Binding of Soluble Immune Complexes to Fc Receptors on Human Neutrophils – Detection by Double-Coating Indirect Rosette Formation

An, Teisa

doi:10.1159/000232446

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Anti-ferritin (anti-Fer) and anti-sheep red blood cells were prepared in alalb4b4 rabbits as described before [4], F(ab'); fragments and 7S fractions of antisera were prepared as de scribed [7]. Apoferritin was prepared from crystallized Fer (Sigma, St. Louis, Mo.)…”

Section: Methodsmentioning

confidence: 99%

“…Binding of the ligand for FcR on human lymphocytes was detected by using a modified Coombs mixed antiglobulin reaction which we called DIRF [4,7,12]. In brief, human lymphocytes were coated at 0°C (ice bath) with the ligand for FcR; the coated lymphocytes formed rosettes (indirect rosettes) with sheep erythrocytes sensitized with a subhemagglutinating dose of F(ab')2 fragments of b4 rabbit anti-SRBC with interposing bridges of F(ab')2 anti-b4 at 4°C in FCS-PBS or FCS-PBS-R. Lymphocytes with four or more adhering indi cator cells were expressed as a percentage of total counted lympho cytes.…”

Section: Double-coaling Indirect Rosette Formation (Dirf)mentioning

confidence: 99%

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Are Fcγ Receptors Naturally Clustered on Normal Human B Lymphocytes?

An¹

1984

Int Arch Allergy Immunol

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The Fcγ receptor (FcR) on normal human B lymphocytes was studied by using electron-microscopic monovalent ligands made of one ferritin (Fer) and one rabbit IgG anti-Fer. The binding of ligands was ascertained with the modified Coombs mixed antiglobulin reaction. The receptors were found clustered at 0°C, which was not affected even by examining the coated cells without washing away excess of the ligand solution, or by cross-linking bound ligands with a second antibody. The clusters varied in length, measuring up to 220 nm. Most of them were less than 110 nm long, and were seen as spots. When lymphocytes were preincubated with both cytochalasin B and colchicine, the clustering of FcR was not affected. Our findings show that FcR on B lymphocytes are naturally aggregated, which is not primarily mediated with the cytoskeletons made of actin microfilaments and microtubules.

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Section: Methodsmentioning

confidence: 99%

Section: Double-coaling Indirect Rosette Formation (Dirf)mentioning

confidence: 99%

Are Fcγ Receptors Naturally Clustered on Normal Human B Lymphocytes?

An¹

1984

Int Arch Allergy Immunol

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Fc receptor—More answers, more questions

Fornůsek

Větvička

1984

Folia Microbiol

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Fc receptors, belonging to the most important surface structures of a number of cells participating in the immune processes, have been intensely studied during the past decade. The present review summarizes the contemporary knowledge of the specificity and heterogeneity of Fc receptors and of factors influencing their expression, and includes some views on their function. In addition, it mentions their relationship to other cell surface structures, expression of Fc receptors during ontogeny of the organism and in certain diseases. Finally, data concerning the isolation and biochemical characterization of the Fc receptor molecule are presented.

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Suppression of zymosan-induced chemiluminescence of whole blood by conjugates composed of drug, guinea pig skin extracts and sera from patients with suspicious drug eruptions.

Kato

Terada

Kuramoto³

et al. 1983

Tohoku J. Exp. Med.

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Conjugates composed of drug, guinea pig skin soluble fraction, and sera from 50 patients with various types of drug eruptions were prepared by the incubation of these mixtures for 60 min at 37°C . And the effects of conjugates on zymosan-induced chemiluminescence (ZI-CL) in whole blood were examined. The conjugates obtained from 32 cases significantly suppressed ZI-CL of whole blood. The mean value of peak intensities of CL in the presence of their conjugates showed 62% of those after the pretreatment of mixtures consisting of drug, soluble fraction and sera from healthy donors. In the systems of non-opsonized and opsonized zymosan-induced CL of leukocyte-erythrocyte sediment, similar inhibitory effects were obtained in both systems in the presence of conjugates, but the time course of CL response was slower in the system with non-opsonized zymosan. In other experiments with the use of immune complexes, which were formed by human albumin and antihuman albumin goat serum in vitro, soluble immune complexes suppressed ZI-Cl of whole blood. These results suggest that immune complexes may be formed during incubation of conjugates and block the receptors on the surfaces of granulocytes, resulting in the suppression of ZI-CL. chemiluminescence of whole blood ; granulocytes ; drug eruptions ; immune complexes

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Binding of Soluble Immune Complexes to Fc Receptors on Human Neutrophils – Detection by Double-Coating Indirect Rosette Formation

Cited by 3 publications

References 6 publications

Are Fcγ Receptors Naturally Clustered on Normal Human B Lymphocytes?

Are Fcγ Receptors Naturally Clustered on Normal Human B Lymphocytes?

Fc receptor—More answers, more questions

Suppression of zymosan-induced chemiluminescence of whole blood by conjugates composed of drug, guinea pig skin extracts and sera from patients with suspicious drug eruptions.

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