distribution of copolymers 7. Most recently, Zhu and coworkers8 reported the stepwise‐thermosensitivity of block copolymers based on two segments of random copolymers.…”