Binding of Ricinus communis I lectin to the muscle cell plasma membrane in diseased muscle

Capaldi, M.J.; Dünn, Michael J.; Sewry, Caroline A.; Dubowitz, Victor

doi:10.1016/0022-510x(84)90179-5

Cited by 15 publications

(3 citation statements)

References 10 publications

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“…There are several manuscripts with lectin binding cell membranes and cell components for example myofibrills (24,(29)(30)(31). The binding characteristic of lectins with varying sugar specificities were investigated by Dunn et al, (24).…”

Section: Discussionmentioning

confidence: 99%

Lectin Staining of Extensor Digitorum Longus Muscle Cell Membranes in Alloxan Diabetic Rats

Gül¹,

Cebesoy²,

Özsoy³

2017

Turk J Diab Obes

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Amaç: Alloksan diyabetin sıçanların Extensor Digitorum Longus (EDL) iskelet kasına etkisi lektin boyama tekniği ile ışık mikroskobunda incelendi. Gereç ve Yöntemler:İntravenöz yoldan alloksan enjekte edildikten 30 gün sonra, kontrol ve diyabetik grubu sıçanlardan kas örnekleri alındı. Kas örneklerinden kryostatlı mikrotom ile kesitleri alındı ve dört biyotinli lektin [Wheat Germ Agglutinin (WGA), Pea Nut Agglutinin (PNA), Concavalin A (ConA), Griffonia simplicifolia (GS)] ile boyandı. Lektinler, avidin-peroksidaz kompleksi ile tespit edildi. Lektinlerin hepsi kontrol ve diyabetik sıçan grubunun EDL iskelet kasına bağlandı. Bulgular: GS ve WGA lektinleri alloksan diyabetik sıçanların EDL iskelet kaslarını kuvvetli (+++) boyadı. Özellikle GS lektini sadece hücre zarına değil aynı zamanda kas fibrillerini kuvvetli boyadı. PNA lektini orta derecede (++) EDL kaslarını boyadı. Con A ise kontrol grubuna göre diyabetik kasları daha zayıf (+) boyadı. Elde ettiğimiz bulgulara göre alloksan-diyabet sıçanların EDL iskelet kaslarının hücre zarlarındaki glikoproteinlerin moleküler yapısını değiştirmiştir.Sonuç: Bu çalışma diyabetin hücre zarlarına hasar verici etkisini göstermek açısından diyabetle yapılan araştırmalara katkıda bulunacaktır. Anahtar Sözcükler: Extensor digitorum longus iskelet kası hücre zarı, Lektin boyama, Diabetes mellitus, Alloksan, Wistar sıçanABSTrAcT Aim: Effect of alloxan-diabet on Extensor digitorum longus (EDL) skeletal muscle of rats were observed by lectin staining techniques in light microscope. Material and Methods:After 30 days alloxan (dose of alloxan: 55 mg/kg) injected by intravenously, samples of muscles were obtained from control and diabetic rats. The muscle samples sections were cutted by cryostat microtome and stained by four biotinylated lectins [Wheat Germ Agglutinin (WGA), Pea Nut Agglutinin (PNA), Concavalin A (ConA), Griffonia simplicifolia I (GS)]. The lectins were fixed by avidin-peroxidase complex. All lectins were bounded to EDL muscle cell membranes of control and diabetic rats.results: GS and WGA lectins were strongly (+++) stained extensor digitorum muscle cell membranes of alloxan-diabetic rats. Not only cell membranes but also cytoplasmic miyofibrills of diabetic muscle cells were stained by GS lectin. PNA was moderate (++) stained diabetic muscle cell membranes. Con A was weakly (+) stained diabetic muscle cell membranes with respect to control cell membranes. According to our findings, alloxan-diabetes altered the molecular structure of glycoproteins in cell membranes of EDL skeletal muscles of rats. conclusion:We suggested that this study will contribute to diabetes research to show the damaging effects of diabetes on cell membranes.

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Section: Discussionmentioning

confidence: 99%

Lectin Staining of Extensor Digitorum Longus Muscle Cell Membranes in Alloxan Diabetic Rats

Gül¹,

Cebesoy²,

Özsoy³

2017

Turk J Diab Obes

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“…Some reports have described the application of lectin histochemistry to normal and pathological skeletal muscles at both the light microscopic (11,16,25,26) and electron microscopic levels (7,8,10,11). These previous studies and our present studies demonstrate that in normal muscle, PNA and RCA-I specific to 9-D-galactosyl residues, and Con A specific to a-D-mannosyl residues stained the surface of the muscle fibers, perimysial and endomysial connective tissue, and capillaries.…”

Section: Discussionmentioning

confidence: 99%

Lysosomal glycogen storage disease without acid maltase deficiency. A lectin-histochemical study of an unusual type of lysosomal glycogen storage disease.

Kashio

Usuki

Higuchi

et al. 1991

Acta Histochem. Cytochem

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“…The presence of MHC (HLA) class I antigens on the sarcolemma led to the appreciation of its importance in inflammatory myopathies. Mike Dunn's studies of lectins initiated the appointment of Mike Capaldi to explore their localization further in diseased muscle [24][25][26][27] . These studies identified a missing band with gel electrophoresis, in one case, using the lectin Ricinus Communis I which was later shown by Jonathan Wadsworth to be laminin α2.…”

Section: Laboratory Muscle Research At Hammersmith Hospital By the Dubowitz Groupmentioning

confidence: 99%

Historical aspects of muscle research in the Dubowitz Neuromuscular Centre: the Hammersmith days

Sewry

2021

Neuromuscular Disorders

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Binding of Ricinus communis I lectin to the muscle cell plasma membrane in diseased muscle

Cited by 15 publications

References 10 publications

Lectin Staining of Extensor Digitorum Longus Muscle Cell Membranes in Alloxan Diabetic Rats

Lectin Staining of Extensor Digitorum Longus Muscle Cell Membranes in Alloxan Diabetic Rats

Lysosomal glycogen storage disease without acid maltase deficiency. A lectin-histochemical study of an unusual type of lysosomal glycogen storage disease.

Historical aspects of muscle research in the Dubowitz Neuromuscular Centre: the Hammersmith days

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